Utility of Rapidly Matured Oocytes as Recipients for Production of Cloned Embryos from Somatic Cells in the Pig
The present study was conducted to examine the utility of rapidly matured oocytes as recipients for production of porcine embryos reconstituted with adult skin fibroblasts and whether arrest of meiotic resumption of recipient oocytes at the germinal vesicle (GV) stage by dibutyryl cyclic AMP (dbcAMP...
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| Veröffentlicht in: | Biology of reproduction 2002-08, Vol.67 (2), p.540-545 |
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| creator | MIYOSHI, Kazuchika RZUCIDLO, S. Jacek PRATT, Scott L STICE, Steven L |
| description | The present study was conducted to examine the utility of rapidly matured oocytes as recipients for production of porcine
embryos reconstituted with adult skin fibroblasts and whether arrest of meiotic resumption of recipient oocytes at the germinal
vesicle (GV) stage by dibutyryl cyclic AMP (dbcAMP) improves in vitro developmental rates after reconstruction. At 24 h of
maturation in the medium, 36.3% of oocytes reached the metaphase II (MII) stage. At 30 h of maturation, the percentage (71.4%)
of MII oocytes did not significantly differ from that (78.0%) at 42 h of maturation. When MII oocytes recovered at 24 h of
maturation were used as recipients, 22/156 (14.1%) cloned embryos developing to the blastocyst stage was significantly ( P < 0.05) higher than those of embryos reconstituted with oocytes collected at 30 h (5/168; 3.0%) and 42 h (13/217; 6.0%) of
maturation. Culture of oocytes in medium containing 1 mM dbcAMP for 20 h maintained 72.9% in the GV stage, whereas only 15.0%
of nontreated oocytes were in the GV stage ( P < 0.05). The effect of dbcAMP was reversible. However, the treatment of recipient oocytes with dbcAMP did not affect the
development of reconstructed embryos when compared with nontreated oocytes. These results indicate that rapidly matured oocytes
are superior in their ability to support development of porcine reconstructed embryos; however, arrest of meiotic resumption
of recipient oocytes at the GV stage by dbcAMP does not improve reconstructed embryo developmental rates. |
| doi_str_mv | 10.1095/biolreprod67.2.540 |
| format | Article |
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embryos reconstituted with adult skin fibroblasts and whether arrest of meiotic resumption of recipient oocytes at the germinal
vesicle (GV) stage by dibutyryl cyclic AMP (dbcAMP) improves in vitro developmental rates after reconstruction. At 24 h of
maturation in the medium, 36.3% of oocytes reached the metaphase II (MII) stage. At 30 h of maturation, the percentage (71.4%)
of MII oocytes did not significantly differ from that (78.0%) at 42 h of maturation. When MII oocytes recovered at 24 h of
maturation were used as recipients, 22/156 (14.1%) cloned embryos developing to the blastocyst stage was significantly ( P < 0.05) higher than those of embryos reconstituted with oocytes collected at 30 h (5/168; 3.0%) and 42 h (13/217; 6.0%) of
maturation. Culture of oocytes in medium containing 1 mM dbcAMP for 20 h maintained 72.9% in the GV stage, whereas only 15.0%
of nontreated oocytes were in the GV stage ( P < 0.05). The effect of dbcAMP was reversible. However, the treatment of recipient oocytes with dbcAMP did not affect the
development of reconstructed embryos when compared with nontreated oocytes. These results indicate that rapidly matured oocytes
are superior in their ability to support development of porcine reconstructed embryos; however, arrest of meiotic resumption
of recipient oocytes at the GV stage by dbcAMP does not improve reconstructed embryo developmental rates.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod67.2.540</identifier><identifier>PMID: 12135893</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Bucladesine - pharmacology ; Cloning, Organism - methods ; Ear, External - cytology ; Ear, External - physiology ; Embryo Transfer ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fibroblasts - physiology ; Fundamental and applied biological sciences. Psychology ; Germinal Center - physiology ; Metaphase - physiology ; Molecular embryology ; Oocytes - physiology ; Pregnancy ; Swine</subject><ispartof>Biology of reproduction, 2002-08, Vol.67 (2), p.540-545</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13819319$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12135893$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MIYOSHI, Kazuchika</creatorcontrib><creatorcontrib>RZUCIDLO, S. Jacek</creatorcontrib><creatorcontrib>PRATT, Scott L</creatorcontrib><creatorcontrib>STICE, Steven L</creatorcontrib><title>Utility of Rapidly Matured Oocytes as Recipients for Production of Cloned Embryos from Somatic Cells in the Pig</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The present study was conducted to examine the utility of rapidly matured oocytes as recipients for production of porcine
embryos reconstituted with adult skin fibroblasts and whether arrest of meiotic resumption of recipient oocytes at the germinal
vesicle (GV) stage by dibutyryl cyclic AMP (dbcAMP) improves in vitro developmental rates after reconstruction. At 24 h of
maturation in the medium, 36.3% of oocytes reached the metaphase II (MII) stage. At 30 h of maturation, the percentage (71.4%)
of MII oocytes did not significantly differ from that (78.0%) at 42 h of maturation. When MII oocytes recovered at 24 h of
maturation were used as recipients, 22/156 (14.1%) cloned embryos developing to the blastocyst stage was significantly ( P < 0.05) higher than those of embryos reconstituted with oocytes collected at 30 h (5/168; 3.0%) and 42 h (13/217; 6.0%) of
