Guidance of mesoderm cell migration in the Xenopus gastrula requires PDGF signaling
In vertebrates, PDGFA and its receptor, PDGFRα, are expressed in the early embryo. Impairing their function causes an array of developmental defects, but the underlying target processes that are directly controlled by these factors are not well known. We show that in the Xenopus gastrula, PDGFA/PDG...
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| Veröffentlicht in: | Development (Cambridge) 2004-06, Vol.131 (11), p.2727-2736 |
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| creator | Nagel, Martina Tahinci, Emilios Symes, Karen Winklbauer, Rudolf |
| description | In vertebrates, PDGFA and its receptor, PDGFRα, are expressed in the early embryo. Impairing their function causes an array of developmental defects, but the underlying target processes that are directly controlled by these factors are not well known. We show that in the Xenopus gastrula, PDGFA/PDGFRα signaling is required for the directional migration of mesodermal cells on the extracellular matrix of the blastocoel roof. Blocking PDGFRα function in the mesoderm does not inhibit migration per se, but results in movement that is randomized and no longer directed towards the animal pole. Likewise, compromising PDGFA function in the blastocoel roof substratum abolishes directionality of movement. Overexpression of wild-type PDGFA, or inhibition of PDGFA both lead to randomized migration, disorientation of polarized mesodermal cells, decreased movement towards the animal pole, and reduced head formation and axis elongation. This is consistent with an instructive role for PDGFA in the guidance of mesoderm migration. |
| doi_str_mv | 10.1242/dev.01141 |
| format | Article |
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This is consistent with an instructive role for PDGFA in the guidance of mesoderm migration.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.01141</identifier><identifier>PMID: 15128658</identifier><language>eng</language><publisher>England: The Company of Biologists Limited</publisher><subject>Animals ; Cell Movement - physiology ; Embryo, Nonmammalian ; Embryonic Induction ; Gastrula - cytology ; Gastrula - metabolism ; Gene Expression Regulation, Developmental ; Mesoderm - cytology ; Mesoderm - metabolism ; Platelet-Derived Growth Factor - metabolism ; Receptor, Platelet-Derived Growth Factor alpha - genetics ; Receptor, Platelet-Derived Growth Factor alpha - metabolism ; Signal Transduction ; Xenopus ; Xenopus laevis - embryology ; Xenopus laevis - metabolism ; Xenopus Proteins - genetics ; Xenopus Proteins - metabolism</subject><ispartof>Development (Cambridge), 2004-06, Vol.131 (11), p.2727-2736</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-56d6b0e373b0df0f367b5533e48f8aef92381f2433dfd56d0c86c956ba799b013</citedby><cites>FETCH-LOGICAL-c351t-56d6b0e373b0df0f367b5533e48f8aef92381f2433dfd56d0c86c956ba799b013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3669,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15128658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagel, Martina</creatorcontrib><creatorcontrib>Tahinci, Emilios</creatorcontrib><creatorcontrib>Symes, Karen</creatorcontrib><creatorcontrib>Winklbauer, Rudolf</creatorcontrib><title>Guidance of mesoderm cell migration in the Xenopus gastrula requires PDGF signaling</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>In vertebrates, PDGFA and its receptor, PDGFRα, are expressed in the early embryo. Impairing their function causes an array of developmental defects, but the underlying target processes that are directly controlled by these factors are not well known. We show that in the Xenopus gastrula, PDGFA/PDGFRα signaling is required for the directional migration of mesodermal cells on the extracellular matrix of the blastocoel roof. Blocking PDGFRα function in the mesoderm does not inhibit migration per se, but results in movement that is randomized and no longer directed towards the animal pole. Likewise, compromising PDGFA function in the blastocoel roof substratum abolishes directionality of movement. Overexpression of wild-type PDGFA, or inhibition of PDGFA both lead to randomized migration, disorientation of polarized mesodermal cells, decreased movement towards the animal pole, and reduced head formation and axis elongation. This is consistent with an instructive role for PDGFA in the guidance of mesoderm migration.