Rapid Identification of Mitochondrial Cytochrome B Haplotypes by Single Strand Conformation Polymorphism in Lutzomyia longipalpis (Diptera: Psychodidae) Populations
Newly designed primers amplified a 365-bp fragment including the 3′ end of mitochondrial cytochrome b (cyt b). Field collected specimens of the sand fly, Lutzomyia longipalpis (Lutz and Neiva), from two Brazil locations provided test DNA. The primers, based on consensus sequences from a taxonomic sp...
Gespeichert in:
| Veröffentlicht in: | Journal of medical entomology 2002-07, Vol.39 (4), p.689-694 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 694 |
|---|---|
| container_issue | 4 |
| container_start_page | 689 |
| container_title | Journal of medical entomology |
| container_volume | 39 |
| creator | Hodgkinson, Virginia H. Birungi, Josephine Haghpanah, Michelle Joshi, Swati Munstermann, Leonard E. |
| description | Newly designed primers amplified a 365-bp fragment including the 3′ end of mitochondrial cytochrome b (cyt b). Field collected specimens of the sand fly, Lutzomyia longipalpis (Lutz and Neiva), from two Brazil locations provided test DNA. The primers, based on consensus sequences from a taxonomic spectrum of arthropods, have also amplified DNA successfully from Lutzomyia shannoni Dyar, Lutzomyia cruzi (Mangabeira), and the mosquito Aedes albopictus (Skuse). Single strand conformation polymorphism (SSCP) was used to recognize the presence of L. longipalpis haplotypes; these were verified subsequently by sequencing the polymerase chain reaction products. The sensitivity of SSCP was demonstrated by (1) the detection of single nucleotide differences in SSCP variants and (2) no sequence variation in specimens with the same SSCP mobility. The application of new primers and the SSCP technique provided a valuable addition to available population genetic tools: they increased the efficiency of detection of variability in the cyt b gene and decreased the time required for screening large numbers of specimens to detect nucleotide variation. |
| doi_str_mv | 10.1603/0022-2585-39.4.689 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_71950183</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A535150604</galeid><sourcerecordid>A535150604</sourcerecordid><originalsourceid>FETCH-LOGICAL-b519t-f4cb610086770b8a69fb10ba5cd0b53c70ab9f8a1db70ad00a489636837741953</originalsourceid><addsrcrecordid>eNqNkctu1DAUhiMEokPhBVggb0CwyGDHudjdleHSSoOoKKwtX6cHOXGwk0V4Hh4UhxnRLfLCPtb3_-cc_UXxnOAtaTF9i3FVlVXDmpLybb1tGX9QbAinrKx4xR4Wm3_AWfEkpR8YY0Zq_rg4IxWpa4rbTfH7qxzBoGtjhwkcaDlBGFBw6DNMQd-FwUSQHu2WtYqht-gdupKjD9My2oTUgm5hOHiLbqcoB4N2YXAh9kebm-CXPsTxDlKPYED7efoV-gUk8mE4wCj9CAm9fg_jZKO8QDdpyS0NGGnfZPE4-78-6WnxyEmf7LPTfV58__jh2-6q3H_5dL273JeqIXwqXa1VS_KSbddhxWTLnSJYyUYbrBqqOywVd0wSo_LTYCxrxlvaMtp1NeENPS9eHX3HGH7ONk2ih6St93KwYU6iyxAmjGZwewQP0lsBeeW8vc7H2B50GKyD_H_Z0IY0uMV1FlRHgY4hpWidGCP0Mi6CYLGGKdasxJqVoFzUIoeZRS9O48yqt-ZeckovAy9PgExaepcT0JDuOcoqhuu1Oz5yCkIe7n96_wFt8riN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71950183</pqid></control><display><type>article</type><title>Rapid Identification of Mitochondrial Cytochrome B Haplotypes by Single Strand Conformation Polymorphism in Lutzomyia longipalpis (Diptera: Psychodidae) Populations</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>BioOne Complete</source><creator>Hodgkinson, Virginia H. ; Birungi, Josephine ; Haghpanah, Michelle ; Joshi, Swati ; Munstermann, Leonard E.</creator><creatorcontrib>Hodgkinson, Virginia H. ; Birungi, Josephine ; Haghpanah, Michelle ; Joshi, Swati ; Munstermann, Leonard E.