Molecular Analysis of T-Cell Repertoire in Patients with Graft-Versus-Host Disease after Allogeneic Stem Cell Transplantation
Complementarity-determining region (CDR3) size spectratyping has often been used to analyze the clonal expansion of T-cells. CDR3 size spectratyping has been useful in the analysis of the oligoclonal expansion of T-cells in virus infection, graft-versus-leukemia effect (GVL), graft-versus-host disea...
Gespeichert in:
| Veröffentlicht in: | Leukemia & lymphoma 2004-03, Vol.45 (3), p.481-488 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 488 |
|---|---|
| container_issue | 3 |
| container_start_page | 481 |
| container_title | Leukemia & lymphoma |
| container_volume | 45 |
| creator | Tsutsumi, Yutaka Tanaka, Junji Miura, Yoko Toubai, Tomomi Kato, Naoko Fujisawa, Fumie Toyoshima, Nobuyasu Ota, Shuiti Mori, A Yonezumi, Masakatu Chiba, Koiji Kondo, Takeshi Hasino, Satoshi Kobayasi, Ryouji Masauji, Nobuo Kasai, Masaharu Asaka, Masahiro Imamura, Masahiro |
| description | Complementarity-determining region (CDR3) size spectratyping has often been used to analyze the clonal expansion of T-cells. CDR3 size spectratyping has been useful in the analysis of the oligoclonal expansion of T-cells in virus infection, graft-versus-leukemia effect (GVL), graft-versus-host disease (GVHD), and immune reconstitution of T-cells after allogeneic stem cell transplantation (allo-SCT). We analyzed 26 T cell receptor (TCR)-β-chain subfamilies (VB) in 25 patients who underwent allo-SCT. Fifteen of these patients developed acute GVHD (aGVHD). Many TCR-VB were skewed in the early stage. In these TCR-VB subfamilies, VB6 was most often skewed at the time of skin aGVHD. We then analyzed the average score of the complexity of 26 TCR-VB spectratypings in patients with or without cGVHD. The patients who developed chronic GVHD (cGVHD) had a lower average score of TCR-VB complexity than that of patients without cGVHD (P = 0.010). In particular, the patients who developed the quiescent type and de novo type of cGVHD from 4 months after allo- SCT had a lower average score of TCR-VB complexity at 3 months than that of the patients who had no cGVHD (P = 0.0055). These results suggest that we might be able to consider a possible development of cGVHD by analyzing TCR-VB spectratyping after allo-SCT. |
| doi_str_mv | 10.1080/10428190310001609898 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71958356</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71958356</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-9391997a7c4168de47647db626f19363f6532dde9725fc5e2c49d44e61f9d7d53</originalsourceid><addsrcrecordid>eNqFkV1rFDEUhgdRbK3-A5FceTear8lMbpSyaitUFF29HdLMiZuSSdacDGUv_O9m3QURxF4lhOd9cjhv0zxl9AWjA33JqOQD01QwSilTVA96uNecMsp1yyUV9_d3ydvKyJPmEeJN5Tqt-MPmhHX7ANWnzc8PKYBdgsnkPJqwQ48kObJuVxAC-QxbyCX5DMRH8skUD7EgufVlQy6ycaX9BhkXbC8TFvLGIxgEUt-h6kJI3yGCt-RLgZn8Fq6zibgNJpbqSvFx88CZgPDkeJ41X9-9Xa8u26uPF-9X51etlbIvrRaaad2b3kqmhglkr2Q_XSuuHNNCCac6wacJdM87ZzvgVupJSlDM6amfOnHWPD94tzn9WADLOHu0dSATIS049kx3g-jUnSDTg-x4P1RQHkCbE2IGN26zn03ejYyO-37Gf_VTY8-O_uV6hulP6FhIBV4fAB9dyrO5TTlMYzG7kLKry7MeR3HHF6_-MmzAhLKxJsN4k5ZcS8b_z_gLF7Kx2w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19845278</pqid></control><display><type>article</type><title>Molecular Analysis of T-Cell Repertoire in Patients with Graft-Versus-Host Disease after Allogeneic Stem Cell Transplantation</title><source>Taylor & Francis:Master (3349 titles)</source><source>MEDLINE</source><source>Taylor & Francis Medical Library - CRKN</source><creator>Tsutsumi, Yutaka ; Tanaka, Junji ; Miura, Yoko ; Toubai, Tomomi ; Kato, Naoko ; Fujisawa, Fumie ; Toyoshima, Nobuyasu ; Ota, Shuiti ; Mori, A ; Yonezumi, Masakatu ; Chiba, Koiji ; Kondo, Takeshi ; Hasino, Satoshi ; Kobayasi, Ryouji ; Masauji, Nobuo ; Kasai, Masaharu ; Asaka, Masahiro ; Imamura, Masahiro</creator><creatorcontrib>Tsutsumi, Yutaka ; Tanaka, Junji ; Miura, Yoko ; Toubai, Tomomi ; Kato, Naoko ; Fujisawa, Fumie ; Toyoshima, Nobuyasu ; Ota, Shuiti ; Mori, A ; Yonezumi, Masakatu ; Chiba, Koiji ; Kondo, Takeshi ; Hasino, Satoshi ; Kobayasi, Ryouji ; Masauji, Nobuo ; Kasai, Masaharu ; Asaka, Masahiro ; Imamura, Masahiro</creatorcontrib><description>Complementarity-determining region (CDR3) size spectratyping has often been used to analyze the clonal expansion of T-cells. CDR3 size spectratyping has been useful in the analysis of the oligoclonal expansion of T-cells in virus infection, graft-versus-leukemia effect (GVL), graft-versus-host disease (GVHD), and immune reconstitution of T-cells after allogeneic stem cell transplantation (allo-SCT). We analyzed 26 T cell receptor (TCR)-β-chain subfamilies (VB) in 25 patients who underwent allo-SCT. Fifteen of these patients developed acute GVHD (aGVHD). Many TCR-VB were skewed in the early stage. In these TCR-VB subfamilies, VB6 was most often skewed at the time of skin aGVHD. We then analyzed the average score of the complexity of 26 TCR-VB spectratypings in patients with or without cGVHD. The patients who developed chronic GVHD (cGVHD) had a lower average score of TCR-VB complexity than that of patients without cGVHD (P = 0.010). In particular, the patients who developed the quiescent type and de novo type of cGVHD from 4 months after allo- SCT had a lower average score of TCR-VB complexity at 3 months than that of the patients who had no cGVHD (P = 0.0055). These results suggest that we might be able to consider a possible development of cGVHD by analyzing TCR-VB spectratyping after allo-SCT.</description><identifier>ISSN: 1042-8194</identifier><identifier>EISSN: 1029-2403</identifier><identifier>DOI: 10.1080/10428190310001609898</identifier><identifier>PMID: 15160909</identifier><language>eng</language><publisher>United States: Informa UK Ltd</publisher><subject>Adolescent ; Adult ; allo-SCT ; Blood Cells ; Child ; Chimerism ; Complementarity Determining Regions - analysis ; Female ; Graft vs Host Disease - diagnosis ; Graft vs Host Disease - immunology ; GVHD ; Hematologic Diseases - complications ; Hematologic Diseases - therapy ; Hematopoietic Stem Cell Transplantation - adverse effects ; Humans ; Male ; Middle Aged ; Receptors, Antigen, T-Cell, alpha-beta - analysis ; Spectratyping ; T-Lymphocytes - immunology ; TCR-VB ; Transplantation, Homologous - immunology</subject><ispartof>Leukemia & lymphoma, 2004-03, Vol.45 (3), p.481-488</ispartof><rights>2004 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-9391997a7c4168de47647db626f19363f6532dde9725fc5e2c49d44e61f9d7d53</citedby><cites>FETCH-LOGICAL-c447t-9391997a7c4168de47647db626f19363f6532dde9725fc5e2c49d44e61f9d7d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/10428190310001609898$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/10428190310001609898$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15160909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsutsumi, Yutaka</creatorcontrib><creatorcontrib>Tanaka, Junji</creatorcontrib><creatorcontrib>Miura, Yoko</creatorcontrib><creatorcontrib>Toubai, Tomomi</creatorcontrib><creatorcontrib>Kato, Naoko</creatorcontrib><creatorcontrib>Fujisawa, Fumie</creatorcontrib><creatorcontrib>Toyoshima, Nobuyasu</creatorcontrib><creatorcontrib>Ota, Shuiti</creatorcontrib><creatorcontrib>Mori, A</creatorcontrib><creatorcontrib>Yonezumi, Masakatu</creatorcontrib><creatorcontrib>Chiba, Koiji</creatorcontrib><creatorcontrib>Kondo, Takeshi</creatorcontrib><creatorcontrib>Hasino, Satoshi</creatorcontrib><creatorcontrib>Kobayasi, Ryouji</creatorcontrib><creatorcontrib>Masauji, Nobuo</creatorcontrib><creatorcontrib>Kasai, Masaharu</creatorcontrib><creatorcontrib>Asaka, Masahiro</creatorcontrib><creatorcontrib>Imamura, Masahiro</creatorcontrib><title>Molecular Analysis of T-Cell Repertoire in Patients with Graft-Versus-Host Disease after Allogeneic Stem Cell Transplantation</title><title>Leukemia & lymphoma</title><addtitle>Leuk Lymphoma</addtitle><description>Complementarity-determining region (CDR3) size spectratyping has often been used to analyze the clonal expansion of T-cells. CDR3 size spectratyping has been useful in the analysis of the oligoclonal expansion of T-cells in virus infection, graft-versus-leukemia effect (GVL), graft-versus-host disease (GVHD), and immune reconstitution of T-cells after allogeneic stem cell transplantation (allo-SCT). We analyzed 26 T cell receptor (TCR)-β-chain subfamilies (VB) in 25 patients who underwent allo-SCT. Fifteen of these patients developed acute GVHD (aGVHD). Many TCR-VB were skewed in the early stage. In these TCR-VB subfamilies, VB6 was most often skewed at the time of skin aGVHD. We then analyzed the average score of the complexity of 26 TCR-VB spectratypings in patients with or without cGVHD. The patients who developed chronic GVHD (cGVHD) had a lower average score of TCR-VB complexity than that of patients without cGVHD (P = 0.010). In particular, the patients who developed the quiescent type and de novo type of cGVHD from 4 months after allo- SCT had a lower average score of TCR-VB complexity at 3 months than that of the patients who had no cGVHD (P = 0.0055). These results suggest that we might be able to consider a possible development of cGVHD by analyzing TCR-VB spectratyping after allo-SCT.</description><subject>Adolescent</subject><subject>Adult</subject><subject>allo-SCT</subject><subject>Blood Cells</subject><subject>Child</subject><subject>Chimerism</subject><subject>Complementarity Determining Regions - analysis</subject><subject>Female</subject><subject>Graft vs Host Disease - diagnosis</subject><subject>Graft vs Host Disease - immunology</subject><subject>GVHD</subject><subject>Hematologic Diseases - complications</subject><subject>Hematologic Diseases - therapy</subject><subject>Hematopoietic Stem Cell Transplantation - adverse effects</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - analysis</subject><subject>Spectratyping</subject><subject>T-Lymphocytes - immunology</subject><subject>TCR-VB</subject><subject>Transplantation, Homologous - immunology</subject><issn>1042-8194</issn><issn>1029-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhgdRbK3-A5FceTear8lMbpSyaitUFF29HdLMiZuSSdacDGUv_O9m3QURxF4lhOd9cjhv0zxl9AWjA33JqOQD01QwSilTVA96uNecMsp1yyUV9_d3ydvKyJPmEeJN5Tqt-MPmhHX7ANWnzc8PKYBdgsnkPJqwQ48kObJuVxAC-QxbyCX5DMRH8skUD7EgufVlQy6ycaX9BhkXbC8TFvLGIxgEUt-h6kJI3yGCt-RLgZn8Fq6zibgNJpbqSvFx88CZgPDkeJ41X9-9Xa8u26uPF-9X51etlbIvrRaaad2b3kqmhglkr2Q_XSuuHNNCCac6wacJdM87ZzvgVupJSlDM6amfOnHWPD94tzn9WADLOHu0dSATIS049kx3g-jUnSDTg-x4P1RQHkCbE2IGN26zn03ejYyO-37Gf_VTY8-O_uV6hulP6FhIBV4fAB9dyrO5TTlMYzG7kLKry7MeR3HHF6_-MmzAhLKxJsN4k5ZcS8b_z_gLF7Kx2w</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Tsutsumi, Yutaka</creator><creator>Tanaka, Junji</creator><creator>Miura, Yoko</creator><creator>Toubai, Tomomi</creator><creator>Kato, Naoko</creator><creator>Fujisawa, Fumie</creator><creator>Toyoshima, Nobuyasu</creator><creator>Ota, Shuiti</creator><creator>Mori, A</creator><creator>Yonezumi, Masakatu</creator><creator>Chiba, Koiji</creator><creator>Kondo, Takeshi</creator><creator>Hasino, Satoshi</creator><creator>Kobayasi, Ryouji</creator><creator>Masauji, Nobuo</creator><creator>Kasai, Masaharu</creator><creator>Asaka, Masahiro</creator><creator>Imamura, Masahiro</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040301</creationdate><title>Molecular Analysis of T-Cell Repertoire in Patients with Graft-Versus-Host Disease after Allogeneic Stem Cell Transplantation</title><author>Tsutsumi, Yutaka ; Tanaka, Junji ; Miura, Yoko ; Toubai, Tomomi ; Kato, Naoko ; Fujisawa, Fumie ; Toyoshima, Nobuyasu ; Ota, Shuiti ; Mori, A ; Yonezumi, Masakatu ; Chiba, Koiji ; Kondo, Takeshi ; Hasino, Satoshi ; Kobayasi, Ryouji ; Masauji, Nobuo ; Kasai, Masaharu ; Asaka, Masahiro ; Imamura, Masahiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-9391997a7c4168de47647db626f19363f6532dde9725fc5e2c49d44e61f9d7d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>allo-SCT</topic><topic>Blood Cells</topic><topic>Child</topic><topic>Chimerism</topic><topic>Complementarity Determining Regions - analysis</topic><topic>Female</topic><topic>Graft vs Host Disease - diagnosis</topic><topic>Graft vs Host Disease - immunology</topic><topic>GVHD</topic><topic>Hematologic Diseases - complications</topic><topic>Hematologic Diseases - therapy</topic><topic>Hematopoietic Stem Cell Transplantation - adverse effects</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - analysis</topic><topic>Spectratyping</topic><topic>T-Lymphocytes - immunology</topic><topic>TCR-VB</topic><topic>Transplantation, Homologous - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsutsumi, Yutaka</creatorcontrib><creatorcontrib>Tanaka, Junji</creatorcontrib><creatorcontrib>Miura, Yoko</creatorcontrib><creatorcontrib>Toubai, Tomomi</creatorcontrib><creatorcontrib>Kato, Naoko</creatorcontrib><creatorcontrib>Fujisawa, Fumie</creatorcontrib><creatorcontrib>Toyoshima, Nobuyasu</creatorcontrib><creatorcontrib>Ota, Shuiti</creatorcontrib><creatorcontrib>Mori, A</creatorcontrib><creatorcontrib>Yonezumi, Masakatu</creatorcontrib><creatorcontrib>Chiba, Koiji</creatorcontrib><creatorcontrib>Kondo, Takeshi</creatorcontrib><creatorcontrib>Hasino, Satoshi</creatorcontrib><creatorcontrib>Kobayasi, Ryouji</creatorcontrib><creatorcontrib>Masauji, Nobuo</creatorcontrib><creatorcontrib>Kasai, Masaharu</creatorcontrib><creatorcontrib>Asaka, Masahiro</creatorcontrib><creatorcontrib>Imamura, Masahiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia & lymphoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsutsumi, Yutaka</au><au>Tanaka, Junji</au><au>Miura, Yoko</au><au>Toubai, Tomomi</au><au>Kato, Naoko</au><au>Fujisawa, Fumie</au><au>Toyoshima, Nobuyasu</au><au>Ota, Shuiti</au><au>Mori, A</au><au>Yonezumi, Masakatu</au><au>Chiba, Koiji</au><au>Kondo, Takeshi</au><au>Hasino, Satoshi</au><au>Kobayasi, Ryouji</au><au>Masauji, Nobuo</au><au>Kasai, Masaharu</au><au>Asaka, Masahiro</au><au>Imamura, Masahiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Analysis