Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts
The production of cloned animals is a difficult and complex procedure that requires two basic steps. First, the cytoplast must be prepared by the enucleation of metaphase II oocytes. Second, the nucleus is transferred either by fusion or by direct microinjection into the cytoplast. The preparation o...
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| Veröffentlicht in: | Trends in biotechnology (Regular ed.) 2004-06, Vol.22 (6), p.279-283 |
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| creator | Fulka, Josef Loi, Pasqualino Fulka, Helena Ptak, Grazyna Nagai, Taku |
| description | The production of cloned animals is a difficult and complex procedure that requires two basic steps. First, the cytoplast must be prepared by the enucleation of metaphase II oocytes. Second, the nucleus is transferred either by fusion or by direct microinjection into the cytoplast. The preparation of cytoplasts is a crucial step because they must be able to reprogram the transferred nucleus and to secure the development of reconstructed embryos. Moreover, the classical mechanical enucleation of metaphase II oocytes is rather technically difficult, requiring good equipment and considerable micromanipulation skill. For this reason the simplification of this step is permanently in the centre of interest of those scientists who are involved in the production of clones. |
| doi_str_mv | 10.1016/j.tibtech.2004.04.002 |
| format | Article |
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First, the cytoplast must be prepared by the enucleation of metaphase II oocytes. Second, the nucleus is transferred either by fusion or by direct microinjection into the cytoplast. The preparation of cytoplasts is a crucial step because they must be able to reprogram the transferred nucleus and to secure the development of reconstructed embryos. Moreover, the classical mechanical enucleation of metaphase II oocytes is rather technically difficult, requiring good equipment and considerable micromanipulation skill. For this reason the simplification of this step is permanently in the centre of interest of those scientists who are involved in the production of clones.</description><identifier>ISSN: 0167-7799</identifier><identifier>EISSN: 1879-3096</identifier><identifier>DOI: 10.1016/j.tibtech.2004.04.002</identifier><identifier>PMID: 15158056</identifier><identifier>CODEN: TRBIDM</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>animal breeding ; Animals ; Animals, Domestic - embryology ; Animals, Domestic - genetics ; Biological and medical sciences ; Biotechnology ; cell nucleus ; Centrifugation, Density Gradient ; cloning (animals) ; Cloning, Organism - methods ; Cycloheximide - pharmacology ; Cytological Techniques - methods ; cytoplasts ; Demecolcine - pharmacology ; Embryology: invertebrates and vertebrates. Teratology ; enucleation ; Etoposide - pharmacology ; Fundamental and applied biological sciences. Psychology ; Ionomycin - pharmacology ; livestock ; Mammals - embryology ; Mammals - genetics ; Maturation-Promoting Factor - metabolism ; Metaphase - drug effects ; Mice ; Micromanipulation - methods ; Microscopy, Fluorescence ; Microscopy, Polarization ; Molecular embryology ; Nocodazole - pharmacology ; Nuclear Transfer Techniques ; oocytes ; Oocytes - cytology ; Oocytes - drug effects ; Spindle Apparatus - drug effects ; Spindle Apparatus - metabolism ; Sucrose - pharmacology</subject><ispartof>Trends in biotechnology (Regular ed.), 2004-06, Vol.22 (6), p.279-283</ispartof><rights>2004 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-9538c07132a5883b9385f0714ee17da745c02d7c8ce8f11a67e7f84a71e8af493</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0167779904001076$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15805556$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15158056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fulka, Josef</creatorcontrib><creatorcontrib>Loi, Pasqualino</creatorcontrib><creatorcontrib>Fulka, Helena</creatorcontrib><creatorcontrib>Ptak, Grazyna</creatorcontrib><creatorcontrib>Nagai, Taku</creatorcontrib><title>Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts</title><title>Trends in biotechnology (Regular ed.)</title><addtitle>Trends Biotechnol</addtitle><description>The production of cloned animals is a difficult and complex procedure that requires two basic steps. First, the cytoplast must be prepared by the enucleation of metaphase II oocytes. Second, the nucleus is transferred either by fusion or by direct microinjection into the cytoplast. The preparation of cytoplasts is a crucial step because they must be able to reprogram the transferred nucleus and to secure the development of reconstructed embryos. Moreover, the classical mechanical enucleation of metaphase II oocytes is rather technically difficult, requiring good equipment and considerable micromanipulation skill. For this reason the simplification of this step is permanently in the centre of interest of those scientists who are involved in the production of clones.