Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA
We obtained 7,566 peripheral blood mononuclear cell (PBMC) samples from 2,332 individuals and screened them for human herpesvirus infection. We identified five individuals who persistently harbored high copy numbers of human herpesvirus 6 (HHV‐6) DNA in their PBMCs. HHV‐6 DNA was also detected in ot...
Gespeichert in:
| Veröffentlicht in: | Journal of medical virology 2004-07, Vol.73 (3), p.465-473 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 473 |
|---|---|
| container_issue | 3 |
| container_start_page | 465 |
| container_title | Journal of medical virology |
| container_volume | 73 |
| creator | Tanaka-Taya, Keiko Sashihara, Junji Kurahashi, Hiroki Amo, Kiyoko Miyagawa, Hiromi Kondo, Kazuhiro Okada, Shintaro Yamanishi, Koichi |
| description | We obtained 7,566 peripheral blood mononuclear cell (PBMC) samples from 2,332 individuals and screened them for human herpesvirus infection. We identified five individuals who persistently harbored high copy numbers of human herpesvirus 6 (HHV‐6) DNA in their PBMCs. HHV‐6 DNA was also detected in other somatic tissues of these individuals. Five additional cases were identified among their family members. For two of these families, chromosomally integrated HHV‐6 DNA (CIHHV‐6) was detected in the PBMCs by fluorescence in situ hybridization. The prevalence of CIHHV‐6 among all the subjects was 0.21%. The HHV‐6 DNA was variant B in four families and variant A in one family. Antibodies to immediate early antigen and glycoprotein B were detected in 57 and 14% of individuals with CIHHV‐6 and in 0 and 60% of healthy volunteers without CIHHV‐6, respectively. HHV‐6 could not be isolated from PBMCs with CIHHV‐6. These cases shared no clinical features, and included three healthy individuals. Our data suggest that CIHHV‐6 is rare but detectable in the general population and that hereditary transmission is one of the routes of HHV‐6 transmission. J. Med. Virol. 73:465–473, 2004. © 2004 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jmv.20113 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71967770</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71967770</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4203-89603b0774cbb1ae1d24df48af9f549ac1b4c1691ee1f66b53698f28164cab0f3</originalsourceid><addsrcrecordid>eNqFkcFu1DAURSMEokNhwQ8gb6jaRVq_xLHjZVVKA7RlU6YSG8txbMYliQfbaRl-hZ_FnRmgG8TKkt-59y5Olr0EfAgYF0c3w-1hgQHKR9kMMKc5xwweZzMMhOaUQrWTPQvhBmNc86J4mu1ABQxTQmbZz2Ya5IgW2i91uLV-Coii_aaZ5_QA2YCil2MYbIy6Q8a7AS2l12NE0SG1sH2H7IhS3o5Rf_FyTTk_pK8u3aWXKmpvf8ho3YicQUoGHdCdjYt0TnUuuEH2_ephwXocvbk8fp49MbIP-sX23c0-vT29Omny849n706Oz3NFClzmNae4bDFjRLUtSA1dQTpDamm4qQiXClqigHLQGgylbVVSXpuiBkqUbLEpd7O9Te_Su2-TDlEMNijd93LUbgqCAaeMMfxfEBinnJIqgQcbUHkXgtdGLL0dpF8JwOJemUjKxFpZYl9tS6d20N1fcusoAa-3gAxK9iYZUTY84BirGb8fPdpwd7bXq38vivcX89_T-SZhQ9Tf_ySk_yooK1klri_PxAVcf54XzQdxVf4CHIi9IQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17969645</pqid></control><display><type>article</type><title>Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Tanaka-Taya, Keiko ; Sashihara, Junji ; Kurahashi, Hiroki ; Amo, Kiyoko ; Miyagawa, Hiromi ; Kondo, Kazuhiro ; Okada, Shintaro ; Yamanishi, Koichi</creator><creatorcontrib>Tanaka-Taya, Keiko ; Sashihara, Junji ; Kurahashi, Hiroki ; Amo, Kiyoko ; Miyagawa, Hiromi ; Kondo, Kazuhiro ; Okada, Shintaro ; Yamanishi, Koichi</creatorcontrib><description>We obtained 7,566 peripheral blood mononuclear cell (PBMC) samples from 2,332 individuals and screened them for human herpesvirus infection. We identified five individuals who persistently harbored high copy numbers of human herpesvirus 6 (HHV‐6) DNA in their PBMCs. HHV‐6 DNA was also detected in other somatic tissues of these individuals. Five additional cases were identified among their family members. For two of these families, chromosomally integrated HHV‐6 DNA (CIHHV‐6) was detected in the PBMCs by fluorescence in situ hybridization. The prevalence of