Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions
A combined bioreactor system, composed of a stirred tank and a three-stage tubular bioreactor in series and with a total working volume of 3260 ml, was established. Continuous ethanol production was carried out using Saccharomyces cerevisiae and a very high gravity (VHG) medium containing 280 g l −1...
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| Veröffentlicht in: | Journal of biotechnology 2004-06, Vol.110 (3), p.287-293 |
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| creator | Bai, F.W Chen, L.J Zhang, Z Anderson, W.A Moo-Young, M |
| description | A combined bioreactor system, composed of a stirred tank and a three-stage tubular bioreactor in series and with a total working volume of 3260
ml, was established. Continuous ethanol production was carried out using
Saccharomyces cerevisiae and a very high gravity (VHG) medium containing 280
g
l
−1 glucose. An average ethanol concentration of 124.6
g
l
−1 or 15.8% (v) was produced when the bioreactor system was operated at a dilution rate of 0.012
h
−1. The yield of ethanol to glucose consumed was calculated to be 0.484 or 94.7% of its theoretical value of 0.511 when ethanol entrapped in the exhaust gas was incorporated. Meanwhile, quasi-steady states and non-steady oscillations were observed for residual glucose, ethanol and biomass concentrations for all of these bioreactors during their operations. Models that can be used to predict yeast cell lysis and viability loss were developed. |
| doi_str_mv | 10.1016/j.jbiotec.2004.01.017 |
| format | Article |
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ml, was established. Continuous ethanol production was carried out using
Saccharomyces cerevisiae and a very high gravity (VHG) medium containing 280
g
l
−1 glucose. An average ethanol concentration of 124.6
g
l
−1 or 15.8% (v) was produced when the bioreactor system was operated at a dilution rate of 0.012
h
−1. The yield of ethanol to glucose consumed was calculated to be 0.484 or 94.7% of its theoretical value of 0.511 when ethanol entrapped in the exhaust gas was incorporated. Meanwhile, quasi-steady states and non-steady oscillations were observed for residual glucose, ethanol and biomass concentrations for all of these bioreactors during their operations. Models that can be used to predict yeast cell lysis and viability loss were developed.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/j.jbiotec.2004.01.017</identifier><identifier>PMID: 15163519</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biomass ; Bioreactors ; Biotechnology ; Cell lysis and viability loss ; Cell Physiological Phenomena ; Combined bioreactor system ; continuous fermentation ; Culture Media - chemistry ; Ethanol - analysis ; Ethanol - metabolism ; Ethanol production ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Glucose - analysis ; Glucose - metabolism ; Gravitation ; Kinetics ; Models, Biological ; Peptones - chemistry ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - metabolism ; Very high gravity medium ; yeasts</subject><ispartof>Journal of biotechnology, 2004-06, Vol.110 (3), p.287-293</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-967287ed12936a2ffd88861b4b1b71cfb379910cb344670b4cea03b382d097c13</citedby><cites>FETCH-LOGICAL-c549t-967287ed12936a2ffd88861b4b1b71cfb379910cb344670b4cea03b382d097c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiotec.2004.01.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15795642$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15163519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bai, F.W</creatorcontrib><creatorcontrib>Chen, L.J</creatorcontrib><creatorcontrib>Zhang, Z</creatorcontrib><creatorcontrib>Anderson, W.A</creatorcontrib><creatorcontrib>Moo-Young, M</creatorcontrib><title>Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>A combined bioreactor system, composed of a stirred tank and a three-stage tubular bioreactor in series and with a total working volume of 3260
ml, was established. Continuous ethanol production was carried out using
Saccharomyces cerevisiae and a very high gravity (VHG) medium containing 280
g
l
−1 glucose. An average ethanol concentration of 124.6
g
l
−1 or 15.8% (v) was produced when the bioreactor system was operated at a dilution rate of 0.012
h
