Conformational screening of oligonucleotides by variable-temperature high performance liquid chromatography: Dissecting the duplex-hairpin-coil equilibria of d(CGCGAATTCGCG)

This study probes the potential of variable‐temperature high performance liquid chromatography (VT‐HPLC) as a tool for dissecting and modulating nucleic acid structural transitions, using as a model the duplex–hairpin–coil transitions of d(CGCGAATTCGCG). It is demonstrated that VT‐HPLC, combined wit...

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Veröffentlicht in:	Biopolymers 2004-06, Vol.74 (3), p.221-231
Hauptverfasser:	Braunlin, William H., Giri, Indrajit, Beadling, Les, Breslauer, Kenneth J.
Format:	Artikel
Sprache:	eng
Schlagworte:	chromatographic separation Chromatography, High Pressure Liquid Kinetics Nucleic Acid Conformation nucleic acid structural transitions Oligonucleotides - chemistry Oligonucleotides - metabolism structure modulation duplex-hairpin-coil transitions Temperature Thermodynamics variable-temperature high performance liquid chromatography
Online-Zugang:	Volltext
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