Expresssion of Bcl-2 oncoprotein in cases of acute and chronic viral hepatitis type B and type C: A clinicopathologic study
The bcl-2 gene is involved in the regulation of programmed cell death by providing a survival advantage to rapidly proliferating cells. This study investigates bcl-2 protein expression in liver biopsies with acute or chronic viral hepatitis B (HBV) or C(HCV). The study comprised 60 liver biopsies wi...
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| Veröffentlicht in: | Digestive diseases and sciences 2002-07, Vol.47 (7), p.1618-1624 |
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| creator | TSAMANDAS, Athanassios C THOMOPOULOS, Konstantinos GOGOS, Charalambos TEPETES, Konstantinos KOURELIS, Theodore RAVAZOULA, Panagiota PETSAS, Theodore BONIKOS, Dionissis S KARATZA, Chrisoula KARAVIAS, Dionissios D |
| description | The bcl-2 gene is involved in the regulation of programmed cell death by providing a survival advantage to rapidly proliferating cells. This study investigates bcl-2 protein expression in liver biopsies with acute or chronic viral hepatitis B (HBV) or C(HCV). The study comprised 60 liver biopsies with hepatitis B or C. These included 10 biopsies from cases with acute lobular hepatitis (ALH), and 50 with chronic hepatitis (CH). In addition, 10 liver biopsies were used as controls. In CH cases, HAI grade ranged from 3/18 to 15/18, and stage from 1 to 6 (6HBV/8HCV). Immunohistochemical bcl-2 expression was assessed using the streptavidin-biotin method and the presence of apoptotic cells was evaluated by TUNEL method. Immunohistochemical and in situ hybridization (TUNEL) results were expressed following morphometric analysis. In CH cases, bcl-2 was detected in portal and intralobular lymphocytes, and in cholangiolar epithelial cells of the interface area. In ALH and control cases, bcl-2 was expressed only in lymphocytes. Lymphocytic bcl-2 expression was correlated directly with categories A, C and D of HAI (P < 0.001), whereas the degree of fibrosis was reversibly correlated to bcl-2 expression. In conclusion, in cases of chronic hepatitis, bcl-2 expression in cholangioles of interface area suggests that this oncoprotein may be involved in regulation of growth of these epithelial cells. |
| doi_str_mv | 10.1023/A:1015839807627 |
| format | Article |
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This study investigates bcl-2 protein expression in liver biopsies with acute or chronic viral hepatitis B (HBV) or C(HCV). The study comprised 60 liver biopsies with hepatitis B or C. These included 10 biopsies from cases with acute lobular hepatitis (ALH), and 50 with chronic hepatitis (CH). In addition, 10 liver biopsies were used as controls. In CH cases, HAI grade ranged from 3/18 to 15/18, and stage from 1 to 6 (6HBV/8HCV). Immunohistochemical bcl-2 expression was assessed using the streptavidin-biotin method and the presence of apoptotic cells was evaluated by TUNEL method. Immunohistochemical and in situ hybridization (TUNEL) results were expressed following morphometric analysis. In CH cases, bcl-2 was detected in portal and intralobular lymphocytes, and in cholangiolar epithelial cells of the interface area. In ALH and control cases, bcl-2 was expressed only in lymphocytes. Lymphocytic bcl-2 expression was correlated directly with categories A, C and D of HAI (P < 0.001), whereas the degree of fibrosis was reversibly correlated to bcl-2 expression. In conclusion, in cases of chronic hepatitis, bcl-2 expression in cholangioles of interface area suggests that this oncoprotein may be involved in regulation of growth of these epithelial cells.