</creatorcontrib><description>Newly designed primers amplified a 365-bp fragment including the 3′ end of mitochondrial cytochrome b (cyt b). Field collected specimens of the sand fly, Lutzomyia longipalpis (Lutz and Neiva), from two Brazil locations provided test DNA. The primers, based on consensus sequences from a taxonomic spectrum of arthropods, have also amplified DNA successfully from Lutzomyia shannoni Dyar, Lutzomyia cruzi (Mangabeira), and the mosquito Aedes albopictus (Skuse). Single strand conformation polymorphism (SSCP) was used to recognize the presence of L. longipalpis haplotypes; these were verified subsequently by sequencing the polymerase chain reaction products. The sensitivity of SSCP was demonstrated by (1) the detection of single nucleotide differences in SSCP variants and (2) no sequence variation in specimens with the same SSCP mobility. The application of new primers and the SSCP technique provided a valuable addition to available population genetic tools: they increased the efficiency of detection of variability in the cyt b gene and decreased the time required for screening large numbers of specimens to detect nucleotide variation.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>EISSN: 0022-2585</identifier><identifier>DOI: 10.1603/0022-2585-39.4.689</identifier><identifier>PMID: 12144306</identifier><identifier>CODEN: JMENA6</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Animals ; Asian tiger mosquito ; Biological and medical sciences ; Cytochrome b ; Cytochrome b Group - classification ; Cytochrome b Group - genetics ; DNA, Mitochondrial ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic aspects ; Genetics of eukaryotes. Biological and molecular evolution ; Haplotypes ; Invertebrata ; Lutzomyia longipalpis ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Mitochondria - metabolism ; molecular methods ; Polymorphism, Single-Stranded Conformational ; Population genetics ; Population genetics, reproduction patterns ; Psychodidae - genetics ; sand fly ; Sensitivity and Specificity ; SHORT COMMUNICATION ; single strand conformation polymorphism ; Time Factors ; Vectors. Intermediate hosts</subject><ispartof>Journal of medical entomology, 2002-07, Vol.39 (4), p.689-694</ispartof><rights>Entomological Society of America</rights><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2002 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b519t-f4cb610086770b8a69fb10ba5cd0b53c70ab9f8a1db70ad00a489636837741953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1603/0022-2585-39.4.689$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,776,780,26955,27901,27902,52338</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13828044$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12144306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hodgkinson, Virginia H.</creatorcontrib><creatorcontrib>Birungi, Josephine</creatorcontrib><creatorcontrib>Haghpanah, Michelle</creatorcontrib><creatorcontrib>Joshi, Swati</creatorcontrib><creatorcontrib>Munstermann, Leonard E.</creatorcontrib><title>Rapid Identification of Mitochondrial Cytochrome B Haplotypes by Single Strand Conformation Polymorphism in Lutzomyia longipalpis (Diptera: Psychodidae) Populations</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>Newly designed primers amplified a 365-bp fragment including the 3′ end of mitochondrial cytochrome b (cyt b). Field collected specimens of the sand fly, Lutzomyia longipalpis (Lutz and Neiva), from two Brazil locations provided test DNA. The primers, based on consensus sequences from a taxonomic spectrum of arthropods, have also amplified DNA successfully from Lutzomyia shannoni Dyar, Lutzomyia cruzi (Mangabeira), and the mosquito Aedes albopictus (Skuse). Single strand conformation polymorphism (SSCP) was used to recognize the presence of L. longipalpis haplotypes; these were verified subsequently by sequencing the polymerase chain reaction products. The sensitivity of SSCP was demonstrated by (1) the detection of single nucleotide differences in SSCP variants and (2) no sequence variation in specimens with the same SSCP mobility. The application of new primers and the SSCP technique provided a valuable addition to available population genetic tools: they increased the efficiency of detection of variability in the cyt b gene and decreased the time required for screening large numbers of specimens to detect nucleotide variation.