Grape Seed Extract Affects Proliferation and Differentiation of Human Intestinal Caco-2 Cells
The effect of daily contact of a grape seed extract (GSE) on Caco-2 cell proliferation and differentiation was investigated. GSE at 400 mg/L was added to Caco-2 cells for 2 h a day after successive incubation in saliva, gastric, and pancreatic media. When applied at the beginning of the cell culture...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2004-06, Vol.52 (11), p.3301-3308 |
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| creator | Laurent, Caroline Besançon, Pierre Auger, Cyril Rouanet, Jean-Max Caporiccio, Bertrand |
| description | The effect of daily contact of a grape seed extract (GSE) on Caco-2 cell proliferation and differentiation was investigated. GSE at 400 mg/L was added to Caco-2 cells for 2 h a day after successive incubation in saliva, gastric, and pancreatic media. When applied at the beginning of the cell culture, GSE triggered inhibition of cell growth associated with a possible cytotoxic reaction. On the other hand, when the treatment was applied to confluent cells, treated cells displayed a higher protein content than control cells and a more developed brush border, with taller and denser microvilli. These observations were accompanied by stimulation of alkaline phosphatase activity, especially at day 5 postconfluency, with a 2.2-fold increase in comparison with the control. On the other hand, aminopeptidase N activity was inhibited throughout the differentiation period in GSE-treated cells to reach 28.8% of control cell activity on day 30. GSE did not affect either sucrase−isomaltase activity or cytoplasmic lactate dehydrogenase (LDH) activity, which otherwise appeared to be a good cellular marker. GSE treatment of Caco-2 cells thus inhibited their proliferation from seeding onward and stimulated both proliferation and differentiation after confluency. Keywords: Caco-2 cells; phenolic compounds; differentiation; proliferation |
| doi_str_mv | 10.1021/jf035231e |
| format | Article |
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GSE at 400 mg/L was added to Caco-2 cells for 2 h a day after successive incubation in saliva, gastric, and pancreatic media. When applied at the beginning of the cell culture, GSE triggered inhibition of cell growth associated with a possible cytotoxic reaction. On the other hand, when the treatment was applied to confluent cells, treated cells displayed a higher protein content than control cells and a more developed brush border, with taller and denser microvilli. These observations were accompanied by stimulation of alkaline phosphatase activity, especially at day 5 postconfluency, with a 2.2-fold increase in comparison with the control. On the other hand, aminopeptidase N activity was inhibited throughout the differentiation period in GSE-treated cells to reach 28.8% of control cell activity on day 30. GSE did not affect either sucrase−isomaltase activity or cytoplasmic lactate dehydrogenase (LDH) activity, which otherwise appeared to be a good cellular marker. GSE treatment of Caco-2 cells thus inhibited their proliferation from seeding onward and stimulated both proliferation and differentiation after confluency. Keywords: Caco-2 cells; phenolic compounds; differentiation; proliferation</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf035231e</identifier><identifier>PMID: 15161187</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>alkaline phosphatase ; aminopeptidases ; Biological and medical sciences ; brush border membrane vesicles ; Caco-2 Cells ; cell differentiation ; Cell Differentiation - drug effects ; Cell Division - drug effects ; cell lines ; cell proliferation ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; grape seed extract ; Humans ; intestinal mucosa ; Intestine, Small - cytology ; Intestine. Mesentery ; medicinal properties ; Microscopy, Electron ; microvilli ; Plant Extracts - pharmacology ; Seeds - chemistry ; sucrose alpha-glucosidase ; Vertebrates: digestive system ; Vitis - chemistry</subject><ispartof>Journal of agricultural and food chemistry, 2004-06, Vol.52 (11), p.3301-3308</ispartof><rights>Copyright © 2004 American Chemical Society</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a469t-48be063efcfcd120f04065f9ebc2d35376c7bb48e4b1f86b315f5611545e18c3</citedby><cites>FETCH-LOGICAL-a469t-48be063efcfcd120f04065f9ebc2d35376c7bb48e4b1f86b315f5611545e18c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf035231e$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf035231e$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15779831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15161187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laurent, Caroline</creatorcontrib><creatorcontrib>Besançon, Pierre</creatorcontrib><creatorcontrib>Auger, Cyril</creatorcontrib><creatorcontrib>Rouanet, Jean-Max</creatorcontrib><creatorcontrib>Caporiccio, Bertrand</creatorcontrib><title>Grape Seed Extract Affects Proliferation and Differentiation of Human Intestinal Caco-2 Cells</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The effect of daily contact of a grape seed extract (GSE) on Caco-2 cell proliferation and differentiation was investigated. GSE at 400 mg/L was added to Caco-2 cells for 2 h a day after successive incubation in saliva, gastric, and pancreatic media. When applied at the beginning of the cell culture, GSE triggered inhibition of cell growth associated with a possible cytotoxic reaction. On the other hand, when the treatment was applied to confluent cells, treated cells displayed a higher protein content than control cells and a more developed brush border, with taller and denser microvilli. These observations were accompanied by stimulation of alkaline phosphatase activity, especially at day 5 postconfluency, with a 2.2-fold increase in comparison with the control. On the other hand, aminopeptidase N activity was inhibited throughout the differentiation period in GSE-treated cells to reach 28.8% of control cell activity on day 30. GSE did not affect either sucrase−isomaltase activity or cytoplasmic lactate dehydrogenase (LDH) activity, which otherwise appeared to be a good cellular marker. GSE treatment of Caco-2 cells thus inhibited their proliferation from seeding onward and stimulated both proliferation and differentiation after confluency. Keywords: Caco-2 cells; phenolic compounds; differentiation; proliferation</description><subject>alkaline phosphatase</subject><subject>aminopeptidases</subject><subject>Biological and medical sciences</subject><subject>brush border membrane vesicles</subject><subject>Caco-2 Cells</subject><subject>cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>cell lines</subject><subject>cell proliferation</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>grape seed extract</subject><subject>Humans</subject><subject>intestinal mucosa</subject><subject>Intestine, Small - cytology</subject><subject>Intestine. Mesentery</subject><subject>medicinal properties</subject><subject>Microscopy, Electron</subject><subject>microvilli</subject><subject>Plant Extracts - pharmacology</subject><subject>Seeds - chemistry</subject><subject>sucrose alpha-glucosidase</subject><subject>Vertebrates: digestive system</subject><subject>Vitis - chemistry</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E1v1DAQBmALgei2cOAPgC9U4hAYx_FHjlUouxWVqLTLBQlZjjNGWbLxYidS-fcYZdVy4GRp5tF45iXkFYP3DEr2Ye-Bi5IzfEJWTJRQCMb0U7KC3Cy0kOyMnKe0BwAtFDwnZ0wwmYlake_raI9It4gdvb6fonUTvfIe3ZToXQxD7zHaqQ8jtWNHP_a5FXGc-qUWPN3MBzvSm3HCNPWjHWhjXShK2uAwpBfkmbdDwpen94LsPl3vmk1x-2V901zdFraS9VRUukWQHL3zrmMleKhACl9j68qOC66kU21baaxa5rVsORM-X8VEJZBpxy_I5TL2GMOvOS9iDn1yeQE7YpiTUayWoEBn-G6BLoaUInpzjP3Bxt-GgfkbpXmIMtvXp6Fze8DuUZ6yy-DtCdjk7OCjHV2f_nFK1Zqz7IrF9WnC-4e-jT-NVFwJs7vbGi2_rTeft9o02b9ZvLfB2B8xz_y6LYFxgJrXWovHn61LZh_mmHNP_znhD_Pfntk</recordid><startdate>20040602</startdate><enddate>20040602</enddate><creator>Laurent, Caroline</creator><creator>Besançon, Pierre</creator><creator>Auger, Cyril</creator><creator>Rouanet, Jean-Max</creator><creator>Caporiccio, Bertrand</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040602</creationdate><title>Grape Seed Extract Affects Proliferation and Differentiation of Human Intestinal Caco-2 