Functional Analysis of the Matricellular Protein SPARC with Novel Monoclonal Antibodies
SPARC (osteonectin, BM-40) is a matricellular glycoprotein that is expressed in many embryogenic and adult tissues undergoing remodeling or repair. SPARC modulates cellular interaction with the extracellular matrix (ECM), inhibits cell adhesion and proliferation, and regulates growth factor activity...
Gespeichert in:
| Veröffentlicht in: | The journal of histochemistry and cytochemistry 2004-06, Vol.52 (6), p.723-733 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 733 |
|---|---|
| container_issue | 6 |
| container_start_page | 723 |
| container_title | The journal of histochemistry and cytochemistry |
| container_volume | 52 |
| creator | Sweetwyne, Mariya T Brekken, Rolf A Workman, Gail Bradshaw, Amy D Carbon, Juliet Siadak, Anthony W Murri, Carrie Sage, E. Helene |
| description | SPARC (osteonectin, BM-40) is a matricellular glycoprotein that is expressed in many embryogenic and adult tissues undergoing remodeling or repair. SPARC modulates cellular interaction with the extracellular matrix (ECM), inhibits cell adhesion and proliferation, and regulates growth factor activity. To explore further the function and activity of this protein in tissue homeostasis, we have developed several monoclonal antibodies (MAbs) that recognize distinct epitopes on SPARC. The MAbs bind to SPARC with high affinity and identify SPARC by ELISA, Western blotting, immunoprecipitation, immunocytochemistry, and/or immunohistochemistry. The MAbs were also characterized in functional assays for potential alteration of SPARC activity. SPARC binds to collagen I and laminin-1 through an epitope defined by MAb 293; this epitope is not involved in the binding of SPARC to collagen III. The other MAbs did not interfere with the binding of SPARC to collagen I or III or laminin-1. Inhibition of the anti-adhesive effect of SPARC on endothelial cells by MAb 236 was also observed. Functional analysis of SPARC in the presence of these novel MAbs now confirms that the activities ascribed to this matricellular protein can be assigned to discrete subdomains. |
| doi_str_mv | 10.1369/jhc.3A6153.2004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71950123</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1369_jhc.3A6153.2004</sage_id><sourcerecordid>71950123</sourcerecordid><originalsourceid>FETCH-LOGICAL-c401t-1c6ace565c13a0bef85b09ad4def1ed861381c7942104e06a92293f36ef8fdef3</originalsourceid><addsrcrecordid>eNp9kE1r3DAQQEVISbZJz70VXdJTvdHow2sflyVpC0kbmpYehVYex1q0ViLZNfn31eKF3noZwfD0GB4h74EtQZT19a6zS7EuQYklZ0yekAUoBYViUp6SBWOcF3khz8nblHaMgZSqOiPnoEAxXsGC_L4dezu40BtP13m8JpdoaOnQIb03Q3QWvR-9ifQhhgFdTx8f1j82dHJDR7-FP-jpfeiD9UfD4LahcZguyZvW-ITvju8F-XV783Pzpbj7_vnrZn1XWMlgKMCWxqIqlQVh2BbbSm1ZbRrZYAvYVCWICuyqlhyYRFaamvNatKLMZJsZcUE-zt7nGF5GTIPeu3S42fQYxqRXUCsGXGTwegZtDClFbPVzdHsTXzUwfWipc0s9t9SHlvnHh6N63O6x-ccf42Xg0wwk84R6F8aYG6T_-K5mvHNP3eQi6rQ33mc76GmaFNelXuVL_wKrp4r9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71950123</pqid></control><display><type>article</type><title>Functional Analysis of the Matricellular Protein SPARC with Novel Monoclonal Antibodies</title><source>MEDLINE</source><source>SAGE Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Sweetwyne, Mariya T ; Brekken, Rolf A ; Workman, Gail ; Bradshaw, Amy D ; Carbon, Juliet ; Siadak, Anthony W ; Murri, Carrie ; Sage, E. Helene</creator><creatorcontrib>Sweetwyne, Mariya T ; Brekken, Rolf A ; Workman, Gail ; Bradshaw, Amy D ; Carbon, Juliet ; Siadak, Anthony W ; Murri, Carrie ; Sage, E. Helene</creatorcontrib><description>SPARC (osteonectin, BM-40) is a matricellular glycoprotein that is expressed in many embryogenic and adult tissues undergoing remodeling or repair. SPARC modulates cellular interaction with the extracellular matrix (ECM), inhibits cell adhesion and proliferation, and regulates growth factor activity. To explore further the function and activity of this protein in tissue homeostasis, we have developed several monoclonal antibodies (MAbs) that recognize distinct epitopes on SPARC. The MAbs bind to SPARC with high affinity and identify SPARC by ELISA, Western blotting, immunoprecipitation, immunocytochemistry, and/or immunohistochemistry. The MAbs were also characterized in functional assays for potential alteration of SPARC activity. SPARC binds to collagen I and laminin-1 through an epitope defined by MAb 293; this epitope is not involved in the binding of SPARC to collagen III. The other MAbs did not interfere with the binding of SPARC to collagen I or III or laminin-1. Inhibition of the anti-adhesive effect of SPARC on endothelial cells by MAb 236 was also observed. Functional analysis of SPARC in the presence of these novel MAbs now confirms that the activities ascribed to this matricellular protein can be assigned to discrete subdomains.