CIHHV‐6 among all the subjects was 0.21%. The HHV‐6 DNA was variant B in four families and variant A in one family. Antibodies to immediate early antigen and glycoprotein B were detected in 57 and 14% of individuals with CIHHV‐6 and in 0 and 60% of healthy volunteers without CIHHV‐6, respectively. HHV‐6 could not be isolated from PBMCs with CIHHV‐6. These cases shared no clinical features, and included three healthy individuals. Our data suggest that CIHHV‐6 is rare but detectable in the general population and that hereditary transmission is one of the routes of HHV‐6 transmission. J. Med. Virol. 73:465–473, 2004. © 2004 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.20113</identifier><identifier>PMID: 15170644</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Antibodies, Viral - blood ; Biological and medical sciences ; Chromosomes, Human - virology ; CIHHV-6 ; DNA, Viral - analysis ; Female ; FISH ; Fundamental and applied biological sciences. Psychology ; glycoprotein B ; Hair - virology ; hereditary transmission ; Herpesvirus 6, Human - genetics ; Herpesvirus 6, Human - isolation & purification ; Human herpesvirus 6 ; Human viral diseases ; Humans ; immediate early ; Immediate-Early Proteins - immunology ; In Situ Hybridization, Fluorescence ; Infectious Disease Transmission, Vertical ; Infectious diseases ; Leukocytes, Mononuclear - virology ; Male ; Medical sciences ; Microbiology ; Miscellaneous ; Mouth Mucosa - virology ; Pedigree ; Pharynx - virology ; Phosphoproteins - immunology ; Polymerase Chain Reaction ; Roseolovirus Infections - transmission ; Roseolovirus Infections - virology ; Viral diseases ; Viral Envelope Proteins - immunology ; Virology ; Virus Integration</subject><ispartof>Journal of medical virology, 2004-07, Vol.73 (3), p.465-473</ispartof><rights>Copyright © 2004 Wiley‐Liss, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4203-89603b0774cbb1ae1d24df48af9f549ac1b4c1691ee1f66b53698f28164cab0f3</citedby><cites>FETCH-LOGICAL-c4203-89603b0774cbb1ae1d24df48af9f549ac1b4c1691ee1f66b53698f28164cab0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.20113$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.20113$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15778795$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15170644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tanaka-Taya, Keiko</creatorcontrib><creatorcontrib>Sashihara, Junji</creatorcontrib><creatorcontrib>Kurahashi, Hiroki</creatorcontrib><creatorcontrib>Amo, Kiyoko</creatorcontrib><creatorcontrib>Miyagawa, Hiromi</creatorcontrib><creatorcontrib>Kondo, Kazuhiro</creatorcontrib><creatorcontrib>Okada, Shintaro</creatorcontrib><creatorcontrib>Yamanishi, Koichi</creatorcontrib><title>Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>We obtained 7,566 peripheral blood mononuclear cell (PBMC) samples from 2,332 individuals and screened them for human herpesvirus infection. We identified five individuals who persistently harbored high copy numbers of human herpesvirus 6 (HHV‐6) DNA in their PBMCs. HHV‐6 DNA was also detected in other somatic tissues of these individuals. Five additional cases were identified among their family members. For two of these families, chromosomally integrated HHV‐6 DNA (CIHHV‐6) was detected in the PBMCs by fluorescence in situ hybridization. The prevalence of CIHHV‐6 among all the subjects was 0.21%. The HHV‐6 DNA was variant B in four families and variant A in one family. Antibodies to immediate early antigen and glycoprotein B were detected in 57 and 14% of individuals with CIHHV‐6 and in 0 and 60% of healthy volunteers without CIHHV‐6, respectively. HHV‐6 could not be isolated from PBMCs with CIHHV‐6. These cases shared no clinical features, and included three healthy individuals. Our data suggest that CIHHV‐6 is rare but detectable in the general population and that hereditary transmission is one of the routes of HHV‐6 transmission. J. Med. Virol. 73:465–473, 2004. © 2004 Wiley‐Liss, Inc.