Cells</title><author>Laurent, Caroline ; Besançon, Pierre ; Auger, Cyril ; Rouanet, Jean-Max ; Caporiccio, Bertrand</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a469t-48be063efcfcd120f04065f9ebc2d35376c7bb48e4b1f86b315f5611545e18c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>alkaline phosphatase</topic><topic>aminopeptidases</topic><topic>Biological and medical sciences</topic><topic>brush border membrane vesicles</topic><topic>Caco-2 Cells</topic><topic>cell differentiation</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>cell lines</topic><topic>cell proliferation</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>grape seed extract</topic><topic>Humans</topic><topic>intestinal mucosa</topic><topic>Intestine, Small - cytology</topic><topic>Intestine. Mesentery</topic><topic>medicinal properties</topic><topic>Microscopy, Electron</topic><topic>microvilli</topic><topic>Plant Extracts - pharmacology</topic><topic>Seeds - chemistry</topic><topic>sucrose alpha-glucosidase</topic><topic>Vertebrates: digestive system</topic><topic>Vitis - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laurent, Caroline</creatorcontrib><creatorcontrib>Besançon, Pierre</creatorcontrib><creatorcontrib>Auger, Cyril</creatorcontrib><creatorcontrib>Rouanet, Jean-Max</creatorcontrib><creatorcontrib>Caporiccio, Bertrand</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laurent, Caroline</au><au>Besançon, Pierre</au><au>Auger, Cyril</au><au>Rouanet, Jean-Max</au><au>Caporiccio, Bertrand</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Grape Seed Extract Affects Proliferation and Differentiation of Human Intestinal Caco-2 Cells</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2004-06-02</date><risdate>2004</risdate><volume>52</volume><issue>11</issue><spage>3301</spage><epage>3308</epage><pages>3301-3308</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The effect of daily contact of a grape seed extract (GSE) on Caco-2 cell proliferation and differentiation was investigated. GSE at 400 mg/L was added to Caco-2 cells for 2 h a day after successive incubation in saliva, gastric, and pancreatic media. When applied at the beginning of the cell culture, GSE triggered inhibition of cell growth associated with a possible cytotoxic reaction. On the other hand, when the treatment was applied to confluent cells, treated cells displayed a higher protein content than control cells and a more developed brush border, with taller and denser microvilli. These observations were accompanied by stimulation of alkaline phosphatase activity, especially at day 5 postconfluency, with a 2.2-fold increase in comparison with the control. On the other hand, aminopeptidase N activity was inhibited throughout the differentiation period in GSE-treated cells to reach 28.8% of control cell activity on day 30. GSE did not affect either sucrase−isomaltase activity or cytoplasmic lactate dehydrogenase (LDH) activity, which otherwise appeared to be a good cellular marker. GSE treatment of Caco-2 cells thus inhibited their proliferation from seeding onward and stimulated both proliferation and differentiation after confluency. Keywords: Caco-2 cells; phenolic compounds; differentiation; proliferation</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15161187</pmid><doi>10.1021/jf035231e</doi><tpages>8</tpages></addata></record> |
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| subjects | alkaline phosphatase aminopeptidases Biological and medical sciences brush border membrane vesicles Caco-2 Cells cell differentiation Cell Differentiation - drug effects Cell Division - drug effects cell lines cell proliferation enzyme activity Fundamental and applied biological sciences. Psychology grape seed extract Humans intestinal mucosa Intestine, Small - cytology Intestine. Mesentery medicinal properties Microscopy, Electron microvilli Plant Extracts - pharmacology Seeds - chemistry sucrose alpha-glucosidase Vertebrates: digestive system Vitis - chemistry |
| title | Grape Seed Extract Affects Proliferation and Differentiation of Human Intestinal Caco-2 Cells |
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