Glucose, lactate, and pyruvate response in an experimental model of microvascular flap ischemia and reperfusion: A microdialysis study
Early diagnosis of postoperative perfusion failure is essential in microsurgical tissue transfer. In order to determine if microdialysis could be used in diagnosing flap ischemia, we tested this method in an experimental pig model. Sixty‐six flaps (34 myocutaneous and 29 cutaneous) were created in 1...
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| Veröffentlicht in: | Microsurgery 2004, Vol.24 (3), p.223-231 |
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| creator | Setälä, Leena P. Korvenoja, E. Maj-lén Härmä, Markku A. Alhava, Esko M. Uusaro, Ari V. Tenhunen, Jyrki J. |
| description | Early diagnosis of postoperative perfusion failure is essential in microsurgical tissue transfer. In order to determine if microdialysis could be used in diagnosing flap ischemia, we tested this method in an experimental pig model. Sixty‐six flaps (34 myocutaneous and 29 cutaneous) were created in 18 anesthetized pigs. During the experiment, secondary ischemia was induced for 5 h by selective clamping of the artery (20 flaps) or vein (21 flaps). Glucose, lactate, and pyruvate concentrations were measured hourly from the muscular and dermal layers. We found that decreasing glucose levels and increasing lactate concentrations were associated with arterial and venous occlusions from the first hour of ischemia. In venous ischemia, lactate concentrations remained lower than those in arterial ischemia. The increase in lactate‐to‐pyruvate and lactate‐to‐glucose ratios was related to ischemia and also discriminated arterial occlusion from venous occlusion. In conclusion, microdialysis can be used to facilitate early detection of ischemia. © 2004 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/micr.20045 |
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Maj-lén ; Härmä, Markku A. ; Alhava, Esko M. ; Uusaro, Ari V. ; Tenhunen, Jyrki J.</creator><creatorcontrib>Setälä, Leena P. ; Korvenoja, E. Maj-lén ; Härmä, Markku A. ; Alhava, Esko M. ; Uusaro, Ari V. ; Tenhunen, Jyrki J.</creatorcontrib><description>Early diagnosis of postoperative perfusion failure is essential in microsurgical tissue transfer. In order to determine if microdialysis could be used in diagnosing flap ischemia, we tested this method in an experimental pig model. Sixty‐six flaps (34 myocutaneous and 29 cutaneous) were created in 18 anesthetized pigs. During the experiment, secondary ischemia was induced for 5 h by selective clamping of the artery (20 flaps) or vein (21 flaps). Glucose, lactate, and pyruvate concentrations were measured hourly from the muscular and dermal layers. We found that decreasing glucose levels and increasing lactate concentrations were associated with arterial and venous occlusions from the first hour of ischemia. In venous ischemia, lactate concentrations remained lower than those in arterial ischemia. The increase in lactate‐to‐pyruvate and lactate‐to‐glucose ratios was related to ischemia and also discriminated arterial occlusion from venous occlusion. In conclusion, microdialysis can be used to facilitate early detection of ischemia. © 2004 Wiley‐Liss, Inc.</description><identifier>ISSN: 0738-1085</identifier><identifier>EISSN: 1098-2752</identifier><identifier>DOI: 10.1002/micr.20045</identifier><identifier>PMID: 15160382</identifier><identifier>CODEN: MSRGDQ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Biomarkers ; Combined surgery. Multiple transplantations ; Disease Models, Animal ; Female ; Glucose - metabolism ; Graft Rejection ; Graft Survival ; Ischemia - diagnosis ; Lactates - metabolism ; Medical sciences ; Microdialysis - methods ; Probability ; Pyruvates - metabolism ; Rectus Abdominis - blood supply ; Rectus Abdominis - transplantation ; Reperfusion Injury - diagnosis ; ROC Curve ; Sensitivity and Specificity ; Statistics, Nonparametric ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical Flaps - adverse effects ; Surgical Flaps - blood supply ; Sus scrofa</subject><ispartof>Microsurgery, 2004, Vol.24 (3), p.223-231</ispartof><rights>Copyright © 2004 Wiley‐Liss, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4595-ef9ed1b7e0fc4b37cc3db79cf0066ca5229fee69672c1d2bf8f3dc85507fb71e3</citedby><cites>FETCH-LOGICAL-c4595-ef9ed1b7e0fc4b37cc3db79cf0066ca5229fee69672c1d2bf8f3dc85507fb71e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmicr.20045$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmicr.20045$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15796581$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15160382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Setälä, Leena P.</creatorcontrib><creatorcontrib>Korvenoja, E. Maj-lén</creatorcontrib><creatorcontrib>Härmä, Markku A.</creatorcontrib><creatorcontrib>Alhava, Esko M.</creatorcontrib><creatorcontrib>Uusaro, Ari V.</creatorcontrib><creatorcontrib>Tenhunen, Jyrki J.