[33] Ultrastructural localization of light-induced lipid peroxides
For analysis of transport and processing of lipid peroxides, it is necessary to be able to localize lipid peroxides at the ultrastructural level. A method that uses benzidine-reactive substances and that is able to detect lipid peroxides at the ultrastructural level in rat and mouse tissues is discu...
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| Veröffentlicht in: | Methods in Enzymology 2002, Vol.352, p.378-391 |
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| container_title | Methods in Enzymology |
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| creator | Kayatz, Peter Thumann, Gabriele Schraermeyer, Ulrich |
| description | For analysis of transport and processing of lipid peroxides, it is necessary to be able to localize lipid peroxides at the ultrastructural level. A method that uses benzidine-reactive substances and that is able to detect lipid peroxides at the ultrastructural level in rat and mouse tissues is discussed. The method presented in the chapter offers a simple application for the ultrastructural localization of lipid peroxides that can be used to investigate the formation, decomposition, or transport of lipid peroxides. A method for the ultrastructural localization of lipid peroxides in the retina of rats and mice was developed. The availability of a method for the localization of lipid peroxides at ultrastructural levels, coupled with the effect on the retina of modifications in light exposure, offers the possibility of investigating the formation, transport, and processing of benzidine-reactive substances (BRS) during the digestion of peroxidative-damaged shed tips of rod outer segment (ROS) in the retinal pigment epithelium (RPE). The chapter discusses peroxidation of rat retinas by light exposure using three-month-old long Evans rats, which are kept in single cages under a 12-hr light/dark regimen. The procedures described here are a modification of methods developed for the localization of peroxides in pig and hamster retinas. |
| doi_str_mv | 10.1016/S0076-6879(02)52035-8 |
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The chapter discusses peroxidation of rat retinas by light exposure using three-month-old long Evans rats, which are kept in single cages under a 12-hr light/dark regimen. 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The procedures described here are a modification of methods developed for the localization of peroxides in pig and hamster retinas.</description><subject>Animals</subject><subject>Benzidines - pharmacology</subject><subject>Choroid - metabolism</subject><subject>Electrons</subject><subject>Light</subject><subject>Lipid Peroxidation</subject><subject>Lipid Peroxides - metabolism</subject><subject>Microscopy, Electron - methods</subject><subject>Rats</subject><subject>Rats, Long-Evans</subject><subject>Rod Cell Outer Segment - metabolism</subject><subject>Time Factors</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>9780121822552</isbn><isbn>0121822559</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kFtLAzEQhYMXbNH-BGWfRB9WJ0mzyT6JFm9Q8EH7JBLSZFYj225NdkX99aYXHQaGgW-Gcw4hhxTOKNDi_BFAFnmhZHkC7FQw4CJXW6RPhZC5LJXaJoNSKqCMKsaEYDuk_3_SI4MY3yEV40OuYI_0EscEL0SfXD1z_pJN6jaY2IbOtl0wdVY31tT-x7S-mWdNldX-9a3N_dx1Fl3aFt5lCwzNl3cYD8huZeqIg83cJ5Ob66fRXT5-uL0fXY5zZAVvcw6cFmiFMwYYWldOKZOlpRV3hvJKopQAWAynBR3KJM8wm9paBbICNeR8nxyv_y5C89FhbPXMR4t1bebYdFFLWnKgSibwaAN20xk6vQh-ZsK3_nOdgIs1gEnup8ego_U4T958QNtq13hNQS-j16vo9TJHDUyvoteK_wIUWXGh</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Kayatz, Peter</creator><creator>Thumann, Gabriele</creator><creator>Schraermeyer, Ulrich</creator><general>Elsevier Science & Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>[33] Ultrastructural localization of light-induced lipid peroxides</title><author>Kayatz, Peter ; Thumann, Gabriele ; Schraermeyer, Ulrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e263t-30316ec5daa02ecd9b1279c1f3da13f7e7700e64b6147125a2ca2ccc807f08433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Benzidines - pharmacology</topic><topic>Choroid - metabolism</topic><topic>Electrons</topic><topic>Light</topic><topic>Lipid Peroxidation</topic><topic>Lipid Peroxides - metabolism</topic><topic>Microscopy, Electron - methods</topic><topic>Rats</topic><topic>Rats, Long-Evans</topic><topic>Rod Cell Outer Segment - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kayatz, Peter</creatorcontrib><creatorcontrib>Thumann, Gabriele</creatorcontrib><creatorcontrib>Schraermeyer, Ulrich</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kayatz, Peter</au><au>Thumann, Gabriele</au><au>Schraermeyer, Ulrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[33] Ultrastructural localization of light-induced lipid peroxides</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>2002</date><risdate>2002</risdate><volume>352</volume><spage>378</spage><epage>391</epage><pages>378-391</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>9780121822552</isbn><isbn>0121822559</isbn><abstract>For analysis of transport and processing of lipid peroxides, it is necessary to be able to localize lipid peroxides at the ultrastructural level. 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| ispartof | Methods in Enzymology, 2002, Vol.352, p.378-391 |
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| source | ScienceDirect eBooks; MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Benzidines - pharmacology Choroid - metabolism Electrons Light Lipid Peroxidation Lipid Peroxides - metabolism Microscopy, Electron - methods Rats Rats, Long-Evans Rod Cell Outer Segment - metabolism Time Factors |
| title | [33] Ultrastructural localization of light-induced lipid peroxides |
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