A Model System Using Tape Stripping for Characterization of Langerhans Cell-Precursors In Vivo
Little is known about the immigration of bone marrow-derived progenitors of Langerhans cells (LC) into the epidermis. We developed an in vivo system based on the tape stripping method that allowed us to study the immigration of LC into the epidermis after intradermal injection of bone marrow-derived...
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| Veröffentlicht in: | Journal of investigative dermatology 2004-05, Vol.122 (5), p.1165-1174 |
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| container_title | Journal of investigative dermatology |
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| creator | Holzmann, Sandra Tripp, Christoph H. Schmuth, Matthias Janke, Katrin Koch, Franz Saeland, Sem Stoitzner, Patrizia Romani, Nikolaus |
| description | Little is known about the immigration of bone marrow-derived progenitors of Langerhans cells (LC) into the epidermis. We developed an in vivo system based on the tape stripping method that allowed us to study the immigration of LC into the epidermis after intradermal injection of bone marrow-derived dendritic cells (DC). Tape stripping induced a mechanical disruption of the epidermal barrier that led to skin inflammation and subsequent emigration of LC and dermal DC from the skin. Emigrating LC and dermal DC were observed in lymphatic vessels, and the numbers of LC and dermal DC in the draining lymph node increased. Up to 500 times more injected precursors migrated into tape-stripped epidermis as compared with unstripped epidermis. Newly immigrated cells were slender with one or two dendrites and acquired a more dendritic morphology after 2–4 days. They were both MHC II-positive and negative and they did not express Langerin/CD207, nor macrophage-mannose receptor/CD206 and Fc-epsilon receptor I. In contrast, all cells that had entered the epidermis expressed CD11c and CCR6, suggesting that they were LC. We conclude that this experimental system may serve as a valuable tool for the further characterization of LC-precursors and the conditions necessary for LC-immigration into the epidermis. |
| doi_str_mv | 10.1111/j.0022-202X.2004.22520.x |
| format | Article |
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We developed an in vivo system based on the tape stripping method that allowed us to study the immigration of LC into the epidermis after intradermal injection of bone marrow-derived dendritic cells (DC). Tape stripping induced a mechanical disruption of the epidermal barrier that led to skin inflammation and subsequent emigration of LC and dermal DC from the skin. Emigrating LC and dermal DC were observed in lymphatic vessels, and the numbers of LC and dermal DC in the draining lymph node increased. Up to 500 times more injected precursors migrated into tape-stripped epidermis as compared with unstripped epidermis. Newly immigrated cells were slender with one or two dendrites and acquired a more dendritic morphology after 2–4 days. They were both MHC II-positive and negative and they did not express Langerin/CD207, nor macrophage-mannose receptor/CD206 and Fc-epsilon receptor I. In contrast, all cells that had entered the epidermis expressed CD11c and CCR6, suggesting that they were LC. We conclude that this experimental system may serve as a valuable tool for the further characterization of LC-precursors and the conditions necessary for LC-immigration into the epidermis.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/j.0022-202X.2004.22520.x</identifier><identifier>PMID: 15140219</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Animals ; Antigens, Surface - analysis ; Biological and medical sciences ; CD11c Antigen - analysis ; Cell Movement - immunology ; Cells, Cultured ; dendritic cells ; Dermatitis - immunology ; Dermatitis - pathology ; Dermatology ; Dermis - immunology ; Dermis - pathology ; Disease Models, Animal ; epidermis ; Epidermis - immunology ; Epidermis - injuries ; Epidermis - pathology ; Female ; Immunophenotyping ; inflammation ; Langerhans Cells - pathology ; Lectins, C-Type - analysis ; Lymph Nodes - immunology ; Lymphatic System - immunology ; Male ; Mannose-Binding Lectins - analysis ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; migration ; Receptors, CCR6 ; Receptors, Chemokine - analysis ; Stem Cells - chemistry ; Stem Cells - immunology ; Stem Cells - pathology</subject><ispartof>Journal of investigative dermatology, 2004-05, Vol.122 (5), p.1165-1174</ispartof><rights>2004 The Society for Investigative Dermatology, Inc</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Nature Publishing Group May 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-d3dbb750d6f25ac439a8be9af378d35496ebd76b88bcf43a08344dc800063dd03</citedby><cites>FETCH-LOGICAL-c477t-d3dbb750d6f25ac439a8be9af378d35496ebd76b88bcf43a08344dc800063dd03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/210374929?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,64366,64368,64370,72220</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16020877$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15140219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holzmann, Sandra</creatorcontrib><creatorcontrib>Tripp, Christoph H.</creatorcontrib><creatorcontrib>Schmuth, Matthias</creatorcontrib><creatorcontrib>Janke, Katrin</creatorcontrib><creatorcontrib>Koch, Franz</creatorcontrib><creatorcontrib>Saeland, Sem</creatorcontrib><creatorcontrib>Stoitzner, Patrizia</creatorcontrib><creatorcontrib>Romani, Nikolaus</creatorcontrib><title>A Model System Using Tape Stripping for Characterization of Langerhans Cell-Precursors In Vivo</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Little is known about the immigration of bone marrow-derived progenitors of Langerhans cells (LC) into the epidermis. 