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1369/jhc.3A6153.2004</identifier><identifier>PMID: 15150281</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Animals ; Antibodies, Monoclonal ; Blotting, Western ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; Extracellular Matrix Proteins - immunology ; Extracellular Matrix Proteins - metabolism ; Humans ; Immunohistochemistry ; Male ; Mice ; Organ Specificity ; Osteonectin - immunology ; Osteonectin - metabolism ; Precipitin Tests ; Protein Binding</subject><ispartof>The journal of histochemistry and cytochemistry, 2004-06, Vol.52 (6), p.723-733</ispartof><rights>2004 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-1c6ace565c13a0bef85b09ad4def1ed861381c7942104e06a92293f36ef8fdef3</citedby><cites>FETCH-LOGICAL-c401t-1c6ace565c13a0bef85b09ad4def1ed861381c7942104e06a92293f36ef8fdef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1369/jhc.3A6153.2004$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1369/jhc.3A6153.2004$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,778,782,21802,27907,27908,43604,43605</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15150281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sweetwyne, Mariya T</creatorcontrib><creatorcontrib>Brekken, Rolf A</creatorcontrib><creatorcontrib>Workman, Gail</creatorcontrib><creatorcontrib>Bradshaw, Amy D</creatorcontrib><creatorcontrib>Carbon, Juliet</creatorcontrib><creatorcontrib>Siadak, Anthony W</creatorcontrib><creatorcontrib>Murri, Carrie</creatorcontrib><creatorcontrib>Sage, E. Helene</creatorcontrib><title>Functional Analysis of the Matricellular Protein SPARC with Novel Monoclonal Antibodies</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>SPARC (osteonectin, BM-40) is a matricellular glycoprotein that is expressed in many embryogenic and adult tissues undergoing remodeling or repair. SPARC modulates cellular interaction with the extracellular matrix (ECM), inhibits cell adhesion and proliferation, and regulates growth factor activity. To explore further the function and activity of this protein in tissue homeostasis, we have developed several monoclonal antibodies (MAbs) that recognize distinct epitopes on SPARC. The MAbs bind to SPARC with high affinity and identify SPARC by ELISA, Western blotting, immunoprecipitation, immunocytochemistry, and/or immunohistochemistry. The MAbs were also characterized in functional assays for potential alteration of SPARC activity. SPARC binds to collagen I and laminin-1 through an epitope defined by MAb 293; this epitope is not involved in the binding of SPARC to collagen III. The other MAbs did not interfere with the binding of SPARC to collagen I or III or laminin-1. Inhibition of the anti-adhesive effect of SPARC on endothelial cells by MAb 236 was also observed. Functional analysis of SPARC in the presence of these novel MAbs now confirms that the activities ascribed to this matricellular protein can be assigned to discrete subdomains.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Blotting, Western</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes</subject><subject>Extracellular Matrix Proteins - immunology</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mice</subject><subject>Organ Specificity</subject><subject>Osteonectin - immunology</subject><subject>Osteonectin - metabolism</subject><subject>Precipitin Tests</subject><subject>Protein Binding</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQQEVISbZJz70VXdJTvdHow2sflyVpC0kbmpYehVYex1q0ViLZNfn31eKF3noZwfD0GB4h74EtQZT19a6zS7EuQYklZ0yekAUoBYViUp6SBWOcF3khz8nblHaMgZSqOiPnoEAxXsGC_L4dezu40BtP13m8JpdoaOnQIb03Q3QWvR-9ifQhhgFdTx8f1j82dHJDR7-FP-jpfeiD9UfD4LahcZguyZvW-ITvju8F-XV783Pzpbj7_vnrZn1XWMlgKMCWxqIqlQVh2BbbSm1ZbRrZYAvYVCWICuyqlhyYRFaamvNatKLMZJsZcUE-zt7nGF5GTIPeu3S42fQYxqRXUCsGXGTwegZtDClFbPVzdHsTXzUwfWipc0s9t9SHlvnHh6N63O6x-ccf42Xg0wwk84R6F8aYG6T_-K5mvHNP3eQi6rQ33mc76GmaFNelXuVL_wKrp4r9</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Sweetwyne, Mariya T</creator><creator>Brekken, Rolf A</creator><creator>Workman, Gail</creator><creator>Bradshaw, Amy D</creator><creator>Carbon, Juliet</creator><creator>Siadak, Anthony