Comparison between High-Performance Liquid Chromatography and Enzyme-linked Immunosorbent Assay for the Determination of 8-Hydroxy-2′-deoxyguanosine in Human Urine
8-Hydroxy-2′-deoxyguanosine (8-OH-dG), which has been regarded as a potential marker of oxidative DNA damage induced by reactive oxygen species, was measured in human urine by a commercial ELISA using a monoclonal antibody N45.1 and by high-performance liquid chromatography (HPLC) coupled to an elec...
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| Veröffentlicht in: | Cancer epidemiology, biomarkers & prevention biomarkers & prevention, 2002-08, Vol.11 (8), p.767-770 |
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| creator | SHIMOI, Kayoko KASAI, Hiroshi YOKOTA, Naoko TOYOKUNI, Shinya KINAE, Naohide |
| description | 8-Hydroxy-2′-deoxyguanosine (8-OH-dG), which has been regarded as a potential marker of oxidative DNA damage induced by reactive
oxygen species, was measured in human urine by a commercial ELISA using a monoclonal antibody N45.1 and by high-performance
liquid chromatography (HPLC) coupled to an electrochemical detector (HPLC-ECD) to evaluate whether the ELISA system is applicable
to human monitoring studies. The urine samples were collected from 120 healthy men ages 18–58 in a steel-manufacturing company.
A good correlation ( r = 0.833; P < 0.0001) was observed between the two methods on HPLC-purified 8-OH-dG fractions from 23 urine samples. The mean value (±SD)
of 8-OH-dG (μg/g creatinine) was 5.47 (±2.97) by HPLC-ECD assay and 5.50 (±2.36) by ELISA. However, the correlation ( r ) between the two methods on 120 original urine samples was 0.460 [ P < 0.001; mean value (±SD) of 8-OH-dG (μg/g creatinine) was 4.46 (±2.03) by the HPLC assay and 9.33 (±3.23) by ELISA]. ELISA
estimates were about 2-fold higher than the HPLC estimates on original urine. For an unknown reason, 10% of the urine samples
showed more than a 4-fold increase in value by ELISA. It is suggested that the ELISA system is applicable for comparative
human monitoring studies. Prepurification of ‘samples is required to determine the absolute value of 8-OH-dG in individual
urine samples by ELISA. |
| format | Article |
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oxygen species, was measured in human urine by a commercial ELISA using a monoclonal antibody N45.1 and by high-performance
liquid chromatography (HPLC) coupled to an electrochemical detector (HPLC-ECD) to evaluate whether the ELISA system is applicable
to human monitoring studies. The urine samples were collected from 120 healthy men ages 18–58 in a steel-manufacturing company.
A good correlation ( r = 0.833; P < 0.0001) was observed between the two methods on HPLC-purified 8-OH-dG fractions from 23 urine samples. The mean value (±SD)
of 8-OH-dG (μg/g creatinine) was 5.47 (±2.97) by HPLC-ECD assay and 5.50 (±2.36) by ELISA. However, the correlation ( r ) between the two methods on 120 original urine samples was 0.460 [ P < 0.001; mean value (±SD) of 8-OH-dG (μg/g creatinine) was 4.46 (±2.03) by the HPLC assay and 9.33 (±3.23) by ELISA]. ELISA
estimates were about 2-fold higher than the HPLC estimates on original urine. For an unknown reason, 10% of the urine samples
showed more than a 4-fold increase in value by ELISA. It is suggested that the ELISA system is applicable for comparative
human monitoring studies. Prepurification of ‘samples is required to determine the absolute value of 8-OH-dG in individual
