Novel Cyanine Dye-Labeled Dideoxynucleoside Triphosphates for DNA Sequencing

Single color cyanine dye-labeled (Cy 5.0 and Cy 5.5) dideoxynucleoside-5‘-triphosphates, or ‘terminators', containing different spacer lengths were synthesized and evaluated for efficacy in DNA sequencing methods using a modified thermally stable DNA polymerase. The single color cyanine dye ter...

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Veröffentlicht in:	Bioconjugate chemistry 2002-07, Vol.13 (4), p.699-706
Hauptverfasser:	Duthie, R. Scott, Kalve, Inta M, Samols, Sui Bi, Hamilton, Scott, Livshin, Inna, Khot, Mahesh, Nampalli, Satyam, Kumar, Shiv, Fuller, Carl W
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Carbocyanines - chemistry Deoxyribonucleotides - chemistry Dideoxynucleosides - chemistry DNA Probes - chemical synthesis DNA-Directed DNA Polymerase Fluorescent Dyes - chemistry Genetic Carrier Screening Humans Indicators and Reagents Molecular Sequence Data Polymerase Chain Reaction Proteins - analysis Sequence Analysis, DNA - methods
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