Tissue tropism of the TTV in experimentally infected rhesus monkeys
To determine whether the transfusion-transmitted virus (TTV) is hepatotropic. Total DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes. The double-...
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| Veröffentlicht in: | Chinese medical journal 2002-07, Vol.115 (7), p.1088-1090 |
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| creator | Xiao, Hong Luo, Kangxian Yang, Shouchang Wang, Yanjun Liang, Weifang |
| description | To determine whether the transfusion-transmitted virus (TTV) is hepatotropic.
Total DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes.
The double-stranded probe was hybridized with DNA from the liver, bone marrow, spleen,stomach, small intestine and colon. The single-stranded antisense probe was hybridized with DNA from the liver, small intestine and bone marrow of all 5 monkeys, but not with that from other tissues.
As the viral genome was of negative polarity, the plus-stranded fragment identified in our study might be a replicative intermediate, and was only demonstrated in the liver, small intestine, and bone marrow by dot blot hybridization with single-stranded antisense probes. It is suggested that the TTV replicates in the liver, bone marrow and small intestine, and TTV might be hepatotropic. |
| format | Article |
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Total DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes.
The double-stranded probe was hybridized with DNA from the liver, bone marrow, spleen,stomach, small intestine and colon. The single-stranded antisense probe was hybridized with DNA from the liver, small intestine and bone marrow of all 5 monkeys, but not with that from other tissues.
As the viral genome was of negative polarity, the plus-stranded fragment identified in our study might be a replicative intermediate, and was only demonstrated in the liver, small intestine, and bone marrow by dot blot hybridization with single-stranded antisense probes. It is suggested that the TTV replicates in the liver, bone marrow and small intestine, and TTV might be hepatotropic.</description><identifier>ISSN: 0366-6999</identifier><identifier>PMID: 12173599</identifier><language>eng</language><publisher>China</publisher><subject>Animals ; Bone Marrow - virology ; DNA Virus Infections - virology ; DNA, Viral - analysis ; Intestine, Small - virology ; Liver - virology ; Macaca mulatta ; Torque teno virus - genetics ; Torque teno virus - isolation & purification ; Torque teno virus - physiology ; Virus Replication</subject><ispartof>Chinese medical journal, 2002-07, Vol.115 (7), p.1088-1090</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12173599$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiao, Hong</creatorcontrib><creatorcontrib>Luo, Kangxian</creatorcontrib><creatorcontrib>Yang, Shouchang</creatorcontrib><creatorcontrib>Wang, Yanjun</creatorcontrib><creatorcontrib>Liang, Weifang</creatorcontrib><title>Tissue tropism of the TTV in experimentally infected rhesus monkeys</title><title>Chinese medical journal</title><addtitle>Chin Med J (Engl)</addtitle><description>To determine whether the transfusion-transmitted virus (TTV) is hepatotropic.
Total DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes.
The double-stranded probe was hybridized with DNA from the liver, bone marrow, spleen,stomach, small intestine and colon. The single-stranded antisense probe was hybridized with DNA from the liver, small intestine and bone marrow of all 5 monkeys, but not with that from other tissues.
As the viral genome was of negative polarity, the plus-stranded fragment identified in our study might be a replicative intermediate, and was only demonstrated in the liver, small intestine, and bone marrow by dot blot hybridization with single-stranded antisense probes. It is suggested that the TTV replicates in the liver, bone marrow and small intestine, and TTV might be hepatotropic.</description><subject>Animals</subject><subject>Bone Marrow - virology</subject><subject>DNA Virus Infections - virology</subject><subject>DNA, Viral - analysis</subject><subject>Intestine, Small - virology</subject><subject>Liver - virology</subject><subject>Macaca mulatta</subject><subject>Torque teno virus - genetics</subject><subject>Torque teno virus - isolation & purification</subject><subject>Torque teno virus - physiology</subject><subject>Virus Replication</subject><issn>0366-6999</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1j0tLxDAUhbNQZsYZ_4Jk5a6Q3DRJs5TiCwbcVLcl094w1b7sTcH-ewuOqwOHj8P5rthOKGMS45zbshuiTyFAa2s2bCtBWqWd27G8aIhm5HEaxoY6PgQez8iL4oM3PcefEaemwz76tl3WJmAVsebTGWkm3g39Fy50YNfBt4S3l9yz96fHIn9Jjm_Pr_nDMRlB2Jhk2skMMlFLnVZBBaHAB0BAI6X30nmFqUyFNw5MmmkRUAkL62lRmRP4VO3Z_d_uOA3fM1Isu4YqbFvf4zBTaaWzmQK1gncXcD51WJfj6uCnpfzXVr92I1HC</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Xiao, Hong</creator><creator>Luo, Kangxian</creator><creator>Yang, Shouchang</creator><creator>Wang, Yanjun</creator><creator>Liang, Weifang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Tissue tropism of the TTV in experimentally infected rhesus monkeys</title><author>Xiao, Hong ; Luo, Kangxian ; Yang, Shouchang ; Wang, Yanjun ; Liang, Weifang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-85918280d154cf3f032af2e2e611aa19a3e4140a69264850fe30720020c6b2a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Bone Marrow - virology</topic><topic>DNA Virus Infections - virology</topic><topic>DNA, Viral - analysis</topic><topic>Intestine, Small - virology</topic><topic>Liver - virology</topic><topic>Macaca mulatta</topic><topic>Torque teno virus - genetics</topic><topic>Torque teno virus - isolation & purification</topic><topic>Torque teno virus - physiology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiao, Hong</creatorcontrib><creatorcontrib>Luo, Kangxian</creatorcontrib><creatorcontrib>Yang, Shouchang</creatorcontrib><creatorcontrib>Wang, Yanjun</creatorcontrib><creatorcontrib>Liang, Weifang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiao, Hong</au><au>Luo, Kangxian</au><au>Yang, Shouchang</au><au>Wang, Yanjun</au><au>Liang, Weifang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue tropism of the TTV in experimentally infected rhesus monkeys</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chin Med J (Engl)</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>115</volume><issue>7</issue><spage>1088</spage><epage>1090</epage><pages>1088-1090</pages><issn>0366-6999</issn><abstract>To determine whether the transfusion-transmitted virus (TTV) is hepatotropic.
Total DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes.
The double-stranded probe was hybridized with DNA from the liver, bone marrow, spleen,stomach, small intestine and colon. The single-stranded antisense probe was hybridized with DNA from the liver, small intestine and bone marrow of all 5 monkeys, but not with that from other tissues.
As the viral genome was of negative polarity, the plus-stranded fragment identified in our study might be a replicative intermediate, and was only demonstrated in the liver, small intestine, and bone marrow by dot blot hybridization with single-stranded antisense probes. It is suggested that the TTV replicates in the liver, bone marrow and small intestine, and TTV might be hepatotropic.</abstract><cop>China</cop><pmid>12173599</pmid><tpages>3</tpages></addata></record> |
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| ispartof | Chinese medical journal, 2002-07, Vol.115 (7), p.1088-1090 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Bone Marrow - virology DNA Virus Infections - virology DNA, Viral - analysis Intestine, Small - virology Liver - virology Macaca mulatta Torque teno virus - genetics Torque teno virus - isolation & purification Torque teno virus - physiology Virus Replication |
| title | Tissue tropism of the TTV in experimentally infected rhesus monkeys |
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