of T-Cell Repertoire in Patients with Graft-Versus-Host Disease after Allogeneic Stem Cell Transplantation</atitle><jtitle>Leukemia & lymphoma</jtitle><addtitle>Leuk Lymphoma</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>45</volume><issue>3</issue><spage>481</spage><epage>488</epage><pages>481-488</pages><issn>1042-8194</issn><eissn>1029-2403</eissn><abstract>Complementarity-determining region (CDR3) size spectratyping has often been used to analyze the clonal expansion of T-cells. CDR3 size spectratyping has been useful in the analysis of the oligoclonal expansion of T-cells in virus infection, graft-versus-leukemia effect (GVL), graft-versus-host disease (GVHD), and immune reconstitution of T-cells after allogeneic stem cell transplantation (allo-SCT). We analyzed 26 T cell receptor (TCR)-β-chain subfamilies (VB) in 25 patients who underwent allo-SCT. Fifteen of these patients developed acute GVHD (aGVHD). Many TCR-VB were skewed in the early stage. In these TCR-VB subfamilies, VB6 was most often skewed at the time of skin aGVHD. We then analyzed the average score of the complexity of 26 TCR-VB spectratypings in patients with or without cGVHD. The patients who developed chronic GVHD (cGVHD) had a lower average score of TCR-VB complexity than that of patients without cGVHD (P = 0.010). In particular, the patients who developed the quiescent type and de novo type of cGVHD from 4 months after allo- SCT had a lower average score of TCR-VB complexity at 3 months than that of the patients who had no cGVHD (P = 0.0055). These results suggest that we might be able to consider a possible development of cGVHD by analyzing TCR-VB spectratyping after allo-SCT.</abstract><cop>United States</cop><pub>Informa UK Ltd</pub><pmid>15160909</pmid><doi>10.1080/10428190310001609898</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1042-8194 |
| ispartof | Leukemia & lymphoma, 2004-03, Vol.45 (3), p.481-488 |
| issn | 1042-8194 1029-2403 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71958356 |
| source | Taylor & Francis:Master (3349 titles); MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | Adolescent Adult allo-SCT Blood Cells Child Chimerism Complementarity Determining Regions - analysis Female Graft vs Host Disease - diagnosis Graft vs Host Disease - immunology GVHD Hematologic Diseases - complications Hematologic Diseases - therapy Hematopoietic Stem Cell Transplantation - adverse effects Humans Male Middle Aged Receptors, Antigen, T-Cell, alpha-beta - analysis Spectratyping T-Lymphocytes - immunology TCR-VB Transplantation, Homologous - immunology |
| title | Molecular Analysis of T-Cell Repertoire in Patients with Graft-Versus-Host Disease after Allogeneic Stem Cell Transplantation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T04%3A16%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Analysis%20of%20T-Cell%20Repertoire%20in%20Patients%20with%20Graft-Versus-Host%20Disease%20after%20Allogeneic%20Stem%20Cell%20Transplantation&rft.jtitle=Leukemia%20&%20lymphoma&rft.au=Tsutsumi,%20Yutaka&rft.date=2004-03-01&rft.volume=45&rft.issue=3&rft.spage=481&rft.epage=488&rft.pages=481-488&rft.issn=1042-8194&rft.eissn=1029-2403&rft_id=info:doi/10.1080/10428190310001609898&rft_dat=%3Cproquest_cross%3E71958356%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19845278&rft_id=info:pmid/15160909&rfr_iscdi=true |