The Neurospora crassa pheromone precursor genes are regulated by the mating type locus and the circadian clock
Summary Pheromones play important roles in female and male behaviour in the filamentous ascomycete fungi. To begin to explore the role of pheromones in mating, we have identified the genes encoding the sex pheromones of the heterothallic species Neurospora crassa. One gene, expressed exclusively in...
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| Veröffentlicht in: | Molecular microbiology 2002-08, Vol.45 (3), p.795-804 |
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| creator | Bobrowicz, Piotr Pawlak, Rebecca Correa, Alejandro Bell‐Pedersen, Deborah Ebbole, Daniel J. |
| description | Summary
Pheromones play important roles in female and male behaviour in the filamentous ascomycete fungi. To begin to explore the role of pheromones in mating, we have identified the genes encoding the sex pheromones of the heterothallic species Neurospora crassa. One gene, expressed exclusively in mat A strains, encodes a polypeptide containing multiple repeats of a putative pheromone sequence bordered by Kex2 processing sites. Strains of the opposite mating type, mat a, express a pheromone precursor gene whose polypeptide contains a C‐terminal CAAX motif predicted to produce a mature pheromone with a C‐terminal carboxy‐methyl isoprenylated cysteine. The predicted sequences of the pheromones are remarkably similar to those encoded by other filamentous ascomycetes. The expression of the pheromone precursor genes is mating type specific and is under the control of the mating type locus. Furthermore, the genes are highly expressed in conidia and under conditions that favour sexual development. Both pheromone precursor genes are also regulated by the endogenous circadian clock in a time‐of‐day‐specific fashion, supporting a role for the clock in mating. |
| doi_str_mv | 10.1046/j.1365-2958.2002.03052.x |
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Pheromones play important roles in female and male behaviour in the filamentous ascomycete fungi. To begin to explore the role of pheromones in mating, we have identified the genes encoding the sex pheromones of the heterothallic species Neurospora crassa. One gene, expressed exclusively in mat A strains, encodes a polypeptide containing multiple repeats of a putative pheromone sequence bordered by Kex2 processing sites. Strains of the opposite mating type, mat a, express a pheromone precursor gene whose polypeptide contains a C‐terminal CAAX motif predicted to produce a mature pheromone with a C‐terminal carboxy‐methyl isoprenylated cysteine. The predicted sequences of the pheromones are remarkably similar to those encoded by other filamentous ascomycetes. The expression of the pheromone precursor genes is mating type specific and is under the control of the mating type locus. Furthermore, the genes are highly expressed in conidia and under conditions that favour sexual development. Both pheromone precursor genes are also regulated by the endogenous circadian clock in a time‐of‐day‐specific fashion, supporting a role for the clock in mating.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2002.03052.x</identifier><identifier>PMID: 12139624</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Amino Acid Sequence ; Biological Clocks ; Circadian Rhythm - physiology ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Expression Regulation, Fungal ; Mating Factor ; Molecular Sequence Data ; Morphogenesis - genetics ; Neurospora crassa - genetics ; Neurospora crassa - growth & development ; Neurospora crassa - physiology ; Peptides - physiology ; Pheromones - genetics ; Pheromones - metabolism ; Pheromones - physiology ; Protein Precursors - chemistry ; Protein Precursors - genetics ; Protein Precursors - metabolism ; Restriction Mapping ; Sequence Homology, Amino Acid</subject><ispartof>Molecular microbiology, 2002-08, Vol.45 (3), p.795-804</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. Aug 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5392-b9d8a849e8a2e1e0986ca9dcaf82f872d9a5b14e10339d2eaad474532aeb5dd3</citedby><cites>FETCH-LOGICAL-c5392-b9d8a849e8a2e1e0986ca9dcaf82f872d9a5b14e10339d2eaad474532aeb5dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.2002.03052.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.2002.03052.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12139624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bobrowicz, Piotr</creatorcontrib><creatorcontrib>Pawlak, Rebecca</creatorcontrib><creatorcontrib>Correa, Alejandro</creatorcontrib><creatorcontrib>Bell‐Pedersen, Deborah</creatorcontrib><creatorcontrib>Ebbole, Daniel J.</creatorcontrib><title>The Neurospora crassa pheromone precursor genes are regulated by the mating type locus and the circadian clock</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Pheromones play important roles in female and male behaviour in the filamentous ascomycete fungi. To begin to explore the role of pheromones in mating, we have identified the genes encoding the sex pheromones of the heterothallic species Neurospora crassa. One gene, expressed exclusively in mat A strains, encodes a polypeptide containing multiple repeats of a putative pheromone sequence bordered by Kex2 processing sites. Strains of the opposite mating type, mat a, express a pheromone precursor gene whose polypeptide contains a C‐terminal CAAX motif predicted to produce a mature pheromone with a C‐terminal carboxy‐methyl isoprenylated cysteine. The predicted sequences of the pheromones are remarkably similar to those encoded by other filamentous ascomycetes. The expression of the pheromone precursor genes is mating type specific and is under the control of the mating type locus. Furthermore, the genes are highly expressed in conidia and under conditions that favour sexual development. Both pheromone precursor genes are also regulated by the endogenous circadian clock in a time‐of‐day‐specific fashion, supporting a role for the clock in mating.