Organization of telomeric nucleosomes: atomic force microscopy imaging and theoretical modeling
Telomeric chromatin has peculiar features with respect to bulk chromatin, which are not fully clarified to date. Nucleosomal arrays, reconstituted on fragments of human telomeric DNA and on tandemly repeated tetramers of 5S rDNA, have been investigated at single-molecule level by atomic force micros...
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| Veröffentlicht in: | FEBS letters 2004-05, Vol.566 (1), p.131-135 |
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| creator | Mechelli, Rosella Anselmi, Claudio Cacchione, Stefano De Santis, Pasquale Savino, Maria |
| description | Telomeric chromatin has peculiar features with respect to bulk chromatin, which are not fully clarified to date. Nucleosomal arrays, reconstituted on fragments of human telomeric DNA and on tandemly repeated tetramers of 5S rDNA, have been investigated at single-molecule level by atomic force microscopy and Monte Carlo simulations. A satisfactory correlation emerges between experimental and theoretical internucleosomal distance distributions. However, in the case of telomeric nucleosomal arrays containing two nucleosomes, we found significant differences. Our results show that sequence features of DNA are significant in the basic chromatin organization, but are not the only determinant. |
| doi_str_mv | 10.1016/j.febslet.2004.04.032 |
| format | Article |
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Nucleosomal arrays, reconstituted on fragments of human telomeric DNA and on tandemly repeated tetramers of 5S rDNA, have been investigated at single-molecule level by atomic force microscopy and Monte Carlo simulations. A satisfactory correlation emerges between experimental and theoretical internucleosomal distance distributions. However, in the case of telomeric nucleosomal arrays containing two nucleosomes, we found significant differences. 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Nucleosomal arrays, reconstituted on fragments of human telomeric DNA and on tandemly repeated tetramers of 5S rDNA, have been investigated at single-molecule level by atomic force microscopy and Monte Carlo simulations. A satisfactory correlation emerges between experimental and theoretical internucleosomal distance distributions. However, in the case of telomeric nucleosomal arrays containing two nucleosomes, we found significant differences. Our results show that sequence features of DNA are significant in the basic chromatin organization, but are not the only determinant.</description><subject>AFM, atomic force microscopy</subject><subject>Animals</subject><subject>Atomic force microscopy</subject><subject>bp, base pair</subject><subject>Chickens</subject><subject>Histones - chemistry</subject><subject>Humans</subject><subject>Microscopy, Atomic Force</subject><subject>Models, Theoretical</subject><subject>Nucleosomal array</subject><subject>Nucleosome positioning</subject><subject>Nucleosomes - chemistry</subject><subject>Nucleosomes - genetics</subject><subject>Nucleosomes - ultrastructure</subject><subject>Reference Values</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA, Ribosomal, 5S - chemistry</subject><subject>RNA, Ribosomal, 5S - genetics</subject><subject>Sea Urchins - genetics</subject><subject>SPD-mica, spermidine-treated mica</subject><subject>Statistical Distributions</subject><subject>Telomere</subject><subject>Telomere - chemistry</subject><subject>Theoretical modeling</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1vFDEMjRAVXVp-AignbrPkcybhgqBqaaVKPbT3KJNxlqwykyWZLVp-PRl2JY5FsmQ7fn6x_RB6T8maEtp-2q499CXCvGaEiPVinL1CK6o63nDRqtdoRQgVjew0P0dvS9mSmiuq36BzKqnolGIrZB7yxk7ht51DmnDyeIaYRsjB4WnvIqRSs_IZ2zmN9c2n7ADXKKfi0u6Aw2g3YdpgOw14_gEpwxycjXhMA8RauERn3sYC707-Aj3dXD9d3Tb3D9_vrr7eN04yLhstrAQ5eK2Bed3TnvReORCUaKal6DrGBAEKggwMPKFWtJL3vm2VVtoO_AJ9PNLucvq5hzKbMRQHMdoJ0r6YjmqmlOheBFLdUS6prEB5BC6rlgze7HJdNh8MJWZRwGzNSQGzKGAW46z2fTh9sO9HGP51nU5eAbdHwK8Q4fB_rObm-ht7XORc1CSiVrq_M345UkG97HOAbIoLMDkYQgY3myGFF6b9AwwYsH8</recordid><startdate>20040521</startdate><enddate>20040521</enddate><creator>Mechelli, Rosella</creator><creator>Anselmi, Claudio</creator><creator>Cacchione, Stefano</creator><creator>De Santis, Pasquale</creator><creator>Savino, Maria</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040521</creationdate><title>Organization of telomeric nucleosomes: atomic force microscopy imaging and theoretical modeling</title><author>Mechelli, Rosella ; 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| subjects | AFM, atomic force microscopy Animals Atomic force microscopy bp, base pair Chickens Histones - chemistry Humans Microscopy, Atomic Force Models, Theoretical Nucleosomal array Nucleosome positioning Nucleosomes - chemistry Nucleosomes - genetics Nucleosomes - ultrastructure Reference Values Repetitive Sequences, Nucleic Acid RNA, Ribosomal, 5S - chemistry RNA, Ribosomal, 5S - genetics Sea Urchins - genetics SPD-mica, spermidine-treated mica Statistical Distributions Telomere Telomere - chemistry Theoretical modeling |
| title | Organization of telomeric nucleosomes: atomic force microscopy imaging and theoretical modeling |
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