Pre-Steady-State Kinetic Studies of the Fidelity and Mechanism of Polymerization Catalyzed by Truncated Human DNA Polymerase λ

DNA polymerase λ (Polλ), a member of the X-family DNA polymerases, possesses an N-terminal BRCT domain, a proline-rich domain, and a C-terminal polymerase β-like domain (tPolλ). In this paper, we determined a minimal kinetic mechanism and the fidelity of tPolλ using pre-steady-state kinetic analysis...

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