</description><subject>Animals</subject><subject>Asian tiger mosquito</subject><subject>Biological and medical sciences</subject><subject>Cytochrome b</subject><subject>Cytochrome b Group - classification</subject><subject>Cytochrome b Group - genetics</subject><subject>DNA, Mitochondrial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Haplotypes</subject><subject>Invertebrata</subject><subject>Lutzomyia longipalpis</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Mitochondria - metabolism</subject><subject>molecular methods</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Population genetics</subject><subject>Population genetics, reproduction patterns</subject><subject>Psychodidae - genetics</subject><subject>sand fly</subject><subject>Sensitivity and Specificity</subject><subject>SHORT COMMUNICATION</subject><subject>single strand conformation polymorphism</subject><subject>Time Factors</subject><subject>Vectors. Intermediate hosts</subject><issn>0022-2585</issn><issn>1938-2928</issn><issn>0022-2585</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhiMEokPhBVggb0CwyGDHudjdleHSSoOoKKwtX6cHOXGwk0V4Hh4UhxnRLfLCPtb3_-cc_UXxnOAtaTF9i3FVlVXDmpLybb1tGX9QbAinrKx4xR4Wm3_AWfEkpR8YY0Zq_rg4IxWpa4rbTfH7qxzBoGtjhwkcaDlBGFBw6DNMQd-FwUSQHu2WtYqht-gdupKjD9My2oTUgm5hOHiLbqcoB4N2YXAh9kebm-CXPsTxDlKPYED7efoV-gUk8mE4wCj9CAm9fg_jZKO8QDdpyS0NGGnfZPE4-78-6WnxyEmf7LPTfV58__jh2-6q3H_5dL273JeqIXwqXa1VS_KSbddhxWTLnSJYyUYbrBqqOywVd0wSo_LTYCxrxlvaMtp1NeENPS9eHX3HGH7ONk2ih6St93KwYU6iyxAmjGZwewQP0lsBeeW8vc7H2B50GKyD_H_Z0IY0uMV1FlRHgY4hpWidGCP0Mi6CYLGGKdasxJqVoFzUIoeZRS9O48yqt-ZeckovAy9PgExaepcT0JDuOcoqhuu1Oz5yCkIe7n96_wFt8riN</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Hodgkinson, Virginia H.</creator><creator>Birungi, Josephine</creator><creator>Haghpanah, Michelle</creator><creator>Joshi, Swati</creator><creator>Munstermann, Leonard E.</creator><general>Entomological Society of America</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Rapid Identification of Mitochondrial Cytochrome B Haplotypes by Single Strand Conformation Polymorphism in Lutzomyia longipalpis (Diptera: Psychodidae) Populations</title><author>Hodgkinson, Virginia H. ; Birungi, Josephine ; Haghpanah, Michelle ; Joshi, Swati ; Munstermann, Leonard E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b519t-f4cb610086770b8a69fb10ba5cd0b53c70ab9f8a1db70ad00a489636837741953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Asian tiger mosquito</topic><topic>Biological and medical sciences</topic><topic>Cytochrome b</topic><topic>Cytochrome b Group - classification</topic><topic>Cytochrome b Group - genetics</topic><topic>DNA, Mitochondrial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Haplotypes</topic><topic>Invertebrata</topic><topic>Lutzomyia longipalpis</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>Mitochondria - metabolism</topic><topic>molecular methods</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Population genetics</topic><topic>Population genetics, reproduction patterns</topic><topic>Psychodidae - genetics</topic><topic>sand fly</topic><topic>Sensitivity and Specificity</topic><topic>SHORT COMMUNICATION</topic><topic>single strand conformation polymorphism</topic><topic>Time Factors</topic><topic>Vectors. Intermediate hosts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hodgkinson, Virginia H.</creatorcontrib><creatorcontrib>Birungi, Josephine</creatorcontrib><creatorcontrib>Haghpanah, Michelle</creatorcontrib><creatorcontrib>Joshi, Swati</creatorcontrib><creatorcontrib>Munstermann, Leonard E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hodgkinson, Virginia H.