Giardia lamblia disrupts tight junctional ZO-1 and increases permeability in non-transformed human small intestinal epithelial monolayers: effects of epidermal growth factor
In order to improve our understanding of the host cell–parasite interactions in giardiasis, this study assessed the effects of Giardia lamblia on epithelial permeability and tight junctional ZO-1, determined whether epidermal growth factor (EGF) may affect Giardia-induced epithelial injury, and eval...
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| Veröffentlicht in: | Parasitology 2002-07, Vol.125 (1), p.11-19 |
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| creator | BURET, A. G. MITCHELL, K. MUENCH, D. G. SCOTT, K. G. E. |
| description | In order to improve our understanding of the host cell–parasite interactions in giardiasis, this study assessed the effects of Giardia lamblia on epithelial permeability and tight junctional ZO-1, determined whether epidermal growth factor (EGF) may affect Giardia-induced epithelial injury, and evaluated if EGF modulates epithelial colonization by live G. lamblia trophozoites. Permeability was assessed in assays of trans-epithelial fluxes of FITC-dextran, and ZO-1 integrity was characterized by confocal laser immunofluorescence microscopy in confluent epithelial cell monolayers. G. lamblia significantly increased paracellular permeability and disrupted tight-junctional ZO-1 of a novel non-transformed human small intestinal epithelial cell line (SCBN). Pre-treatment with EGF prevented the development of these abnormalities and significantly inhibited attachment of live trophozoites to the enterocytes, independently of a direct microbiocidal action. These findings demonstrate that G. lamblia may cause intestinal pathophysiology by disrupting tight junctional ZO-1 and increasing epithelial permeability. Apical administration of EGF prevents these abnormalities, and reduces epithelial colonization by the live parasites. |
| doi_str_mv | 10.1017/S0031182002001853 |
| format | Article |
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G. ; MITCHELL, K. ; MUENCH, D. G. ; SCOTT, K. G. E.</creator><creatorcontrib>BURET, A. G. ; MITCHELL, K. ; MUENCH, D. G. ; SCOTT, K. G. E.</creatorcontrib><description>In order to improve our understanding of the host cell–parasite interactions in giardiasis, this study assessed the effects of Giardia lamblia on epithelial permeability and tight junctional ZO-1, determined whether epidermal growth factor (EGF) may affect Giardia-induced epithelial injury, and evaluated if EGF modulates epithelial colonization by live G. lamblia trophozoites. Permeability was assessed in assays of trans-epithelial fluxes of FITC-dextran, and ZO-1 integrity was characterized by confocal laser immunofluorescence microscopy in confluent epithelial cell monolayers. G. lamblia significantly increased paracellular permeability and disrupted tight-junctional ZO-1 of a novel non-transformed human small intestinal epithelial cell line (SCBN). Pre-treatment with EGF prevented the development of these abnormalities and significantly inhibited attachment of live trophozoites to the enterocytes, independently of a direct microbiocidal action. These findings demonstrate that G. lamblia may cause intestinal pathophysiology by disrupting tight junctional ZO-1 and increasing epithelial permeability. Apical administration of EGF prevents these abnormalities, and reduces epithelial colonization by the live parasites.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182002001853</identifier><identifier>PMID: 12166516</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Biological and medical sciences ; Cell Membrane Permeability - physiology ; Colonization ; epidermal growth factor ; Epidermal Growth Factor - pharmacology ; Epithelial Cells ; epithelial permeability ; Experimental protozoal diseases and models ; Giardia ; Giardia lamblia - growth & development ; Giardia lamblia - metabolism ; Giardiasis - metabolism ; Giardiasis - parasitology ; Giardiasis - pathology ; Host-Parasite Interactions ; Humans ; Infectious diseases ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - parasitology ; Intestinal Mucosa - pathology ; intestinal pathophysiology ; Intestine, Small - metabolism ; Intestine, Small - parasitology ; Intestine, Small - pathology ; Medical sciences ; Membrane Proteins - metabolism ; Microscopy, Confocal ; Parasites ; Parasitic diseases ; Permeability ; Phosphoproteins - metabolism ; Protozoal diseases ; Tight Junctions - physiology ; ZO-1 ; Zonula Occludens-1 Protein</subject><ispartof>Parasitology, 2002-07, Vol.125 (1), p.11-19</ispartof><rights>2002 Cambridge University Press</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-b32a8125926188ad24c539d10d65c4e6f533cc8495803182907d3b3aad0739873</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182002001853/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13763691$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12166516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BURET, A. G.</creatorcontrib><creatorcontrib>MITCHELL, K.</creatorcontrib><creatorcontrib>MUENCH, D. G.</creatorcontrib><creatorcontrib>SCOTT, K. G. E.</creatorcontrib><title>Giardia lamblia disrupts tight junctional ZO-1 and increases permeability in non-transformed human small intestinal epithelial monolayers: effects of epidermal growth factor</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>In order to improve our understanding of the host cell–parasite interactions in giardiasis, this study assessed the effects of Giardia lamblia on epithelial permeability and tight junctional ZO-1, determined whether epidermal growth factor (EGF) may affect Giardia-induced epithelial injury, and evaluated if EGF modulates epithelial colonization by live G. lamblia trophozoites. Permeability was assessed in assays of trans-epithelial fluxes of FITC-dextran, and ZO-1 integrity was characterized by confocal laser immunofluorescence microscopy in confluent epithelial cell monolayers. G. lamblia significantly increased paracellular permeability and disrupted tight-junctional ZO-1 of a novel non-transformed human small intestinal epithelial cell line (SCBN). Pre-treatment with EGF prevented the development of these abnormalities and significantly inhibited attachment of live trophozoites to the enterocytes, independently of a direct microbiocidal action. These findings demonstrate that G. lamblia may cause intestinal pathophysiology by disrupting tight junctional ZO-1 and increasing epithelial permeability. Apical administration of EGF prevents these abnormalities, and reduces epithelial colonization by the live parasites.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane Permeability - physiology</subject><subject>Colonization</subject><subject>epidermal growth factor</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Epithelial Cells</subject><subject>epithelial permeability</subject><subject>Experimental protozoal diseases and models</subject><subject>Giardia</subject><subject>Giardia lamblia - growth & development</subject><subject>Giardia lamblia - metabolism</subject><subject>Giardiasis - metabolism</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - pathology</subject><subject>Host-Parasite Interactions</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - parasitology</subject><subject>Intestinal Mucosa - pathology</subject><subject>intestinal pathophysiology</subject><subject>Intestine, Small - metabolism</subject><subject>Intestine, Small - parasitology</subject><subject>Intestine, Small - pathology</subject><subject>Medical sciences</subject><subject>Membrane Proteins - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Permeability</subject><subject>Phosphoproteins - metabolism</subject><subject>Protozoal diseases</subject><subject>Tight Junctions - physiology</subject><subject>ZO-1</subject><subject>Zonula Occludens-1 Protein</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFkt-K1DAUxoso7rj6AN5IEPSumpM0aerdsqujsrCKiuBNyaTpTMa0qUmKzkP5jp5hBgcUEQIH8v3On5wvRfEQ6DOgUD__QCkHUIxSPKAEv1UsoJJNqUDC7WKxl8u9flbcS2lLKZVcsrvFGTCQUoBcFD-XTsfOaeL1sPIYO5fiPOVEsltvMtnOo8kujNqTLzclED12xI0mWp1sIpONg9Ur513e4TUZw1jmqMfUBxQ6spkHPZI0aO9RzjZlt69kJ5c3Frt5MoQxeL2zMb0gtu-twc6h3xMd1kZgHcP3vCG9NjnE-8WdXvtkHxzjefHp1cuPl6_L65vlm8uL69IIELlccaYVMNEwCUrpjlVG8KYD2klhKit7wbkxqmqEwg0p1tC64yuudUdr3qianxdPD3WnGL7NOHY7uGSs93q0YU5tDY1iouL_BUFJpoALBB__AW7DHHEZqWVoGeOcVwjBATIxpBRt307RDTruWqDt3vH2L8cx59Gx8LzClZ8yjhYj8OQI6GS079Ee49KJ4zV-igaQKw-cS9n--K3r-LWVNa9FK5fv22X1-epKNO_at8jz47D4c6Lr1vb0pH-P-wvM_NLl</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>BURET, A. G.</creator><creator>MITCHELL, K.</creator><creator>MUENCH, D. G.</creator><creator>SCOTT, K. G. 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G.</au><au>MITCHELL, K.</au><au>MUENCH, D. G.</au><au>SCOTT, K. G. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Giardia lamblia disrupts tight junctional ZO-1 and increases permeability in non-transformed human small intestinal epithelial monolayers: effects of epidermal growth factor</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>125</volume><issue>1</issue><spage>11</spage><epage>19</epage><pages>11-19</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>In order to improve our understanding of the host cell–parasite interactions in giardiasis, this study assessed the effects of Giardia lamblia on epithelial permeability and tight junctional ZO-1, determined whether epidermal growth factor (EGF) may affect Giardia-induced epithelial injury, and evaluated if EGF modulates epithelial colonization by live G. lamblia trophozoites. Permeability was assessed in assays of trans-epithelial fluxes of FITC-dextran, and ZO-1 integrity was characterized by confocal laser immunofluorescence microscopy in confluent epithelial cell monolayers. G. lamblia significantly increased paracellular permeability and disrupted tight-junctional ZO-1 of a novel non-transformed human small intestinal epithelial cell line (SCBN). Pre-treatment with EGF prevented the development of these abnormalities and significantly inhibited attachment of live trophozoites to the enterocytes, independently of a direct microbiocidal action. These findings demonstrate that G. lamblia may cause intestinal pathophysiology by disrupting tight junctional ZO-1 and increasing epithelial permeability. Apical administration of EGF prevents these abnormalities, and reduces epithelial colonization by the live parasites.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>12166516</pmid><doi>10.1017/S0031182002001853</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cell Membrane Permeability - physiology Colonization epidermal growth factor Epidermal Growth Factor - pharmacology Epithelial Cells epithelial permeability Experimental protozoal diseases and models Giardia Giardia lamblia - growth & development Giardia lamblia - metabolism Giardiasis - metabolism Giardiasis - parasitology Giardiasis - pathology Host-Parasite Interactions Humans Infectious diseases Intestinal Mucosa - metabolism Intestinal Mucosa - parasitology Intestinal Mucosa - pathology intestinal pathophysiology Intestine, Small - metabolism Intestine, Small - parasitology Intestine, Small - pathology Medical sciences Membrane Proteins - metabolism Microscopy, Confocal Parasites Parasitic diseases Permeability Phosphoproteins - metabolism Protozoal diseases Tight Junctions - physiology ZO-1 Zonula Occludens-1 Protein |
| title | Giardia lamblia disrupts tight junctional ZO-1 and increases permeability in non-transformed human small intestinal epithelial monolayers: effects of epidermal growth factor |
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