Cross-reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro
Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and pr...
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| Veröffentlicht in: | Cell biochemistry and function 2002-09, Vol.20 (3), p.247-256 |
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| creator | Lyons-Alcantara, Maria Lambkin, Helen A. Nordmo, Rolf Lyng, Fiona Mothersill, Carmel |
| description | Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross‐react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p‐glycoprotein were detected by immunocytochemistry in Pandalus borealis. No immunoreactivity for Ki‐67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures. Copyright © 2002 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/cbf.942 |
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This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross‐react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p‐glycoprotein were detected by immunocytochemistry in Pandalus borealis. No immunoreactivity for Ki‐67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures. Copyright © 2002 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/cbf.942</identifier><identifier>PMID: 12125102</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; antibodies ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; cell characterization ; Decapoda (Crustacea) - anatomy & histology ; Decapoda (Crustacea) - immunology ; Digestive System - cytology ; Epithelial Cells - cytology ; Epitopes - chemistry ; Female ; Humans ; Immunohistochemistry ; Keratins - metabolism ; Ki-67 Antigen - biosynthesis ; Marine ; Metallothionein - metabolism ; Oocytes - cytology ; Ovary - cytology ; Pandalus borealis ; Proliferating Cell Nuclear Antigen - metabolism ; ras Proteins - metabolism ; shrimps</subject><ispartof>Cell biochemistry and function, 2002-09, Vol.20 (3), p.247-256</ispartof><rights>Copyright © 2002 John Wiley & Sons, Ltd.</rights><rights>Copyright 2002 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3822-224930321105f2f4adb47926a0d64f41c028dd6d22cb0616721dca6c1712ac4a3</citedby><cites>FETCH-LOGICAL-c3822-224930321105f2f4adb47926a0d64f41c028dd6d22cb0616721dca6c1712ac4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbf.942$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbf.942$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12125102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lyons-Alcantara, Maria</creatorcontrib><creatorcontrib>Lambkin, Helen A.</creatorcontrib><creatorcontrib>Nordmo, Rolf</creatorcontrib><creatorcontrib>Lyng, Fiona</creatorcontrib><creatorcontrib>Mothersill, Carmel</creatorcontrib><title>Cross-reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem. Funct</addtitle><description>Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross‐react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p‐glycoprotein were detected by immunocytochemistry in Pandalus borealis. No immunoreactivity for Ki‐67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures. Copyright © 2002 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>antibodies</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>cell characterization</subject><subject>Decapoda (Crustacea) - anatomy & histology</subject><subject>Decapoda (Crustacea) - immunology</subject><subject>Digestive System - cytology</subject><subject>Epithelial Cells - cytology</subject><subject>Epitopes - chemistry</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keratins - metabolism</subject><subject>Ki-67 Antigen - biosynthesis</subject><subject>Marine</subject><subject>Metallothionein - metabolism</subject><subject>Oocytes - cytology</subject><subject>Ovary - cytology</subject><subject>Pandalus borealis</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>ras Proteins - metabolism</subject><subject>shrimps</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EotOCeAPkFSxQWvvacRJ2MKIt0qh0wd_OcmxHY0jiYDtth2fiIfE0I1ghVpaujs9n3w-hZ5ScUkLgTLfdacPhAVpR0jQFqTl_iFYEBCsEr_kROo7xGyGkEYw8RkcUKJSUwAr9WgcfYxGs0snduLTDvsPRDxarMbnWG2cjTh5v50GN2E4u-SlPbl3a4rgNbpjwtRqN6ueIW581vYt4Cj5ZN8bXWOEp613bZ58z2I34JhNGJefHnGCw3qqQo21wP5dhjtdhjklpmwO17ft4f82l4J-gR53qo316OE_Qp_N3H9eXxebDxfv1m02hWQ1QAPCGEQaUkrKDjivT8qoBoYgRvONUE6iNEQZAt0RQUQE1WglNKwpKc8VO0IvFmz_yY7YxycHF_VPUaP0cZUWbvEDK_wvSumyqqt6DLxdQ79cdbCenvDsVdpISuW9Q5gZlbjCTzw_KuR2s-csdKsvAqwW4db3d_csj12_PF12x0C4me_eHVuG7FBWrSvnl6kLWG_ZZXJdfZc1-A4UOtjY</recordid><startdate>200209</startdate><enddate>200209</enddate><creator>Lyons-Alcantara, Maria</creator><creator>Lambkin, Helen A.</creator><creator>Nordmo, Rolf</creator><creator>Lyng, Fiona</creator><creator>Mothersill, Carmel</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200209</creationdate><title>Cross-reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro</title><author>Lyons-Alcantara, Maria ; 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Funct</addtitle><date>2002-09</date><risdate>2002</risdate><volume>20</volume><issue>3</issue><spage>247</spage><epage>256</epage><pages>247-256</pages><issn>0263-6484</issn><eissn>1099-0844</eissn><abstract>Cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. This fact is hampering advances in the development of tissue cultures from this species. In the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean Pandalus borealis tissues. Several tissues were analysed: hepatopancreas, gills, ovaries, epithelium under the cuticle and abdominal muscle. Specific antibodies to crustacean proteins are not commercially available. The immunocytochemical results show that antibodies to human epitopes cross‐react with antigens in the crustacean Pandalus borealis indicating that some cellular proteins are highly conserved in evolution. Cytokeratin, proliferating cell nuclear antigen, ras and p‐glycoprotein were detected by immunocytochemistry in Pandalus borealis. No immunoreactivity for Ki‐67 and metallothionein was observed. This system can help in validation and characterization of invertebrate cultures. Copyright © 2002 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>12125102</pmid><doi>10.1002/cbf.942</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals antibodies ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism cell characterization Decapoda (Crustacea) - anatomy & histology Decapoda (Crustacea) - immunology Digestive System - cytology Epithelial Cells - cytology Epitopes - chemistry Female Humans Immunohistochemistry Keratins - metabolism Ki-67 Antigen - biosynthesis Marine Metallothionein - metabolism Oocytes - cytology Ovary - cytology Pandalus borealis Proliferating Cell Nuclear Antigen - metabolism ras Proteins - metabolism shrimps |
| title | Cross-reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro |
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