</description><subject>animal breeding</subject><subject>Animals</subject><subject>Animals, Domestic - embryology</subject><subject>Animals, Domestic - genetics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell nucleus</subject><subject>Centrifugation, Density Gradient</subject><subject>cloning (animals)</subject><subject>Cloning, Organism - methods</subject><subject>Cycloheximide - pharmacology</subject><subject>Cytological Techniques - methods</subject><subject>cytoplasts</subject><subject>Demecolcine - pharmacology</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>enucleation</subject><subject>Etoposide - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ionomycin - pharmacology</subject><subject>livestock</subject><subject>Mammals - embryology</subject><subject>Mammals - genetics</subject><subject>Maturation-Promoting Factor - metabolism</subject><subject>Metaphase - drug effects</subject><subject>Mice</subject><subject>Micromanipulation - methods</subject><subject>Microscopy, Fluorescence</subject><subject>Microscopy, Polarization</subject><subject>Molecular embryology</subject><subject>Nocodazole - pharmacology</subject><subject>Nuclear Transfer Techniques</subject><subject>oocytes</subject><subject>Oocytes - cytology</subject><subject>Oocytes - drug effects</subject><subject>Spindle Apparatus - drug effects</subject><subject>Spindle Apparatus - metabolism</subject><subject>Sucrose - pharmacology</subject><issn>0167-7799</issn><issn>1879-3096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c-P1CAUB3BiNO64-ieoXPTWEdoygJeN2fgr2ehB50ze0IfDpC0V6CT730udJnrb5CWE5MPj8YWQl5xtOeO7d6dt9oeM9ritGWu3S7H6EdlwJXXVML17TDbFyUpKra_Is5ROjLFGav6UXHHBhWJityH7b7PtcU40RxiTw0j9SAcYBujTezqG0Y9nSP6MFKYpBrBHTNSFSPMR6RRxggjZh5EGR-19DlMPKafn5IkrDfDFul6T_aePP2-_VHffP3-9_XBXWdHqXGnRKMskb2oQSjUH3Sjhyr5F5LID2QrL6k5aZVE5zmEnUTrVguSowLW6uSZvL33LaL9nTNkMPlnsexgxzMlIrneM1_JByBXjXNeiQHGBNoaUIjozRT9AvDecmSV4czJr8GYJ3izF6nLu1XrBfBiw-3dqTbqANyuAZKF3JW7r03-uKPHXvb44B8HAr1jM_kfNeMPKn-pGL2-5uQgsyZ49RpOsx9Fi5yPabLrgHxj2D-KCrVY</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Fulka, Josef</creator><creator>Loi, Pasqualino</creator><creator>Fulka, Helena</creator><creator>Ptak, Grazyna</creator><creator>Nagai, Taku</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040601</creationdate><title>Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts</title><author>Fulka, Josef ; Loi, Pasqualino ; Fulka, Helena ; Ptak, Grazyna ; Nagai, Taku</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-9538c07132a5883b9385f0714ee17da745c02d7c8ce8f11a67e7f84a71e8af493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>animal breeding</topic><topic>Animals</topic><topic>Animals, Domestic - embryology</topic><topic>Animals, Domestic - genetics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cell nucleus</topic><topic>Centrifugation, Density Gradient</topic><topic>cloning (animals)</topic><topic>Cloning, Organism - methods</topic><topic>Cycloheximide - pharmacology</topic><topic>Cytological Techniques - methods</topic><topic>cytoplasts</topic><topic>Demecolcine - pharmacology</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>enucleation</topic><topic>Etoposide - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ionomycin - pharmacology</topic><topic>livestock</topic><topic>Mammals - embryology</topic><topic>Mammals - genetics</topic><topic>Maturation-Promoting Factor - metabolism</topic><topic>Metaphase - drug effects</topic><topic>Mice</topic><topic>Micromanipulation - methods</topic><topic>Microscopy, Fluorescence</topic><topic>Microscopy, Polarization</topic><topic>Molecular embryology</topic><topic>Nocodazole - pharmacology</topic><topic>Nuclear Transfer Techniques</topic><topic>oocytes</topic><topic>Oocytes - cytology</topic><topic>Oocytes - drug effects</topic><topic>Spindle Apparatus - drug effects</topic><topic>Spindle Apparatus - metabolism</topic><topic>Sucrose - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fulka, Josef</creatorcontrib><creatorcontrib>Loi, Pasqualino</creatorcontrib><creatorcontrib>Fulka, Helena</creatorcontrib><creatorcontrib>Ptak, Grazyna</creatorcontrib><creatorcontrib>Nagai, Taku</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Trends in biotechnology (Regular ed.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fulka, Josef</au><au>Loi, Pasqualino</au><au>Fulka, Helena</au><au>Ptak, Grazyna</au><au>Nagai, Taku</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts</atitle><jtitle>Trends in biotechnology (Regular ed.)</jtitle><addtitle>Trends Biotechnol</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>22</volume><issue>6</issue><spage>279</spage><epage>283</epage><pages>279-283</pages><issn>0167-7799</issn><eissn>1879-3096</eissn><coden>TRBIDM</coden><abstract>The production of cloned animals is a difficult and complex procedure that requires two basic steps. First, the cytoplast must be prepared by the enucleation of metaphase II oocytes. Second, the nucleus is transferred either by fusion or by direct microinjection into the cytoplast. The preparation of cytoplasts is a crucial step because they must be able to reprogram the transferred nucleus and to secure the development of reconstructed embryos. Moreover, the classical mechanical enucleation of metaphase II oocytes is rather technically difficult, requiring good equipment and considerable micromanipulation skill. For this reason the simplification of this step is permanently in the centre of interest of those scientists who are involved in the production of clones.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15158056</pmid><doi>10.1016/j.tibtech.2004.04.002</doi><tpages>5</tpages></addata></record> |
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| subjects | animal breeding Animals Animals, Domestic - embryology Animals, Domestic - genetics Biological and medical sciences Biotechnology cell nucleus Centrifugation, Density Gradient cloning (animals) Cloning, Organism - methods Cycloheximide - pharmacology Cytological Techniques - methods cytoplasts Demecolcine - pharmacology Embryology: invertebrates and vertebrates. Teratology enucleation Etoposide - pharmacology Fundamental and applied biological sciences. Psychology Ionomycin - pharmacology livestock Mammals - embryology Mammals - genetics Maturation-Promoting Factor - metabolism Metaphase - drug effects Mice Micromanipulation - methods Microscopy, Fluorescence Microscopy, Polarization Molecular embryology Nocodazole - pharmacology Nuclear Transfer Techniques oocytes Oocytes - cytology Oocytes - drug effects Spindle Apparatus - drug effects Spindle Apparatus - metabolism Sucrose - pharmacology |
| title | Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts |
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