</description><subject>Animals</subject><subject>Cell Movement - physiology</subject><subject>Embryo, Nonmammalian</subject><subject>Embryonic Induction</subject><subject>Gastrula - cytology</subject><subject>Gastrula - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Mesoderm - cytology</subject><subject>Mesoderm - metabolism</subject><subject>Platelet-Derived Growth Factor - metabolism</subject><subject>Receptor, Platelet-Derived Growth Factor alpha - genetics</subject><subject>Receptor, Platelet-Derived Growth Factor alpha - metabolism</subject><subject>Signal Transduction</subject><subject>Xenopus</subject><subject>Xenopus laevis - embryology</subject><subject>Xenopus laevis - metabolism</subject><subject>Xenopus Proteins - genetics</subject><subject>Xenopus Proteins - metabolism</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFr2zAUx3ExNpY066H_QNFpsIMzPcmyrGNpl7RQ6GAb7CZk-8lRsa1Uslv2389pAj329C4ffjy-hFwAWwPP-fcGn9cMIIcPZAm5UpkGrj-SJdOSZaA1LMhZSo-MMVEo9ZksQAIvC1kuya_t5Bs71EiDoz2m0GDsaY1dR3vfRjv6MFA_0HGH9C8OYT8l2to0xqmzNOLT5CMm-vNmu6HJt4Pt_NB-IZ-c7RKen-6K_Nn8-H19m90_bO-ur-6zWkgYM1k0RcVQKFGxxjE3_1ZJKQTmpSstOs1FCY7nQjSumTGry6LWsqis0rpiIFbk63F3H8PThGk0vU-H1-2AYUpGgRa8YOW7EJQuuSz4DL8dYR1DShGd2Uff2_jPADOH1GZObV5Tz_byNDpVPTZv8tR2Busj2Pl29zJ3MpUPXWh9GtNhB7uwNyDAABiuuBL_AeJKiUU</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Nagel, Martina</creator><creator>Tahinci, Emilios</creator><creator>Symes, Karen</creator><creator>Winklbauer, Rudolf</creator><general>The Company of Biologists Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040601</creationdate><title>Guidance of mesoderm cell migration in the Xenopus gastrula requires PDGF signaling</title><author>Nagel, Martina ; Tahinci, Emilios ; Symes, Karen ; Winklbauer, Rudolf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-56d6b0e373b0df0f367b5533e48f8aef92381f2433dfd56d0c86c956ba799b013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Cell Movement - physiology</topic><topic>Embryo, Nonmammalian</topic><topic>Embryonic Induction</topic><topic>Gastrula - cytology</topic><topic>Gastrula - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Mesoderm - cytology</topic><topic>Mesoderm - metabolism</topic><topic>Platelet-Derived Growth Factor - metabolism</topic><topic>Receptor, Platelet-Derived Growth Factor alpha - genetics</topic><topic>Receptor, Platelet-Derived Growth Factor alpha - metabolism</topic><topic>Signal Transduction</topic><topic>Xenopus</topic><topic>Xenopus laevis - embryology</topic><topic>Xenopus laevis - metabolism</topic><topic>Xenopus Proteins - genetics</topic><topic>Xenopus Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagel, Martina</creatorcontrib><creatorcontrib>Tahinci, Emilios</creatorcontrib><creatorcontrib>Symes, Karen</creatorcontrib><creatorcontrib>Winklbauer, Rudolf</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagel, Martina</au><au>Tahinci, Emilios</au><au>Symes, Karen</au><au>Winklbauer, Rudolf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guidance of mesoderm cell migration in the Xenopus gastrula requires PDGF signaling</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>131</volume><issue>11</issue><spage>2727</spage><epage>2736</epage><pages>2727-2736</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>In vertebrates, PDGFA and its receptor, PDGFRα, are expressed in the early embryo. 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This is consistent with an instructive role for PDGFA in the guidance of mesoderm migration.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>15128658</pmid><doi>10.1242/dev.01141</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 2004-06, Vol.131 (11), p.2727-2736 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Cell Movement - physiology Embryo, Nonmammalian Embryonic Induction Gastrula - cytology Gastrula - metabolism Gene Expression Regulation, Developmental Mesoderm - cytology Mesoderm - metabolism Platelet-Derived Growth Factor - metabolism Receptor, Platelet-Derived Growth Factor alpha - genetics Receptor, Platelet-Derived Growth Factor alpha - metabolism Signal Transduction Xenopus Xenopus laevis - embryology Xenopus laevis - metabolism Xenopus Proteins - genetics Xenopus Proteins - metabolism |
| title | Guidance of mesoderm cell migration in the Xenopus gastrula requires PDGF signaling |
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