Novel Cyanine Dye-Labeled Dideoxynucleoside Triphosphates for DNA Sequencing
Single color cyanine dye-labeled (Cy 5.0 and Cy 5.5) dideoxynucleoside-5‘-triphosphates, or ‘terminators', containing different spacer lengths were synthesized and evaluated for efficacy in DNA sequencing methods using a modified thermally stable DNA polymerase. The single color cyanine dye ter...
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| Veröffentlicht in: | Bioconjugate chemistry 2002-07, Vol.13 (4), p.699-706 |
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| container_issue | 4 |
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| container_title | Bioconjugate chemistry |
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| creator | Duthie, R. Scott Kalve, Inta M Samols, Sui Bi Hamilton, Scott Livshin, Inna Khot, Mahesh Nampalli, Satyam Kumar, Shiv Fuller, Carl W |
| description | Single color cyanine dye-labeled (Cy 5.0 and Cy 5.5) dideoxynucleoside-5‘-triphosphates, or ‘terminators', containing different spacer lengths were synthesized and evaluated for efficacy in DNA sequencing methods using a modified thermally stable DNA polymerase. The single color cyanine dye terminators were formulated into two separate sets of sequencing mixes, one for Cy 5.0 and the other for Cy 5.5, and evaluated on different automated sequencing platforms. Each set of mixes included two pyrimidine terminators with 17-atom linkers and two purine terminators with 10-atom linkers between the dye and the nucleotide. The two sets of cyanine dye-labeled terminators chosen for this cycle sequencing study produced improved band patterns with band uniformity similar to that obtained with dye-primer sequencing methods. |
| doi_str_mv | 10.1021/bc015522y |
| format | Article |
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The two sets of cyanine dye-labeled terminators chosen for this cycle sequencing study produced improved band patterns with band uniformity similar to that obtained with dye-primer sequencing methods.</description><subject>Base Sequence</subject><subject>Carbocyanines - chemistry</subject><subject>Deoxyribonucleotides - chemistry</subject><subject>Dideoxynucleosides - chemistry</subject><subject>DNA Probes - chemical synthesis</subject><subject>DNA-Directed DNA Polymerase</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Genetic Carrier Screening</subject><subject>Humans</subject><subject>Indicators and Reagents</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Proteins - analysis</subject><subject>Sequence Analysis, DNA - methods</subject><issn>1043-1802</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE9LxDAQxYMorv8OfgHpRcFDNZM0m_You7oKi4qu4C2kyVSr3WZNtmK_vZFd9CLvMDPMj_fgEXII9Awog_PSUBCCsX6D7IBgNM1yYJtxpxlPIadsQHZDeKOUFpCzbTIAFgWM75DprfvEJhn1uq1bTMY9plNdYoM2GdcW3VffdqZBF-KRzHy9eHVh8aqXGJLK-WR8e5E84keHranbl32yVekm4MF67pGnq8vZ6Dqd3k1uRhfTVPOsWKZMV9YaEGVmszIzvKRokIMYFoXlJQyr3IpC0sLmMouSecWplhVYLoZAjeF75GTlu_AuZoelmtfBYNPoFl0XlISC5kKyCJ6uQONdCB4rtfD1XPteAVU_1anf6iJ7tDbtyjnaP3LdVQTSFVCHJX79_rV_V0PJpVCz-0c1oQ-SPV_NlIz88YrXJqg31_k2dvJP8De4LoOP</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Duthie, R. 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| ispartof | Bioconjugate chemistry, 2002-07, Vol.13 (4), p.699-706 |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Base Sequence Carbocyanines - chemistry Deoxyribonucleotides - chemistry Dideoxynucleosides - chemistry DNA Probes - chemical synthesis DNA-Directed DNA Polymerase Fluorescent Dyes - chemistry Genetic Carrier Screening Humans Indicators and Reagents Molecular Sequence Data Polymerase Chain Reaction Proteins - analysis Sequence Analysis, DNA - methods |
| title | Novel Cyanine Dye-Labeled Dideoxynucleoside Triphosphates for DNA Sequencing |
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