−1. The yield of ethanol to glucose consumed was calculated to be 0.484 or 94.7% of its theoretical value of 0.511 when ethanol entrapped in the exhaust gas was incorporated. Meanwhile, quasi-steady states and non-steady oscillations were observed for residual glucose, ethanol and biomass concentrations for all of these bioreactors during their operations. Models that can be used to predict yeast cell lysis and viability loss were developed.</description><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell lysis and viability loss</subject><subject>Cell Physiological Phenomena</subject><subject>Combined bioreactor system</subject><subject>continuous fermentation</subject><subject>Culture Media - chemistry</subject><subject>Ethanol - analysis</subject><subject>Ethanol - metabolism</subject><subject>Ethanol production</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - analysis</subject><subject>Glucose - metabolism</subject><subject>Gravitation</subject><subject>Kinetics</subject><subject>Models, Biological</subject><subject>Peptones - chemistry</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Very high gravity medium</subject><subject>yeasts</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxRtR3NnVj6DmorcZU510_pxEBl2FBQ-655Ck0zMZepI16W7og9_d9E6D3hYKQsivXuXVq6o3gHeAgX087U7Gx8HZXY0x3WEoxZ9VGxCcbKlg5Hm1KZzYAmvYVXWd8wkXUDbwsrqCBhhpQG6qP_sYBh_GOGbkhqMOsUcPKbajHXwMSIcWuUn3o368xg7NTucBWdf3qJ-zz4_I5LXxvR9m1Mec0Rhal9Dk0oyO_nBEh6Sn5fHsWj-ekY2h9YteflW96HSf3ev1vKnuv375tf-2vftx-33_-W5rGyqHrWS8Fty1UEvCdN11rRCCgaEGDAfbGcKlBGwNoZRxbKh1GhNDRN1iyS2Qm-rDRbdY-z26PKizz4sHHVxxrjhIypkgT4LAJaeNkAVsLqBNxXFynXpI_qzTrACrJSB1UmtAaglIYSjFS9_bdcBoyjr-da2JFOD9Cuhsdd8lHazP_3FcNozWhXt34TodlT6kwtz_rDEQjCVpKF6UPl0IVzY7eZdUtt4FW0JIzg6qjf6Jz_4Fe828jg</recordid><startdate>20040610</startdate><enddate>20040610</enddate><creator>Bai, F.W</creator><creator>Chen, L.J</creator><creator>Zhang, Z</creator><creator>Anderson, W.A</creator><creator>Moo-Young, M</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040610</creationdate><title>Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions</title><author>Bai, F.W ; Chen, L.J ; Zhang, Z ; Anderson, W.A ; Moo-Young, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-967287ed12936a2ffd88861b4b1b71cfb379910cb344670b4cea03b382d097c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Cell lysis and viability loss</topic><topic>Cell Physiological Phenomena</topic><topic>Combined bioreactor system</topic><topic>continuous fermentation</topic><topic>Culture Media - chemistry</topic><topic>Ethanol - analysis</topic><topic>Ethanol - metabolism</topic><topic>Ethanol production</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - analysis</topic><topic>Glucose - metabolism</topic><topic>Gravitation</topic><topic>Kinetics</topic><topic>Models, Biological</topic><topic>Peptones - chemistry</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Very high gravity medium</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bai, F.W</creatorcontrib><creatorcontrib>Chen, L.J</creatorcontrib><creatorcontrib>Zhang, Z</creatorcontrib><creatorcontrib>Anderson, W.A</creatorcontrib><creatorcontrib>Moo-Young, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bai, F.W</au><au>Chen, L.J</au><au>Zhang, Z</au><au>Anderson, W.A</au><au>Moo-Young, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2004-06-10</date><risdate>2004</risdate><volume>110</volume><issue>3</issue><spage>287</spage><epage>293</epage><pages>287-293</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>A combined bioreactor system, composed of a stirred tank and a three-stage tubular bioreactor in series and with a total working volume of 3260
ml, was established. Continuous ethanol production was carried out using
Saccharomyces cerevisiae and a very high gravity (VHG) medium containing 280
g
l
−1 glucose. An average ethanol concentration of 124.6
g
l
−1 or 15.8% (v) was produced when the bioreactor system was operated at a dilution rate of 0.012
h
−1. The yield of ethanol to glucose consumed was calculated to be 0.484 or 94.7% of its theoretical value of 0.511 when ethanol entrapped in the exhaust gas was incorporated. Meanwhile, quasi-steady states and non-steady oscillations were observed for residual glucose, ethanol and biomass concentrations for all of these bioreactors during their operations. Models that can be used to predict yeast cell lysis and viability loss were developed.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15163519</pmid><doi>10.1016/j.jbiotec.2004.01.017</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of biotechnology, 2004-06, Vol.110 (3), p.287-293 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences Biomass Bioreactors Biotechnology Cell lysis and viability loss Cell Physiological Phenomena Combined bioreactor system continuous fermentation Culture Media - chemistry Ethanol - analysis Ethanol - metabolism Ethanol production Fermentation Fundamental and applied biological sciences. Psychology Glucose - analysis Glucose - metabolism Gravitation Kinetics Models, Biological Peptones - chemistry Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Very high gravity medium yeasts |
| title | Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions |
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