maturation. Culture of oocytes in medium containing 1 mM dbcAMP for 20 h maintained 72.9% in the GV stage, whereas only 15.0%
of nontreated oocytes were in the GV stage ( P < 0.05). The effect of dbcAMP was reversible. However, the treatment of recipient oocytes with dbcAMP did not affect the
development of reconstructed embryos when compared with nontreated oocytes. These results indicate that rapidly matured oocytes
are superior in their ability to support development of porcine reconstructed embryos; however, arrest of meiotic resumption
of recipient oocytes at the GV stage by dbcAMP does not improve reconstructed embryo developmental rates.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bucladesine - pharmacology</subject><subject>Cloning, Organism - methods</subject><subject>Ear, External - cytology</subject><subject>Ear, External - physiology</subject><subject>Embryo Transfer</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fibroblasts - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Germinal Center - physiology</subject><subject>Metaphase - physiology</subject><subject>Molecular embryology</subject><subject>Oocytes - physiology</subject><subject>Pregnancy</subject><subject>Swine</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0U1v2yAYB3BUbVrSbF-gh4nLenMGfmwwxyrqy6ROibL1bGHADRM2KWBF_vZjaqacuPz4P28I3VCypkTU3zvrXTDH4DXj63JdV-QKLWldioKXrPmAloQQVgAwWKDrGP8QQiso4RNa0JJC3QhYIv-SrLNpxr7He3m02s34p0xTMBpvvZqTiVhGvDfKHq0ZU8S9D3iXa04qWT_--7dxfsz8fujC7DMIfsC__CCTVXhjnIvYjjgdDN7Z18_oYy9dNF_O7wq9PNz_3jwVz9vHH5u75-JQMp4KzRgTFSVNw3Vnqp4RINDXGkBVjZJcyUZ2BLQiNQVWa8lB6IrUXDPRizzZCt2-5-b1vE0mpnawUeVm5Gj8FFtOBTQloRl-PcOpG4xuj8EOMszt_xVl8O0MZFTS9UGOysaLgyZHUXFxB_t6ONlg2jhI53IstKfTifG2bPOJ4C8y4ISc</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>MIYOSHI, Kazuchika</creator><creator>RZUCIDLO, S. Jacek</creator><creator>PRATT, Scott L</creator><creator>STICE, Steven L</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>Utility of Rapidly Matured Oocytes as Recipients for Production of Cloned Embryos from Somatic Cells in the Pig</title><author>MIYOSHI, Kazuchika ; RZUCIDLO, S. Jacek ; PRATT, Scott L ; STICE, Steven L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-d6669410887dbe4f60303f5d33c48ca7ca8ab03dc051365da739d4057d69f9893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bucladesine - pharmacology</topic><topic>Cloning, Organism - methods</topic><topic>Ear, External - cytology</topic><topic>Ear, External - physiology</topic><topic>Embryo Transfer</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fibroblasts - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Germinal Center - physiology</topic><topic>Metaphase - physiology</topic><topic>Molecular embryology</topic><topic>Oocytes - physiology</topic><topic>Pregnancy</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MIYOSHI, Kazuchika</creatorcontrib><creatorcontrib>RZUCIDLO, S. Jacek</creatorcontrib><creatorcontrib>PRATT, Scott L</creatorcontrib><creatorcontrib>STICE, Steven L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MIYOSHI, Kazuchika</au><au>RZUCIDLO, S. Jacek</au><au>PRATT, Scott L</au><au>STICE, Steven L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utility of Rapidly Matured Oocytes as Recipients for Production of Cloned Embryos from Somatic Cells in the Pig</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>67</volume><issue>2</issue><spage>540</spage><epage>545</epage><pages>540-545</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The present study was conducted to examine the utility of rapidly matured oocytes as recipients for production of porcine
embryos reconstituted with adult skin fibroblasts and whether arrest of meiotic resumption of recipient oocytes at the germinal
vesicle (GV) stage by dibutyryl cyclic AMP (dbcAMP) improves in vitro developmental rates after reconstruction. At 24 h of
maturation in the medium, 36.3% of oocytes reached the metaphase II (MII) stage. At 30 h of maturation, the percentage (71.4%)
of MII oocytes did not significantly differ from that (78.0%) at 42 h of maturation. When MII oocytes recovered at 24 h of
maturation were used as recipients, 22/156 (14.1%) cloned embryos developing to the blastocyst stage was significantly ( P < 0.05) higher than those of embryos reconstituted with oocytes collected at 30 h (5/168; 3.0%) and 42 h (13/217; 6.0%) of
maturation. Culture of oocytes in medium containing 1 mM dbcAMP for 20 h maintained 72.9% in the GV stage, whereas only 15.0%
of nontreated oocytes were in the GV stage ( P < 0.05). The effect of dbcAMP was reversible. However, the treatment of recipient oocytes with dbcAMP did not affect the
development of reconstructed embryos when compared with nontreated oocytes. These results indicate that rapidly matured oocytes
are superior in their ability to support development of porcine reconstructed embryos; however, arrest of meiotic resumption
of recipient oocytes at the GV stage by dbcAMP does not improve reconstructed embryo developmental rates.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12135893</pmid><doi>10.1095/biolreprod67.2.540</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 2002-08, Vol.67 (2), p.540-545 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71938201 |
| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Bucladesine - pharmacology Cloning, Organism - methods Ear, External - cytology Ear, External - physiology Embryo Transfer Embryology: invertebrates and vertebrates. Teratology Female Fibroblasts - physiology Fundamental and applied biological sciences. Psychology Germinal Center - physiology Metaphase - physiology Molecular embryology Oocytes - physiology Pregnancy Swine |
| title | Utility of Rapidly Matured Oocytes as Recipients for Production of Cloned Embryos from Somatic Cells in the Pig |
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