Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets
There is some evidence that the two rodent insulin genes are differentially regulated in mice, although there is no satisfactory consensus on the relative levels and patterns of expression for the two genes. Using the mouse insulinoma cell line MIN6, we have demonstrated by quantitative RT-PCR, diff...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2002-08, Vol.296 (3), p.589-595 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 595 |
|---|---|
| container_issue | 3 |
| container_start_page | 589 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 296 |
| creator | Roderigo-Milne, Helen Hauge-Evans, Astrid C Persaud, Shanta J Jones, Peter M |
| description | There is some evidence that the two rodent insulin genes are differentially regulated in mice, although there is no satisfactory consensus on the relative levels and patterns of expression for the two genes. Using the mouse insulinoma cell line MIN6, we have demonstrated by quantitative RT-PCR, differential patterns of expression for the two genes. In mouse islets and early passage MIN6 cells, expression of ins 1 and ins 2 were found to be approximately equal, but levels of ins 1 mRNA diminished rapidly with continued passage. Furthermore, the ins 1 gene was found to be up-regulated in response to glucose stimulation and as a result of increased cell–cell contact, but no effect on the ins 2 gene was observed. Since the MIN6 cell line is frequently used as a β-cell model for gene expression studies, consideration should be given to both insulin genes. |
| doi_str_mv | 10.1016/S0006-291X(02)00913-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71988802</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X02009130</els_id><sourcerecordid>21186935</sourcerecordid><originalsourceid>FETCH-LOGICAL-c458t-4c3e16871955980690c676ddbf5e19b3ed82236f35d1c266916510774741e7f23</originalsourceid><addsrcrecordid>eNqFkMtKxEAQRRtRdHx8gtIr0UW0qpN00iuR8Qk-Fiq4azKdirRkkrErEf17Mw906argcqpucYTYRzhBQH36BAA6UgZfj0AdAxiMI1gTIwQDkUJI1sXoF9kS28zvAIiJNptiCxVmGpQaiccLX1UUqOl8UUv6mgVi9m0j20r6hvvaN_KNGmKJsmhKqYZU3t8-aOmornmRzZj6svVcU8e7YqMqaqa91dwRL1eXz-Ob6O7x-nZ8fhe5JM27KHExoc4zNGlqctAGnM50WU6qlNBMYipzpWJdxWmJTmltUKcIWZZkCVJWqXhHHC7vzkL70RN3dup5_lLRUNuzHS7neQ7_gwox1yZOBzBdgi60zIEqOwt-WoRvi2Dnyu1CuZ37tKDsQrmFYe9gVdBPplT-ba0cD8DZEqDBx6enYNl5ahyVPpDr7KDun4of0ySN-w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21186935</pqid></control><display><type>article</type><title>Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Roderigo-Milne, Helen ; Hauge-Evans, Astrid C ; Persaud, Shanta J ; Jones, Peter M</creator><creatorcontrib>Roderigo-Milne, Helen ; Hauge-Evans, Astrid C ; Persaud, Shanta J ; Jones, Peter M</creatorcontrib><description>There is some evidence that the two rodent insulin genes are differentially regulated in mice, although there is no satisfactory consensus on the relative levels and patterns of expression for the two genes. Using the mouse insulinoma cell line MIN6, we have demonstrated by quantitative RT-PCR, differential patterns of expression for the two genes. In mouse islets and early passage MIN6 cells, expression of ins 1 and ins 2 were found to be approximately equal, but levels of ins 1 mRNA diminished rapidly with continued passage. Furthermore, the ins 1 gene was found to be up-regulated in response to glucose stimulation and as a result of increased cell–cell contact, but no effect on the ins 2 gene was observed. Since the MIN6 cell line is frequently used as a β-cell model for gene expression studies, consideration should be given to both insulin genes.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(02)00913-0</identifier><identifier>PMID: 12176022</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Communication ; Cell Culture Techniques ; Cell Line, Transformed ; Cells, Cultured ; Gene expression ; Gene Expression Regulation ; Glucose - pharmacology ; ins 1 ; ins 2 ; Insulin - biosynthesis ; Insulin - genetics ; Insulinoma ; Islets of Langerhans - drug effects ; Islets of Langerhans - metabolism ; Mice ; MIN6 ; Mouse ; mRNA ; PCR ; Pseudoislet ; RNA, Messenger - biosynthesis ; Transcription, Genetic</subject><ispartof>Biochemical and biophysical research communications, 2002-08, Vol.296 (3), p.589-595</ispartof><rights>2002 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-4c3e16871955980690c676ddbf5e19b3ed82236f35d1c266916510774741e7f23</citedby><cites>FETCH-LOGICAL-c458t-4c3e16871955980690c676ddbf5e19b3ed82236f35d1c266916510774741e7f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(02)00913-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12176022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roderigo-Milne, Helen</creatorcontrib><creatorcontrib>Hauge-Evans, Astrid C</creatorcontrib><creatorcontrib>Persaud, Shanta J</creatorcontrib><creatorcontrib>Jones, Peter M</creatorcontrib><title>Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>There is some evidence that the two rodent insulin genes are differentially regulated in mice, although there is no satisfactory consensus on the relative levels and patterns of expression for the two genes. Using the mouse insulinoma cell line MIN6, we have demonstrated by quantitative RT-PCR, differential patterns of expression for the two genes. In mouse islets and early passage MIN6 cells, expression of ins 1 and ins 2 were found to be approximately equal, but levels of ins 1 mRNA diminished rapidly with continued passage. Furthermore, the ins 1 gene was found to be up-regulated in response to glucose stimulation and as a result of increased cell–cell contact, but no effect on the ins 2 gene was observed. Since the MIN6 cell line is frequently used as a β-cell model for gene expression studies, consideration should be given to both insulin genes.