Determination of surface-bound-fluorophore orientation by goniometric fluorescence polarization: application to quantification of DNA-chip readouts

We present a polarized goniofluorimeter designed to measure the observation-angle and polarization-dependent intensity emitted by a group of surface-bound fluorescent molecules. We studied two types of surface bonding: In one case, dyes were adsorbed into the surface by spin coating, and in the othe...

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Veröffentlicht in:	Applied Optics 2002-08, Vol.41 (22), p.4732-4738
Hauptverfasser:	Barritault, Pierre, Gétin, Stéphane, Chaton, Patrick, Vinet, Françoise, Fouqué, Brigitte
Format:	Artikel
Sprache:	eng
Schlagworte:	Computer Simulation DNA Dyes Fluorescence Fluorescence Polarization Fluorescent Dyes Goniometers Humans Light polarization Models, Theoretical Oligonucleotide Array Sequence Analysis Readout systems Silicon wafers
Online-Zugang:	Volltext
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