urine samples by ELISA.</description><identifier>ISSN: 1055-9965</identifier><identifier>EISSN: 1538-7755</identifier><identifier>PMID: 12163331</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Biomarkers - analysis ; Chromatography, High Pressure Liquid ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - urine ; DNA Damage ; Enzyme-Linked Immunosorbent Assay ; Host-tumor relations. Immunology. Biological markers ; Humans ; Male ; Medical sciences ; Middle Aged ; Sensitivity and Specificity ; Specimen Handling ; Tumors ; Urinalysis</subject><ispartof>Cancer epidemiology, biomarkers & prevention, 2002-08, Vol.11 (8), p.767-770</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13842197$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12163331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHIMOI, Kayoko</creatorcontrib><creatorcontrib>KASAI, Hiroshi</creatorcontrib><creatorcontrib>YOKOTA, Naoko</creatorcontrib><creatorcontrib>TOYOKUNI, Shinya</creatorcontrib><creatorcontrib>KINAE, Naohide</creatorcontrib><title>Comparison between High-Performance Liquid Chromatography and Enzyme-linked Immunosorbent Assay for the Determination of 8-Hydroxy-2′-deoxyguanosine in Human Urine</title><title>Cancer epidemiology, biomarkers & prevention</title><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><description>8-Hydroxy-2′-deoxyguanosine (8-OH-dG), which has been regarded as a potential marker of oxidative DNA damage induced by reactive
oxygen species, was measured in human urine by a commercial ELISA using a monoclonal antibody N45.1 and by high-performance
liquid chromatography (HPLC) coupled to an electrochemical detector (HPLC-ECD) to evaluate whether the ELISA system is applicable
to human monitoring studies. The urine samples were collected from 120 healthy men ages 18–58 in a steel-manufacturing company.
A good correlation ( r = 0.833; P < 0.0001) was observed between the two methods on HPLC-purified 8-OH-dG fractions from 23 urine samples. The mean value (±SD)
of 8-OH-dG (μg/g creatinine) was 5.47 (±2.97) by HPLC-ECD assay and 5.50 (±2.36) by ELISA. However, the correlation ( r ) between the two methods on 120 original urine samples was 0.460 [ P < 0.001; mean value (±SD) of 8-OH-dG (μg/g creatinine) was 4.46 (±2.03) by the HPLC assay and 9.33 (±3.23) by ELISA]. ELISA
estimates were about 2-fold higher than the HPLC estimates on original urine. For an unknown reason, 10% of the urine samples
showed more than a 4-fold increase in value by ELISA. It is suggested that the ELISA system is applicable for comparative
human monitoring studies. Prepurification of ‘samples is required to determine the absolute value of 8-OH-dG in individual
urine samples by ELISA.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - urine</subject><subject>DNA Damage</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Host-tumor relations. Immunology. Biological markers</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Sensitivity and Specificity</subject><subject>Specimen Handling</subject><subject>Tumors</subject><subject>Urinalysis</subject><issn>1055-9965</issn><issn>1538-7755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9O3DAQhyNUBHTbV0C-tD1ZiuM4do5o-bNIK7UHOEeTeLIxje3FTgThxMv0BfpIfZK6YlFPMyN985vRd5SdMcEVlVKID6nPhaB1XYnT7GOMD3mey1qIk-yUFazinLOz7Nfa2z0EE70jLU5PiI5szG6gPzD0PlhwHZKteZyNJusheAuT3wXYDwsBp8mVe1ks0tG4n6jJrbWz89GHFt1ELmKEhaQQMg1ILnHCYI2DyaRTvieKbhYd_PNCiz-vv6nG1O5mSPvGITHpjTldJ_chjZ-y4x7GiJ8PdZXdX1_drTd0-_3mdn2xpUNR1ROFQkBZqlLpiouyhlL3WqsWOVcSZIusB6163mNedW3HpdZ5rqTOW8bKgpeCr7Kvb7n74B9njFNjTexwHMGhn2MjWS1rlcytsvMDOLcWdbMPxkJYmnexCfhyACB2MPYhiTTxP8dVWaSwxH1744Yk_ckEbLp_ykPAiBC6oWGsUY2sJP8LLtSVIA</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>SHIMOI, Kayoko</creator><creator>KASAI, Hiroshi</creator><creator>YOKOTA, Naoko</creator><creator>TOYOKUNI, Shinya</creator><creator>KINAE, Naohide</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>Comparison between High-Performance Liquid Chromatography