</au><au>Birungi, Josephine</au><au>Haghpanah, Michelle</au><au>Joshi, Swati</au><au>Munstermann, Leonard E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Identification of Mitochondrial Cytochrome B Haplotypes by Single Strand Conformation Polymorphism in Lutzomyia longipalpis (Diptera: Psychodidae) Populations</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>39</volume><issue>4</issue><spage>689</spage><epage>694</epage><pages>689-694</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><eissn>0022-2585</eissn><coden>JMENA6</coden><abstract>Newly designed primers amplified a 365-bp fragment including the 3′ end of mitochondrial cytochrome b (cyt b). Field collected specimens of the sand fly, Lutzomyia longipalpis (Lutz and Neiva), from two Brazil locations provided test DNA. The primers, based on consensus sequences from a taxonomic spectrum of arthropods, have also amplified DNA successfully from Lutzomyia shannoni Dyar, Lutzomyia cruzi (Mangabeira), and the mosquito Aedes albopictus (Skuse). Single strand conformation polymorphism (SSCP) was used to recognize the presence of L. longipalpis haplotypes; these were verified subsequently by sequencing the polymerase chain reaction products. The sensitivity of SSCP was demonstrated by (1) the detection of single nucleotide differences in SSCP variants and (2) no sequence variation in specimens with the same SSCP mobility. The application of new primers and the SSCP technique provided a valuable addition to available population genetic tools: they increased the efficiency of detection of variability in the cyt b gene and decreased the time required for screening large numbers of specimens to detect nucleotide variation.</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>12144306</pmid><doi>10.1603/0022-2585-39.4.689</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 2002-07, Vol.39 (4), p.689-694 |
| issn | 0022-2585 1938-2928 0022-2585 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71950183 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; BioOne Complete |
| subjects | Animals Asian tiger mosquito Biological and medical sciences Cytochrome b Cytochrome b Group - classification Cytochrome b Group - genetics DNA, Mitochondrial Fundamental and applied biological sciences. Psychology Genes Genetic aspects Genetics of eukaryotes. Biological and molecular evolution Haplotypes Invertebrata Lutzomyia longipalpis Medically important nuisances and vectors, pests of stored products and materials: population survey and control Mitochondria - metabolism molecular methods Polymorphism, Single-Stranded Conformational Population genetics Population genetics, reproduction patterns Psychodidae - genetics sand fly Sensitivity and Specificity SHORT COMMUNICATION single strand conformation polymorphism Time Factors Vectors. Intermediate hosts |
| title | Rapid Identification of Mitochondrial Cytochrome B Haplotypes by Single Strand Conformation Polymorphism in Lutzomyia longipalpis (Diptera: Psychodidae) Populations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T13%3A25%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20Identification%20of%20Mitochondrial%20Cytochrome%20B%20Haplotypes%20by%20Single%20Strand%20Conformation%20Polymorphism%20in%20Lutzomyia%20longipalpis%20(Diptera:%20Psychodidae)%20Populations&rft.jtitle=Journal%20of%20medical%20entomology&rft.au=Hodgkinson,%20Virginia%20H.&rft.date=2002-07-01&rft.volume=39&rft.issue=4&rft.spage=689&rft.epage=694&rft.pages=689-694&rft.issn=0022-2585&rft.eissn=1938-2928&rft.coden=JMENA6&rft_id=info:doi/10.1603/0022-2585-39.4.689&rft_dat=%3Cgale_proqu%3EA535150604%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71950183&rft_id=info:pmid/12144306&rft_galeid=A535150604&rfr_iscdi=true |