Improving 1,3-propanediol production from glycerol in a metabolically engineered Escherichia coli by reducing accumulation of sn-glycerol-3-phosphate

High levels of glycerol significantly inhibit cell growth and 1,3-propanediol (1,3-PD) production in anaerobic glycerol fermentation by genetically engineered Escherichia coli (E. coli) strains expressing genes from the Klebsiella pneumoniae dha (K.pneumoniae) regulon. We have previously demonstrate...

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Veröffentlicht in:	Biotechnology progress 2002-07, Vol.18 (4), p.694-699
Hauptverfasser:	ZHU, Marie M, LAWMAN, Patricia D, CAMERON, Douglas C
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaerobiosis Biological and medical sciences Biotechnology Culture Media Enzyme Induction Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - metabolism Fermentation Fundamental and applied biological sciences. Psychology Gene Expression Genes, Bacterial Genetic Engineering Glycerol - metabolism Glycerol Kinase - deficiency Glycerol Kinase - genetics Glycerol Kinase - metabolism Glycerolphosphate Dehydrogenase - metabolism Glycerophosphates - metabolism Klebsiella pneumoniae - metabolism Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Mutation Propylene Glycols - metabolism
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