Analysis of organophosphorus compound adducts of serine proteases by liquid chromatography–tandem mass spectrometry

In order to confirm that diisopropylfluorophosphate (DFP) phosphorylates the active site serine residue in α-chymotrypsin, a peptide containing the phosphorylated active site was analyzed by liquid chromatography (LC)–electrospray mass spectrometry (ESI-MS). After reduction with dithiothreitol and s...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2002-08, Vol.776 (1), p.79-88
Hauptverfasser:	Tsuge, Kouichiro, Seto, Yasuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography, Liquid - methods Molecular Sequence Data Organophosphorus Organophosphorus Compounds - analysis Peptide Fragments - chemistry Serine Endopeptidases - chemistry Serine protease Spectrometry, Mass, Electrospray Ionization - methods
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