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In contrast, all cells that had entered the epidermis expressed CD11c and CCR6, suggesting that they were LC. We conclude that this experimental system may serve as a valuable tool for the further characterization of LC-precursors and the conditions necessary for LC-immigration into the epidermis.</description><subject>Animals</subject><subject>Antigens, Surface - analysis</subject><subject>Biological and medical sciences</subject><subject>CD11c Antigen - analysis</subject><subject>Cell Movement - immunology</subject><subject>Cells, Cultured</subject><subject>dendritic cells</subject><subject>Dermatitis - immunology</subject><subject>Dermatitis - pathology</subject><subject>Dermatology</subject><subject>Dermis - immunology</subject><subject>Dermis - pathology</subject><subject>Disease Models, Animal</subject><subject>epidermis</subject><subject>Epidermis - immunology</subject><subject>Epidermis - injuries</subject><subject>Epidermis - pathology</subject><subject>Female</subject><subject>Immunophenotyping</subject><subject>inflammation</subject><subject>Langerhans Cells - pathology</subject><subject>Lectins, C-Type - 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analysis</topic><topic>Biological and medical sciences</topic><topic>CD11c Antigen - analysis</topic><topic>Cell Movement - immunology</topic><topic>Cells, Cultured</topic><topic>dendritic cells</topic><topic>Dermatitis - immunology</topic><topic>Dermatitis - pathology</topic><topic>Dermatology</topic><topic>Dermis - immunology</topic><topic>Dermis - pathology</topic><topic>Disease Models, Animal</topic><topic>epidermis</topic><topic>Epidermis - immunology</topic><topic>Epidermis - injuries</topic><topic>Epidermis - pathology</topic><topic>Female</topic><topic>Immunophenotyping</topic><topic>inflammation</topic><topic>Langerhans Cells - pathology</topic><topic>Lectins, C-Type - analysis</topic><topic>Lymph Nodes - immunology</topic><topic>Lymphatic System - immunology</topic><topic>Male</topic><topic>Mannose-Binding Lectins - analysis</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>migration</topic><topic>Receptors, CCR6</topic><topic>Receptors, Chemokine - 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Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holzmann, Sandra</au><au>Tripp, Christoph H.</au><au>Schmuth, Matthias</au><au>Janke, Katrin</au><au>Koch, Franz</au><au>Saeland, Sem</au><au>Stoitzner, Patrizia</au><au>Romani, Nikolaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Model System Using Tape Stripping for Characterization of Langerhans Cell-Precursors In Vivo</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>122</volume><issue>5</issue><spage>1165</spage><epage>1174</epage><pages>1165-1174</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Little is known about the immigration of bone marrow-derived progenitors of Langerhans cells (LC) into the epidermis. We developed an in vivo system based on the tape stripping method that allowed us to study the immigration of LC into the epidermis after intradermal injection of bone marrow-derived dendritic cells (DC). Tape stripping induced a mechanical disruption of the epidermal barrier that led to skin inflammation and subsequent emigration of LC and dermal DC from the skin. Emigrating LC and dermal DC were observed in lymphatic vessels, and the numbers of LC and dermal DC in the draining lymph node increased. Up to 500 times more injected precursors migrated into tape-stripped epidermis as compared with unstripped epidermis. Newly immigrated cells were slender with one or two dendrites and acquired a more dendritic morphology after 2–4 days. They were both MHC II-positive and negative and they did not express Langerin/CD207, nor macrophage-mannose receptor/CD206 and Fc-epsilon receptor I. In contrast, all cells that had entered the epidermis expressed CD11c and CCR6, suggesting that they were LC. We conclude that this experimental system may serve as a valuable tool for the further characterization of LC-precursors and the conditions necessary for LC-immigration into the epidermis.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>15140219</pmid><doi>10.1111/j.0022-202X.2004.22520.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of investigative dermatology, 2004-05, Vol.122 (5), p.1165-1174 |
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| subjects | Animals Antigens, Surface - analysis Biological and medical sciences CD11c Antigen - analysis Cell Movement - immunology Cells, Cultured dendritic cells Dermatitis - immunology Dermatitis - pathology Dermatology Dermis - immunology Dermis - pathology Disease Models, Animal epidermis Epidermis - immunology Epidermis - injuries Epidermis - pathology Female Immunophenotyping inflammation Langerhans Cells - pathology Lectins, C-Type - analysis Lymph Nodes - immunology Lymphatic System - immunology Male Mannose-Binding Lectins - analysis Medical sciences Mice Mice, Inbred BALB C Mice, Inbred C57BL migration Receptors, CCR6 Receptors, Chemokine - analysis Stem Cells - chemistry Stem Cells - immunology Stem Cells - pathology |
| title | A Model System Using Tape Stripping for Characterization of Langerhans Cell-Precursors In Vivo |
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