</description><subject>Antibodies, Viral - blood</subject><subject>Biological and medical sciences</subject><subject>Chromosomes, Human - virology</subject><subject>CIHHV-6</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>FISH</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glycoprotein B</subject><subject>Hair - virology</subject><subject>hereditary transmission</subject><subject>Herpesvirus 6, Human - genetics</subject><subject>Herpesvirus 6, Human - isolation & purification</subject><subject>Human herpesvirus 6</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>immediate early</subject><subject>Immediate-Early Proteins - immunology</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Infectious Disease Transmission, Vertical</subject><subject>Infectious diseases</subject><subject>Leukocytes, Mononuclear - virology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mouth Mucosa - virology</subject><subject>Pedigree</subject><subject>Pharynx - virology</subject><subject>Phosphoproteins - immunology</subject><subject>Polymerase Chain Reaction</subject><subject>Roseolovirus Infections - transmission</subject><subject>Roseolovirus Infections - virology</subject><subject>Viral diseases</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><subject>Virus Integration</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURSMEokNhwQ8gb6jaRVq_xLHjZVVKA7RlU6YSG8txbMYliQfbaRl-hZ_FnRmgG8TKkt-59y5Olr0EfAgYF0c3w-1hgQHKR9kMMKc5xwweZzMMhOaUQrWTPQvhBmNc86J4mu1ABQxTQmbZz2Ya5IgW2i91uLV-Coii_aaZ5_QA2YCil2MYbIy6Q8a7AS2l12NE0SG1sH2H7IhS3o5Rf_FyTTk_pK8u3aWXKmpvf8ho3YicQUoGHdCdjYt0TnUuuEH2_ephwXocvbk8fp49MbIP-sX23c0-vT29Omny849n706Oz3NFClzmNae4bDFjRLUtSA1dQTpDamm4qQiXClqigHLQGgylbVVSXpuiBkqUbLEpd7O9Te_Su2-TDlEMNijd93LUbgqCAaeMMfxfEBinnJIqgQcbUHkXgtdGLL0dpF8JwOJemUjKxFpZYl9tS6d20N1fcusoAa-3gAxK9iYZUTY84BirGb8fPdpwd7bXq38vivcX89_T-SZhQ9Tf_ySk_yooK1klri_PxAVcf54XzQdxVf4CHIi9IQ</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Tanaka-Taya, Keiko</creator><creator>Sashihara, Junji</creator><creator>Kurahashi, Hiroki</creator><creator>Amo, Kiyoko</creator><creator>Miyagawa, Hiromi</creator><creator>Kondo, Kazuhiro</creator><creator>Okada, Shintaro</creator><creator>Yamanishi, Koichi</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA</title><author>Tanaka-Taya, Keiko ; Sashihara, Junji ; Kurahashi, Hiroki ; Amo, Kiyoko ; Miyagawa, Hiromi ; Kondo, Kazuhiro ; Okada, Shintaro ; Yamanishi, Koichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4203-89603b0774cbb1ae1d24df48af9f549ac1b4c1691ee1f66b53698f28164cab0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Antibodies, Viral - blood</topic><topic>Biological and medical sciences</topic><topic>Chromosomes, Human - virology</topic><topic>CIHHV-6</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>FISH</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glycoprotein B</topic><topic>Hair - virology</topic><topic>hereditary transmission</topic><topic>Herpesvirus 6, Human - genetics</topic><topic>Herpesvirus 6, Human - isolation & purification</topic><topic>Human herpesvirus 6</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>immediate early</topic><topic>Immediate-Early Proteins - immunology</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Infectious Disease Transmission, Vertical</topic><topic>Infectious diseases</topic><topic>Leukocytes, Mononuclear - virology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mouth Mucosa - virology</topic><topic>Pedigree</topic><topic>Pharynx - virology</topic><topic>Phosphoproteins - immunology</topic><topic>Polymerase Chain Reaction</topic><topic>Roseolovirus Infections - transmission</topic><topic>Roseolovirus Infections - virology</topic><topic>Viral diseases</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><topic>Virus