and Enzyme-linked Immunosorbent Assay for the Determination of 8-Hydroxy-2′-deoxyguanosine in Human Urine</title><author>SHIMOI, Kayoko ; KASAI, Hiroshi ; YOKOTA, Naoko ; TOYOKUNI, Shinya ; KINAE, Naohide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-a25a44848d63549a4dfdd8be3387a7be1fad8f3fe06cbc37dd0087d0b11423453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - urine</topic><topic>DNA Damage</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Host-tumor relations. Immunology. Biological markers</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Sensitivity and Specificity</topic><topic>Specimen Handling</topic><topic>Tumors</topic><topic>Urinalysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHIMOI, Kayoko</creatorcontrib><creatorcontrib>KASAI, Hiroshi</creatorcontrib><creatorcontrib>YOKOTA, Naoko</creatorcontrib><creatorcontrib>TOYOKUNI, Shinya</creatorcontrib><creatorcontrib>KINAE, Naohide</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHIMOI, Kayoko</au><au>KASAI, Hiroshi</au><au>YOKOTA, Naoko</au><au>TOYOKUNI, Shinya</au><au>KINAE, Naohide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison between High-Performance Liquid Chromatography and Enzyme-linked Immunosorbent Assay for the Determination of 8-Hydroxy-2′-deoxyguanosine in Human Urine</atitle><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>11</volume><issue>8</issue><spage>767</spage><epage>770</epage><pages>767-770</pages><issn>1055-9965</issn><eissn>1538-7755</eissn><abstract>8-Hydroxy-2′-deoxyguanosine (8-OH-dG), which has been regarded as a potential marker of oxidative DNA damage induced by reactive
oxygen species, was measured in human urine by a commercial ELISA using a monoclonal antibody N45.1 and by high-performance
liquid chromatography (HPLC) coupled to an electrochemical detector (HPLC-ECD) to evaluate whether the ELISA system is applicable
to human monitoring studies. The urine samples were collected from 120 healthy men ages 18–58 in a steel-manufacturing company.
A good correlation ( r = 0.833; P < 0.0001) was observed between the two methods on HPLC-purified 8-OH-dG fractions from 23 urine samples. The mean value (±SD)
of 8-OH-dG (μg/g creatinine) was 5.47 (±2.97) by HPLC-ECD assay and 5.50 (±2.36) by ELISA. However, the correlation ( r ) between the two methods on 120 original urine samples was 0.460 [ P < 0.001; mean value (±SD) of 8-OH-dG (μg/g creatinine) was 4.46 (±2.03) by the HPLC assay and 9.33 (±3.23) by ELISA]. ELISA
estimates were about 2-fold higher than the HPLC estimates on original urine. For an unknown reason, 10% of the urine samples
showed more than a 4-fold increase in value by ELISA. It is suggested that the ELISA system is applicable for comparative
human monitoring studies. Prepurification of ‘samples is required to determine the absolute value of 8-OH-dG in individual
urine samples by ELISA.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>12163331</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Cancer epidemiology, biomarkers & prevention, 2002-08, Vol.11 (8), p.767-770 |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Adolescent Adult Biological and medical sciences Biomarkers - analysis Chromatography, High Pressure Liquid Deoxyguanosine - analogs & derivatives Deoxyguanosine - urine DNA Damage Enzyme-Linked Immunosorbent Assay Host-tumor relations. Immunology. Biological markers Humans Male Medical sciences Middle Aged Sensitivity and Specificity Specimen Handling Tumors Urinalysis |
| title | Comparison between High-Performance Liquid Chromatography and Enzyme-linked Immunosorbent Assay for the Determination of 8-Hydroxy-2′-deoxyguanosine in Human Urine |
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