</description><subject>Animals</subject><subject>Cell Communication</subject><subject>Cell Culture Techniques</subject><subject>Cell Line, Transformed</subject><subject>Cells, Cultured</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Glucose - pharmacology</subject><subject>ins 1</subject><subject>ins 2</subject><subject>Insulin - biosynthesis</subject><subject>Insulin - genetics</subject><subject>Insulinoma</subject><subject>Islets of Langerhans - drug effects</subject><subject>Islets of Langerhans - metabolism</subject><subject>Mice</subject><subject>MIN6</subject><subject>Mouse</subject><subject>mRNA</subject><subject>PCR</subject><subject>Pseudoislet</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Transcription, Genetic</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxEAQRRtRdHx8gtIr0UW0qpN00iuR8Qk-Fiq4azKdirRkkrErEf17Mw906argcqpucYTYRzhBQH36BAA6UgZfj0AdAxiMI1gTIwQDkUJI1sXoF9kS28zvAIiJNptiCxVmGpQaiccLX1UUqOl8UUv6mgVi9m0j20r6hvvaN_KNGmKJsmhKqYZU3t8-aOmornmRzZj6svVcU8e7YqMqaqa91dwRL1eXz-Ob6O7x-nZ8fhe5JM27KHExoc4zNGlqctAGnM50WU6qlNBMYipzpWJdxWmJTmltUKcIWZZkCVJWqXhHHC7vzkL70RN3dup5_lLRUNuzHS7neQ7_gwox1yZOBzBdgi60zIEqOwt-WoRvi2Dnyu1CuZ37tKDsQrmFYe9gVdBPplT-ba0cD8DZEqDBx6enYNl5ahyVPpDr7KDun4of0ySN-w</recordid><startdate>20020823</startdate><enddate>20020823</enddate><creator>Roderigo-Milne, Helen</creator><creator>Hauge-Evans, Astrid C</creator><creator>Persaud, Shanta J</creator><creator>Jones, Peter M</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020823</creationdate><title>Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets</title><author>Roderigo-Milne, Helen ; Hauge-Evans, Astrid C ; Persaud, Shanta J ; Jones, Peter M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-4c3e16871955980690c676ddbf5e19b3ed82236f35d1c266916510774741e7f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Cell Communication</topic><topic>Cell Culture Techniques</topic><topic>Cell Line, Transformed</topic><topic>Cells, Cultured</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Glucose - pharmacology</topic><topic>ins 1</topic><topic>ins 2</topic><topic>Insulin - biosynthesis</topic><topic>Insulin - genetics</topic><topic>Insulinoma</topic><topic>Islets of Langerhans - drug effects</topic><topic>Islets of Langerhans - metabolism</topic><topic>Mice</topic><topic>MIN6</topic><topic>Mouse</topic><topic>mRNA</topic><topic>PCR</topic><topic>Pseudoislet</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roderigo-Milne, Helen</creatorcontrib><creatorcontrib>Hauge-Evans, Astrid C</creatorcontrib><creatorcontrib>Persaud, Shanta J</creatorcontrib><creatorcontrib>Jones, Peter M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roderigo-Milne, Helen</au><au>Hauge-Evans, Astrid C</au><au>Persaud, Shanta J</au><au>Jones, Peter M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-08-23</date><risdate>2002</risdate><volume>296</volume><issue>3</issue><spage>589</spage><epage>595</epage><pages>589-595</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>There is some evidence that the two rodent insulin genes are differentially regulated in mice, although there is no satisfactory consensus on the relative levels and patterns of expression for the two genes. Using the mouse insulinoma cell line MIN6, we have demonstrated by quantitative RT-PCR, differential patterns of expression for the two genes. In mouse islets and early passage MIN6 cells, expression of ins 1 and ins 2 were found to be approximately equal, but levels of ins 1 mRNA diminished rapidly with continued passage. Furthermore, the ins 1 gene was found to be up-regulated in response to glucose stimulation and as a result of increased cell–cell contact, but no effect on the ins 2 gene was observed. Since the MIN6 cell line is frequently used as a β-cell model for gene expression studies, consideration should be given to both insulin genes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12176022</pmid><doi>10.1016/S0006-291X(02)00913-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2002-08, Vol.296 (3), p.589-595 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71988802 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Communication Cell Culture Techniques Cell Line, Transformed Cells, Cultured Gene expression Gene Expression Regulation Glucose - pharmacology ins 1 ins 2 Insulin - biosynthesis Insulin - genetics Insulinoma Islets of Langerhans - drug effects Islets of Langerhans - metabolism Mice MIN6 Mouse mRNA PCR Pseudoislet RNA, Messenger - biosynthesis Transcription, Genetic |
| title | Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T18%3A38%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20expression%20of%20insulin%20genes%201%20and%202%20in%20MIN6%20cells%20and%20pseudoislets&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Roderigo-Milne,%20Helen&rft.date=2002-08-23&rft.volume=296&rft.issue=3&rft.spage=589&rft.epage=595&rft.pages=589-595&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/S0006-291X(02)00913-0&rft_dat=%3Cproquest_cross%3E21186935%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21186935&rft_id=info:pmid/12176022&rft_els_id=S0006291X02009130&rfr_iscdi=true |