</description><subject>Amino Acid Sequence</subject><subject>Biological Clocks</subject><subject>Circadian Rhythm - physiology</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Mating Factor</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis - genetics</subject><subject>Neurospora crassa - genetics</subject><subject>Neurospora crassa - growth & development</subject><subject>Neurospora crassa - physiology</subject><subject>Peptides - physiology</subject><subject>Pheromones - genetics</subject><subject>Pheromones - metabolism</subject><subject>Pheromones - physiology</subject><subject>Protein Precursors - chemistry</subject><subject>Protein Precursors - genetics</subject><subject>Protein Precursors - metabolism</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Amino Acid</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctuFDEQRS1ERIbALyCLBbtu_OyxFyxQxCNSHptZZGdV2zWTHvqF3a1k_h53ZgQSG1iVpXvqSuVDCOWs5ExVH_cll5UuhNWmFIyJkkmmRfn0gqx-By_JilnNCmnE_Tl5ndKeMS5ZJV-Rcy64tJVQK9JvHpDe4hyHNA4RqI-QEtDxAePQDT3SMaKfYxoi3WGPiUJEGnE3tzBhoPWBTrmgg6npd3Q6jEjbwc8Z68Nz4pvoITTQU5-DH2_I2RbahG9P84Jsvn7ZXH4vru--XV1-vi68llYUtQ0GjLJoQCBHZk3lwQYPWyO2Zi2CBV1zhZxJaYNAgKDWSksBWOsQ5AX5cKwd4_BzxjS5rkke2xZ6HObk1tyqihv7T5AblWutyuD7v8D9MMc-3-C4rbTQa2syZI6Qz9-ZIm7dGJsO4sFx5hZxbu8WP27x4xZx7lmce8qr7079c91h-LN4MpWBT0fgsWnx8N_F7ubmannJXzfPqCM</recordid><startdate>200208</startdate><enddate>200208</enddate><creator>Bobrowicz, Piotr</creator><creator>Pawlak, Rebecca</creator><creator>Correa, Alejandro</creator><creator>Bell‐Pedersen, Deborah</creator><creator>Ebbole, Daniel J.</creator><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200208</creationdate><title>The Neurospora crassa pheromone precursor genes are regulated by the mating type locus and the circadian clock</title><author>Bobrowicz, Piotr ; Pawlak, Rebecca ; Correa, Alejandro ; Bell‐Pedersen, Deborah ; Ebbole, Daniel J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5392-b9d8a849e8a2e1e0986ca9dcaf82f872d9a5b14e10339d2eaad474532aeb5dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Biological Clocks</topic><topic>Circadian Rhythm - physiology</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Mating Factor</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis - genetics</topic><topic>Neurospora crassa - genetics</topic><topic>Neurospora crassa - growth & development</topic><topic>Neurospora crassa - physiology</topic><topic>Peptides - physiology</topic><topic>Pheromones - genetics</topic><topic>Pheromones - metabolism</topic><topic>Pheromones - physiology</topic><topic>Protein Precursors - chemistry</topic><topic>Protein Precursors - genetics</topic><topic>Protein Precursors - metabolism</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bobrowicz, Piotr</creatorcontrib><creatorcontrib>Pawlak, Rebecca</creatorcontrib><creatorcontrib>Correa, Alejandro</creatorcontrib><creatorcontrib>Bell‐Pedersen, Deborah</creatorcontrib><creatorcontrib>Ebbole, Daniel J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bobrowicz, Piotr</au><au>Pawlak, Rebecca</au><au>Correa, Alejandro</au><au>Bell‐Pedersen, Deborah</au><au>Ebbole, Daniel J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Neurospora crassa pheromone precursor genes are regulated by the mating type locus and the circadian clock</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2002-08</date><risdate>2002</risdate><volume>45</volume><issue>3</issue><spage>795</spage><epage>804</epage><pages>795-804</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Pheromones play important roles in female and male behaviour in the filamentous ascomycete fungi. To begin to explore the role of pheromones in mating, we have identified the genes encoding the sex pheromones of the heterothallic species Neurospora crassa. One gene, expressed exclusively in mat A strains, encodes a polypeptide containing multiple repeats of a putative pheromone sequence bordered by Kex2 processing sites. Strains of the opposite mating type, mat a, express a pheromone precursor gene whose polypeptide contains a C‐terminal CAAX motif predicted to produce a mature pheromone with a C‐terminal carboxy‐methyl isoprenylated cysteine. The predicted sequences of the pheromones are remarkably similar to those encoded by other filamentous ascomycetes. The expression of the pheromone precursor genes is mating type specific and is under the control of the mating type locus. Furthermore, the genes are highly expressed in conidia and under conditions that favour sexual development. Both pheromone precursor genes are also regulated by the endogenous circadian clock in a time‐of‐day‐specific fashion, supporting a role for the clock in mating.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12139624</pmid><doi>10.1046/j.1365-2958.2002.03052.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Biological Clocks Circadian Rhythm - physiology Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - metabolism Gene Expression Regulation, Fungal Mating Factor Molecular Sequence Data Morphogenesis - genetics Neurospora crassa - genetics Neurospora crassa - growth & development Neurospora crassa - physiology Peptides - physiology Pheromones - genetics Pheromones - metabolism Pheromones - physiology Protein Precursors - chemistry Protein Precursors - genetics Protein Precursors - metabolism Restriction Mapping Sequence Homology, Amino Acid |
| title | The Neurospora crassa pheromone precursor genes are regulated by the mating type locus and the circadian clock |
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