W</creator><creator>Murri, Carrie</creator><creator>Sage, E. Helene</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040601</creationdate><title>Functional Analysis of the Matricellular Protein SPARC with Novel Monoclonal Antibodies</title><author>Sweetwyne, Mariya T ; Brekken, Rolf A ; Workman, Gail ; Bradshaw, Amy D ; Carbon, Juliet ; Siadak, Anthony W ; Murri, Carrie ; Sage, E. Helene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-1c6ace565c13a0bef85b09ad4def1ed861381c7942104e06a92293f36ef8fdef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Blotting, Western</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes</topic><topic>Extracellular Matrix Proteins - immunology</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Mice</topic><topic>Organ Specificity</topic><topic>Osteonectin - immunology</topic><topic>Osteonectin - metabolism</topic><topic>Precipitin Tests</topic><topic>Protein Binding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sweetwyne, Mariya T</creatorcontrib><creatorcontrib>Brekken, Rolf A</creatorcontrib><creatorcontrib>Workman, Gail</creatorcontrib><creatorcontrib>Bradshaw, Amy D</creatorcontrib><creatorcontrib>Carbon, Juliet</creatorcontrib><creatorcontrib>Siadak, Anthony W</creatorcontrib><creatorcontrib>Murri, Carrie</creatorcontrib><creatorcontrib>Sage, E. Helene</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sweetwyne, Mariya T</au><au>Brekken, Rolf A</au><au>Workman, Gail</au><au>Bradshaw, Amy D</au><au>Carbon, Juliet</au><au>Siadak, Anthony W</au><au>Murri, Carrie</au><au>Sage, E. Helene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Analysis of the Matricellular Protein SPARC with Novel Monoclonal Antibodies</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>52</volume><issue>6</issue><spage>723</spage><epage>733</epage><pages>723-733</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>SPARC (osteonectin, BM-40) is a matricellular glycoprotein that is expressed in many embryogenic and adult tissues undergoing remodeling or repair. SPARC modulates cellular interaction with the extracellular matrix (ECM), inhibits cell adhesion and proliferation, and regulates growth factor activity. To explore further the function and activity of this protein in tissue homeostasis, we have developed several monoclonal antibodies (MAbs) that recognize distinct epitopes on SPARC. The MAbs bind to SPARC with high affinity and identify SPARC by ELISA, Western blotting, immunoprecipitation, immunocytochemistry, and/or immunohistochemistry. The MAbs were also characterized in functional assays for potential alteration of SPARC activity. SPARC binds to collagen I and laminin-1 through an epitope defined by MAb 293; this epitope is not involved in the binding of SPARC to collagen III. The other MAbs did not interfere with the binding of SPARC to collagen I or III or laminin-1. Inhibition of the anti-adhesive effect of SPARC on endothelial cells by MAb 236 was also observed. Functional analysis of SPARC in the presence of these novel MAbs now confirms that the activities ascribed to this matricellular protein can be assigned to discrete subdomains.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>15150281</pmid><doi>10.1369/jhc.3A6153.2004</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1554 |
| ispartof | The journal of histochemistry and cytochemistry, 2004-06, Vol.52 (6), p.723-733 |
| issn | 0022-1554 1551-5044 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71950123 |
| source | MEDLINE; SAGE Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies, Monoclonal Blotting, Western Enzyme-Linked Immunosorbent Assay Epitopes Extracellular Matrix Proteins - immunology Extracellular Matrix Proteins - metabolism Humans Immunohistochemistry Male Mice Organ Specificity Osteonectin - immunology Osteonectin - metabolism Precipitin Tests Protein Binding |
| title | Functional Analysis of the Matricellular Protein SPARC with Novel Monoclonal Antibodies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T05%3A15%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20Analysis%20of%20the%20Matricellular%20Protein%20SPARC%20with%20Novel%20Monoclonal%20Antibodies&rft.jtitle=The%20journal%20of%20histochemistry%20and%20cytochemistry&rft.au=Sweetwyne,%20Mariya%20T&rft.date=2004-06-01&rft.volume=52&rft.issue=6&rft.spage=723&rft.epage=733&rft.pages=723-733&rft.issn=0022-1554&rft.eissn=1551-5044&rft_id=info:doi/10.1369/jhc.3A6153.2004&rft_dat=%3Cproquest_cross%3E71950123%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71950123&rft_id=info:pmid/15150281&rft_sage_id=10.1369_jhc.3A6153.2004&rfr_iscdi=true |