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1023/A:1015839807627</identifier><identifier>PMID: 12141825</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Adult ; Aged ; Apoptosis ; Bile Ducts, Intrahepatic - metabolism ; Biological and medical sciences ; Female ; Hepatitis B, Chronic - metabolism ; Hepatitis C, Chronic - metabolism ; Human viral diseases ; Humans ; Immunohistochemistry ; In Situ Nick-End Labeling ; Infectious diseases ; Liver - metabolism ; Male ; Medical sciences ; Middle Aged ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Viral diseases ; Viral hepatitis</subject><ispartof>Digestive diseases and sciences, 2002-07, Vol.47 (7), p.1618-1624</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright Kluwer Academic Publishers Jul 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13819215$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12141825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TSAMANDAS, Athanassios C</creatorcontrib><creatorcontrib>THOMOPOULOS, Konstantinos</creatorcontrib><creatorcontrib>GOGOS, Charalambos</creatorcontrib><creatorcontrib>TEPETES, Konstantinos</creatorcontrib><creatorcontrib>KOURELIS, Theodore</creatorcontrib><creatorcontrib>RAVAZOULA, Panagiota</creatorcontrib><creatorcontrib>PETSAS, Theodore</creatorcontrib><creatorcontrib>BONIKOS, Dionissis S</creatorcontrib><creatorcontrib>KARATZA, Chrisoula</creatorcontrib><creatorcontrib>KARAVIAS, Dionissios D</creatorcontrib><title>Expresssion of Bcl-2 oncoprotein in cases of acute and chronic viral hepatitis type B and type C: A clinicopathologic study</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><description>The bcl-2 gene is involved in the regulation of programmed cell death by providing a survival advantage to rapidly proliferating cells. This study investigates bcl-2 protein expression in liver biopsies with acute or chronic viral hepatitis B (HBV) or C(HCV). The study comprised 60 liver biopsies with hepatitis B or C. These included 10 biopsies from cases with acute lobular hepatitis (ALH), and 50 with chronic hepatitis (CH). In addition, 10 liver biopsies were used as controls. In CH cases, HAI grade ranged from 3/18 to 15/18, and stage from 1 to 6 (6HBV/8HCV). Immunohistochemical bcl-2 expression was assessed using the streptavidin-biotin method and the presence of apoptotic cells was evaluated by TUNEL method. Immunohistochemical and in situ hybridization (TUNEL) results were expressed following morphometric analysis. In CH cases, bcl-2 was detected in portal and intralobular lymphocytes, and in cholangiolar epithelial cells of the interface area. In ALH and control cases, bcl-2 was expressed only in lymphocytes. Lymphocytic bcl-2 expression was correlated directly with categories A, C and D of HAI (P < 0.001), whereas the degree of fibrosis was reversibly correlated to bcl-2 expression. In conclusion, in cases of chronic hepatitis, bcl-2 expression in cholangioles of interface area suggests that this oncoprotein may be involved in regulation of growth of these epithelial cells.</description><subject>Adult</subject><subject>Aged</subject><subject>Apoptosis</subject><subject>Bile Ducts, Intrahepatic - metabolism</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Hepatitis B, Chronic - metabolism</subject><subject>Hepatitis C, Chronic - metabolism</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Nick-End Labeling</subject><subject>Infectious diseases</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0N9L5DAQB_AgyrnqPfsmQTjf6mWS5kd9Wxf1DgRfzucymyZupNvUppVb_OeNuiIIgRmYT4bkS8gxsHNgXPyeXwADaURlmFZc75AZSC0KLpXZJTMGKvcAap8cpPTIGKs0qB9kHziUYLickZer__3gUkohdjR6emnbgtPY2dgPcXSho_lYTC69TdFOo6PYNdSuhtgFS5_DgC1duR7HMIZEx03v6OU7eW8XF3RObRuyjdmsYhsf8rU0Ts3miOx5bJP7ua2H5P766t_iT3F7d_N3Mb8teq7KsTB8WeoGK6M0Q1MayY23rvQeJC5VBZpLbhnKyjdu6YEpxtFr6T2K0uYExCE5-9ibv_Q0uTTW65Csa1vsXJxSraEqtWEsw9Nv8DFOQ5ffVufAhGBg3radbNG0XLum7oewxmFTf2aawa8twGSx9QN2NqQvJwxUHKR4BTIxhSU</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>TSAMANDAS, Athanassios C</creator><creator>THOMOPOULOS, Konstantinos</creator><creator>GOGOS, Charalambos</creator><creator>TEPETES, Konstantinos</creator><creator>KOURELIS, Theodore</creator><creator>RAVAZOULA, Panagiota</creator><creator>PETSAS, Theodore</creator><creator>BONIKOS, Dionissis S</creator><creator>KARATZA, Chrisoula</creator><creator>KARAVIAS, Dionissios D</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Expresssion of Bcl-2 oncoprotein in cases of acute and chronic viral hepatitis type B and type C: A clinicopathologic study</title><author>TSAMANDAS, Athanassios C ; THOMOPOULOS, Konstantinos ; GOGOS, Charalambos ; TEPETES, Konstantinos ; KOURELIS, Theodore ; RAVAZOULA, Panagiota ; PETSAS, Theodore ; BONIKOS, Dionissis S ; KARATZA, Chrisoula ; KARAVIAS, Dionissios D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p264t-82b47da98670a848528fce4ff15ab6917252c0a59fdebf10602af75ffa34c1633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Apoptosis</topic><topic>Bile Ducts, Intrahepatic - metabolism</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Hepatitis B, Chronic - metabolism</topic><topic>Hepatitis C, Chronic - metabolism</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Nick-End Labeling</topic><topic>Infectious diseases</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TSAMANDAS, Athanassios C</creatorcontrib><creatorcontrib>THOMOPOULOS, Konstantinos</creatorcontrib><creatorcontrib>GOGOS, Charalambos</creatorcontrib><creatorcontrib>TEPETES, Konstantinos</creatorcontrib><creatorcontrib>KOURELIS, Theodore</creatorcontrib><creatorcontrib>RAVAZOULA, Panagiota</creatorcontrib><creatorcontrib>PETSAS, Theodore</creatorcontrib><creatorcontrib>BONIKOS, Dionissis S</creatorcontrib><creatorcontrib>KARATZA, Chrisoula</creatorcontrib><creatorcontrib>KARAVIAS, Dionissios D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TSAMANDAS, Athanassios C</au><au>THOMOPOULOS, Konstantinos</au><au>GOGOS, Charalambos</au><au>TEPETES, Konstantinos</au><au>KOURELIS, Theodore</au><au>RAVAZOULA, Panagiota</au><au>PETSAS, Theodore</au><au>BONIKOS, Dionissis S</au><au>KARATZA, Chrisoula</au><au>KARAVIAS, Dionissios D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expresssion of Bcl-2 oncoprotein in cases of acute and chronic viral hepatitis type B and type C: A clinicopathologic study</atitle><jtitle>Digestive diseases and sciences</jtitle><addtitle>Dig Dis Sci</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>47</volume><issue>7</issue><spage>1618</spage><epage>1624</epage><pages>1618-1624</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract>The bcl-2 gene is involved in the regulation of programmed cell death by providing a survival advantage to rapidly proliferating cells. This study investigates bcl-2 protein expression in liver biopsies with acute or chronic viral hepatitis B (HBV) or C(HCV). The study comprised 60 liver biopsies with hepatitis B or C. These included 10 biopsies from cases with acute lobular hepatitis (ALH), and 50 with chronic hepatitis (CH). In addition, 10 liver biopsies were used as controls. In CH cases, HAI grade ranged from 3/18 to 15/18, and stage from 1 to 6 (6HBV/8HCV). Immunohistochemical bcl-2 expression was assessed using the streptavidin-biotin method and the presence of apoptotic cells was evaluated by TUNEL method. Immunohistochemical and in situ hybridization (TUNEL) results were expressed following morphometric analysis. In CH cases, bcl-2 was detected in portal and intralobular lymphocytes, and in cholangiolar epithelial cells of the interface area. In ALH and control cases, bcl-2 was expressed only in lymphocytes. Lymphocytic bcl-2 expression was correlated directly with categories A, C and D of HAI (P < 0.001), whereas the degree of fibrosis was reversibly correlated to bcl-2 expression. In conclusion, in cases of chronic hepatitis, bcl-2 expression in cholangioles of interface area suggests that this oncoprotein may be involved in regulation of growth of these epithelial cells.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>12141825</pmid><doi>10.1023/A:1015839807627</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged Apoptosis Bile Ducts, Intrahepatic - metabolism Biological and medical sciences Female Hepatitis B, Chronic - metabolism Hepatitis C, Chronic - metabolism Human viral diseases Humans Immunohistochemistry In Situ Nick-End Labeling Infectious diseases Liver - metabolism Male Medical sciences Middle Aged Proto-Oncogene Proteins c-bcl-2 - metabolism Viral diseases Viral hepatitis |
| title | Expresssion of Bcl-2 oncoprotein in cases of acute and chronic viral hepatitis type B and type C: A clinicopathologic study |
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