</creatorcontrib><title>Glucose, lactate, and pyruvate response in an experimental model of microvascular flap ischemia and reperfusion: A microdialysis study</title><title>Microsurgery</title><addtitle>Microsurgery</addtitle><description>Early diagnosis of postoperative perfusion failure is essential in microsurgical tissue transfer. In order to determine if microdialysis could be used in diagnosing flap ischemia, we tested this method in an experimental pig model. Sixty‐six flaps (34 myocutaneous and 29 cutaneous) were created in 18 anesthetized pigs. During the experiment, secondary ischemia was induced for 5 h by selective clamping of the artery (20 flaps) or vein (21 flaps). Glucose, lactate, and pyruvate concentrations were measured hourly from the muscular and dermal layers. We found that decreasing glucose levels and increasing lactate concentrations were associated with arterial and venous occlusions from the first hour of ischemia. In venous ischemia, lactate concentrations remained lower than those in arterial ischemia. The increase in lactate‐to‐pyruvate and lactate‐to‐glucose ratios was related to ischemia and also discriminated arterial occlusion from venous occlusion. In conclusion, microdialysis can be used to facilitate early detection of ischemia. © 2004 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Combined surgery. Multiple transplantations</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Glucose - metabolism</subject><subject>Graft Rejection</subject><subject>Graft Survival</subject><subject>Ischemia - diagnosis</subject><subject>Lactates - metabolism</subject><subject>Medical sciences</subject><subject>Microdialysis - methods</subject><subject>Probability</subject><subject>Pyruvates - metabolism</subject><subject>Rectus Abdominis - blood supply</subject><subject>Rectus Abdominis - transplantation</subject><subject>Reperfusion Injury - diagnosis</subject><subject>ROC Curve</subject><subject>Sensitivity and Specificity</subject><subject>Statistics, Nonparametric</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical Flaps - adverse effects</subject><subject>Surgical Flaps - blood supply</subject><subject>Sus scrofa</subject><issn>0738-1085</issn><issn>1098-2752</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1DAUhS0EokNhww9A3sACkeJHHMfdVSM6VCoFIRASG8txroXBeWAnpfkD_G48zfBYsbItf-fccw9Cjyk5oYSwl5238YQRUoo7aEOJqgsmBbuLNkTyuqCkFkfoQUpfCSFKSXUfHVFBK8JrtkE_d2G2Q4IXOBg7mSlfTN_icYnzdX7hCGkc-gTY9_kDw80I0XfQTybgbmgh4MHhfYDh2iQ7BxOxC2bEPtkv0Hlz6xYhq9yc_NCf4rMVb70JS_IJp2lul4fonjMhwaPDeYw-nr_6sH1dXL7dXWzPLgtbCiUKcApa2kggzpYNl9bytpHKOkKqyhrBmHIAlaoks7Rljasdb20tBJGukRT4MXq2-o5x-D5DmnSXk0IIpodhTlpSJcqsz-DzFcxRU4rg9Jj3NnHRlOh963q_hb5tPcNPDq5z00H7Fz3UnIGnByCXZIKLprc-_cNJVYmaZo6u3A8fYPnPSP3mYvv-9_Bi1fg0wc0fjYnfdCW5FPrT1U7Td7tz_vmq1CX_BepHrHU</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Setälä, Leena P.</creator><creator>Korvenoja, E. Maj-lén</creator><creator>Härmä, Markku A.</creator><creator>Alhava, Esko M.</creator><creator>Uusaro, Ari V.</creator><creator>Tenhunen, Jyrki J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Glucose, lactate, and pyruvate response in an experimental model of microvascular flap ischemia and reperfusion: A microdialysis study</title><author>Setälä, Leena P. ; Korvenoja, E. Maj-lén ; Härmä, Markku A. ; Alhava, Esko M. ; Uusaro, Ari V. ; Tenhunen, Jyrki J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4595-ef9ed1b7e0fc4b37cc3db79cf0066ca5229fee69672c1d2bf8f3dc85507fb71e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Combined surgery. Multiple transplantations</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Glucose - metabolism</topic><topic>Graft Rejection</topic><topic>Graft Survival</topic><topic>Ischemia - diagnosis</topic><topic>Lactates - metabolism</topic><topic>Medical sciences</topic><topic>Microdialysis - methods</topic><topic>Probability</topic><topic>Pyruvates - metabolism</topic><topic>Rectus Abdominis - blood supply</topic><topic>Rectus Abdominis - transplantation</topic><topic>Reperfusion Injury - diagnosis</topic><topic>ROC Curve</topic><topic>Sensitivity and Specificity</topic><topic>Statistics, Nonparametric</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical Flaps - adverse effects</topic><topic>Surgical Flaps - blood supply</topic><topic>Sus scrofa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Setälä, Leena P.</creatorcontrib><creatorcontrib>Korvenoja, E. Maj-lén</creatorcontrib><creatorcontrib>Härmä, Markku A.</creatorcontrib><creatorcontrib>Alhava, Esko M.</creatorcontrib><creatorcontrib>Uusaro, Ari V.</creatorcontrib><creatorcontrib>Tenhunen, Jyrki J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microsurgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Setälä, Leena P.</au><au>Korvenoja, E. 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During the experiment, secondary ischemia was induced for 5 h by selective clamping of the artery (20 flaps) or vein (21 flaps). Glucose, lactate, and pyruvate concentrations were measured hourly from the muscular and dermal layers. We found that decreasing glucose levels and increasing lactate concentrations were associated with arterial and venous occlusions from the first hour of ischemia. In venous ischemia, lactate concentrations remained lower than those in arterial ischemia. The increase in lactate‐to‐pyruvate and lactate‐to‐glucose ratios was related to ischemia and also discriminated arterial occlusion from venous occlusion. In conclusion, microdialysis can be used to facilitate early detection of ischemia. © 2004 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15160382</pmid><doi>10.1002/micr.20045</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Biomarkers Combined surgery. Multiple transplantations Disease Models, Animal Female Glucose - metabolism Graft Rejection Graft Survival Ischemia - diagnosis Lactates - metabolism Medical sciences Microdialysis - methods Probability Pyruvates - metabolism Rectus Abdominis - blood supply Rectus Abdominis - transplantation Reperfusion Injury - diagnosis ROC Curve Sensitivity and Specificity Statistics, Nonparametric Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgical Flaps - adverse effects Surgical Flaps - blood supply Sus scrofa |
| title | Glucose, lactate, and pyruvate response in an experimental model of microvascular flap ischemia and reperfusion: A microdialysis study |
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