Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination
Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce AtRAD17 transcr...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2004-06, Vol.38 (6), p.954-968 |
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| creator | Heitzeberg, F Chen, I.P Hartung, F Orel, N Angelis, K.J Puchta, H |
| description | Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce AtRAD17 transcriptionally. AtRAD17 mutants show increased sensitivity to the DNA-damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9-1-1 complex (Rad9), which is also sensitive to the same chemicals. AtRAD9 and AtRAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double-strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants are proficient for a further induction of HR by genotoxic stresses. Our results indicate that a mutant Rad17 pathway is associated with a general deregulation of DNA repair, which seems to be correlated with a deficiency in non-homologous DSB repair. |
| doi_str_mv | 10.1111/j.1365-313x.2004.02097.x |
| format | Article |
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A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce AtRAD17 transcriptionally. AtRAD17 mutants show increased sensitivity to the DNA-damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9-1-1 complex (Rad9), which is also sensitive to the same chemicals. AtRAD9 and AtRAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double-strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants are proficient for a further induction of HR by genotoxic stresses. Our results indicate that a mutant Rad17 pathway is associated with a general deregulation of DNA repair, which seems to be correlated with a deficiency in non-homologous DSB repair.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2004.02097.x</identifier><identifier>PMID: 15165187</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis Proteins ; Arabidopsis RAD17 protein ; Arabidopsis thaliana ; Biological and medical sciences ; Cell Cycle Proteins - chemistry ; Cell Cycle Proteins - isolation & purification ; Cell Cycle Proteins - physiology ; comet assay ; complementary DNA ; DNA damage ; DNA Damage - physiology ; DNA repair ; DNA Repair - physiology ; DNA, Complementary - analysis ; DNA-Binding Proteins ; double‐strand break repair ; Endonucleases - isolation & purification ; exodeoxyribonucleases ; Fundamental and applied biological sciences. Psychology ; genetic complementation ; genetic recombination ; intrachromosomal homologous recombination ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis. Repair ; mutagens ; mutants ; nucleotide sequences ; plant proteins ; RAD17 ; RAD9 ; Recombination, Genetic ; Schizosaccharomyces pombe Proteins ; sequence analysis ; Sequence Homology, Amino Acid</subject><ispartof>The Plant journal : for cell and molecular biology, 2004-06, Vol.38 (6), p.954-968</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5667-c000824747ded04080428fb3474ddafd1e348059044bd37e9a18c3a60c49ad8f3</citedby><cites>FETCH-LOGICAL-c5667-c000824747ded04080428fb3474ddafd1e348059044bd37e9a18c3a60c49ad8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2004.02097.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2004.02097.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,1435,27931,27932,45581,45582,46416,46840</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15778263$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15165187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heitzeberg, F</creatorcontrib><creatorcontrib>Chen, I.P</creatorcontrib><creatorcontrib>Hartung, F</creatorcontrib><creatorcontrib>Orel, N</creatorcontrib><creatorcontrib>Angelis, K.J</creatorcontrib><creatorcontrib>Puchta, H</creatorcontrib><title>Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce AtRAD17 transcriptionally. AtRAD17 mutants show increased sensitivity to the DNA-damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9-1-1 complex (Rad9), which is also sensitive to the same chemicals. AtRAD9 and AtRAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double-strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants are proficient for a further induction of HR by genotoxic stresses. Our results indicate that a mutant Rad17 pathway is associated with a general deregulation of DNA repair, which seems to be correlated with a deficiency in non-homologous DSB repair.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins</subject><subject>Arabidopsis RAD17 protein</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle Proteins - chemistry</subject><subject>Cell Cycle Proteins - isolation & purification</subject><subject>Cell Cycle Proteins - physiology</subject><subject>comet assay</subject><subject>complementary DNA</subject><subject>DNA damage</subject><subject>DNA Damage - physiology</subject><subject>DNA repair</subject><subject>DNA Repair - physiology</subject><subject>DNA, Complementary - analysis</subject><subject>DNA-Binding Proteins</subject><subject>double‐strand break repair</subject><subject>Endonucleases - isolation & purification</subject><subject>exodeoxyribonucleases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic complementation</subject><subject>genetic recombination</subject><subject>intrachromosomal homologous recombination</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis. Repair</subject><subject>mutagens</subject><subject>mutants</subject><subject>nucleotide sequences</subject><subject>plant proteins</subject><subject>RAD17</subject><subject>RAD9</subject><subject>Recombination, Genetic</subject><subject>Schizosaccharomyces pombe Proteins</subject><subject>sequence analysis</subject><subject>Sequence Homology, Amino Acid</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi1ERZfCX4Bc4JYwjh1_HDisyreqgqCVuFmT2NlmlcRbe1O2_x6nu1W5FcuSR-PnnRnNS0hGoaDpvFsXlIkqZ5TtihKAF1CClsXuCVncf_x-ShagBeSS0_KYPI9xDUAlE_wZOaYVFRVVckHGn2ipzK784Hu_mlzm22wZsO6s38QuZvMdb3x_42wKsu2Vy4JbTT1uOz_O8IfzZWZxwNX8scEuZDja-3p-iinb-KHuxjvFC3LUYh_dy8N7Qi4_fbw4_ZKfff_89XR5ljeVEDJvAECVXHJpnQUOCnip2pqljLXYWuoYV1Bp4Ly2TDqNVDUMBTRco1UtOyFv93U3wV9PLm7N0MXG9T2OLg1lJNWC8Uo_ClKpteZKJFDtwSb4GINrzSZ0A4ZbQ8HMppi1mXdv5t2b2RRzZ4rZJemrQ4-pHpx9EB5cSMCbA4Cxwb4NODZd_IeTUpWCJe79nvvT9e72vwcwFz--zVHSv97rW_QGVyH1uPxVAmUAmnGd1vEXTDmxWQ</recordid><startdate>200406</startdate><enddate>200406</enddate><creator>Heitzeberg, F</creator><creator>Chen, I.P</creator><creator>Hartung, F</creator><creator>Orel, N</creator><creator>Angelis, K.J</creator><creator>Puchta, H</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200406</creationdate><title>Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination</title><author>Heitzeberg, F ; Chen, I.P ; Hartung, F ; Orel, N ; Angelis, K.J ; Puchta, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5667-c000824747ded04080428fb3474ddafd1e348059044bd37e9a18c3a60c49ad8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins</topic><topic>Arabidopsis RAD17 protein</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle Proteins - chemistry</topic><topic>Cell Cycle Proteins - isolation & purification</topic><topic>Cell Cycle Proteins - physiology</topic><topic>comet assay</topic><topic>complementary DNA</topic><topic>DNA damage</topic><topic>DNA Damage - physiology</topic><topic>DNA repair</topic><topic>DNA Repair - physiology</topic><topic>DNA, Complementary - analysis</topic><topic>DNA-Binding Proteins</topic><topic>double‐strand break repair</topic><topic>Endonucleases - isolation & purification</topic><topic>exodeoxyribonucleases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic complementation</topic><topic>genetic recombination</topic><topic>intrachromosomal homologous recombination</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis. Repair</topic><topic>mutagens</topic><topic>mutants</topic><topic>nucleotide sequences</topic><topic>plant proteins</topic><topic>RAD17</topic><topic>RAD9</topic><topic>Recombination, Genetic</topic><topic>Schizosaccharomyces pombe Proteins</topic><topic>sequence analysis</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heitzeberg, F</creatorcontrib><creatorcontrib>Chen, I.P</creatorcontrib><creatorcontrib>Hartung, F</creatorcontrib><creatorcontrib>Orel, N</creatorcontrib><creatorcontrib>Angelis, K.J</creatorcontrib><creatorcontrib>Puchta, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heitzeberg, F</au><au>Chen, I.P</au><au>Hartung, F</au><au>Orel, N</au><au>Angelis, K.J</au><au>Puchta, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2004-06</date><risdate>2004</risdate><volume>38</volume><issue>6</issue><spage>954</spage><epage>968</epage><pages>954-968</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce AtRAD17 transcriptionally. AtRAD17 mutants show increased sensitivity to the DNA-damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9-1-1 complex (Rad9), which is also sensitive to the same chemicals. AtRAD9 and AtRAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double-strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants are proficient for a further induction of HR by genotoxic stresses. Our results indicate that a mutant Rad17 pathway is associated with a general deregulation of DNA repair, which seems to be correlated with a deficiency in non-homologous DSB repair.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15165187</pmid><doi>10.1111/j.1365-313x.2004.02097.x</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Arabidopsis Arabidopsis - genetics Arabidopsis Proteins Arabidopsis RAD17 protein Arabidopsis thaliana Biological and medical sciences Cell Cycle Proteins - chemistry Cell Cycle Proteins - isolation & purification Cell Cycle Proteins - physiology comet assay complementary DNA DNA damage DNA Damage - physiology DNA repair DNA Repair - physiology DNA, Complementary - analysis DNA-Binding Proteins double‐strand break repair Endonucleases - isolation & purification exodeoxyribonucleases Fundamental and applied biological sciences. Psychology genetic complementation genetic recombination intrachromosomal homologous recombination Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis. Repair mutagens mutants nucleotide sequences plant proteins RAD17 RAD9 Recombination, Genetic Schizosaccharomyces pombe Proteins sequence analysis Sequence Homology, Amino Acid |
| title | Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination |
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