Integration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanaka-Taya, Keiko</creatorcontrib><creatorcontrib>Sashihara, Junji</creatorcontrib><creatorcontrib>Kurahashi, Hiroki</creatorcontrib><creatorcontrib>Amo, Kiyoko</creatorcontrib><creatorcontrib>Miyagawa, Hiromi</creatorcontrib><creatorcontrib>Kondo, Kazuhiro</creatorcontrib><creatorcontrib>Okada, Shintaro</creatorcontrib><creatorcontrib>Yamanishi, Koichi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanaka-Taya, Keiko</au><au>Sashihara, Junji</au><au>Kurahashi, Hiroki</au><au>Amo, Kiyoko</au><au>Miyagawa, Hiromi</au><au>Kondo, Kazuhiro</au><au>Okada, Shintaro</au><au>Yamanishi, Koichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2004-07</date><risdate>2004</risdate><volume>73</volume><issue>3</issue><spage>465</spage><epage>473</epage><pages>465-473</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>We obtained 7,566 peripheral blood mononuclear cell (PBMC) samples from 2,332 individuals and screened them for human herpesvirus infection. We identified five individuals who persistently harbored high copy numbers of human herpesvirus 6 (HHV‐6) DNA in their PBMCs. HHV‐6 DNA was also detected in other somatic tissues of these individuals. Five additional cases were identified among their family members. For two of these families, chromosomally integrated HHV‐6 DNA (CIHHV‐6) was detected in the PBMCs by fluorescence in situ hybridization. The prevalence of CIHHV‐6 among all the subjects was 0.21%. The HHV‐6 DNA was variant B in four families and variant A in one family. Antibodies to immediate early antigen and glycoprotein B were detected in 57 and 14% of individuals with CIHHV‐6 and in 0 and 60% of healthy volunteers without CIHHV‐6, respectively. HHV‐6 could not be isolated from PBMCs with CIHHV‐6. These cases shared no clinical features, and included three healthy individuals. Our data suggest that CIHHV‐6 is rare but detectable in the general population and that hereditary transmission is one of the routes of HHV‐6 transmission. J. Med. Virol. 73:465–473, 2004. © 2004 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15170644</pmid><doi>10.1002/jmv.20113</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-6615 |
| ispartof | Journal of medical virology, 2004-07, Vol.73 (3), p.465-473 |
| issn | 0146-6615 1096-9071 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71967770 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antibodies, Viral - blood Biological and medical sciences Chromosomes, Human - virology CIHHV-6 DNA, Viral - analysis Female FISH Fundamental and applied biological sciences. Psychology glycoprotein B Hair - virology hereditary transmission Herpesvirus 6, Human - genetics Herpesvirus 6, Human - isolation & purification Human herpesvirus 6 Human viral diseases Humans immediate early Immediate-Early Proteins - immunology In Situ Hybridization, Fluorescence Infectious Disease Transmission, Vertical Infectious diseases Leukocytes, Mononuclear - virology Male Medical sciences Microbiology Miscellaneous Mouth Mucosa - virology Pedigree Pharynx - virology Phosphoproteins - immunology Polymerase Chain Reaction Roseolovirus Infections - transmission Roseolovirus Infections - virology Viral diseases Viral Envelope Proteins - immunology Virology Virus Integration |
| title | Human herpesvirus 6 (HHV-6) is transmitted from parent to child in an integrated form and characterization of cases with chromosomally integrated HHV-6 DNA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T21%3A08%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20herpesvirus%206%20(HHV-6)%20is%20transmitted%20from%20parent%20to%20child%20in%20an%20integrated%20form%20and%20characterization%20of%20cases%20with%20chromosomally%20integrated%20HHV-6%20DNA&rft.jtitle=Journal%20of%20medical%20virology&rft.au=Tanaka-Taya,%20Keiko&rft.date=2004-07&rft.volume=73&rft.issue=3&rft.spage=465&rft.epage=473&rft.pages=465-473&rft.issn=0146-6615&rft.eissn=1096-9071&rft.coden=JMVIDB&rft_id=info:doi/10.1002/jmv.20113&rft_dat=%3Cproquest_cross%3E71967770%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17969645&rft_id=info:pmid/15170644&rfr_iscdi=true |