Characterization of stage-specific and cross-reactive antigens from Eimeria acervulina by chicken monoclonal antibodies
The characterization of five chicken monoclonal antibodies (mAbs) that were developed against apical complex antigens of Eimeria acervulina sporozoites is realized and the mAbs reactivity to merozoites belonging to this species is tested. Using immuno-fluorescence assay (IFA), one mAb (HE-4) that re...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2004, Vol.66(4), pp.403-408 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Constantinoiu, C.C. (Osaka Prefectural Univ., Sakai (Japan)) Lillehoj, H.S Matsubayashi, M Tani, H Matsuda, H Sasai, K Baba, E |
| description | The characterization of five chicken monoclonal antibodies (mAbs) that were developed against apical complex antigens of Eimeria acervulina sporozoites is realized and the mAbs reactivity to merozoites belonging to this species is tested. Using immuno-fluorescence assay (IFA), one mAb (HE-4) that recognized apical antigens common to sporozoites of E. acervulina and E. brunetti bound antigens localized on the apical tip of merozoites from all stages of development examined. The mAb 8E-1, reactive with antigens found on the apical tip of all chicken Eimeria sporozoites, also showed binding to antigens common to merozoites from all generations. Another mAb, 8C-3, which identified an antigen shared by sporozoites apical tip and sporocysts wall of E. acervulina reacted very weak and inconstantly with the merozoites from all generations whereas the mAbs 5D-11 and 8D-2 that recognized antigens shared by the sporozoites of E. acervulina and E. maxima (mAb 5D-11) and E. acervulina and E. brunetti (mAb 8D-2) did not react with the merozoites from any generation. Collectively, these results showed that the invasive stages of chicken Eimeria share cross reactive apical complex antigens which are inter-species and inter-generation-specific that might be components of a potential recombinant vaccine. |
| doi_str_mv | 10.1292/jvms.66.403 |
| format | Article |
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(Osaka Prefectural Univ., Sakai (Japan)) ; Lillehoj, H.S ; Matsubayashi, M ; Tani, H ; Matsuda, H ; Sasai, K ; Baba, E</creator><creatorcontrib>Constantinoiu, C.C. (Osaka Prefectural Univ., Sakai (Japan)) ; Lillehoj, H.S ; Matsubayashi, M ; Tani, H ; Matsuda, H ; Sasai, K ; Baba, E</creatorcontrib><description>The characterization of five chicken monoclonal antibodies (mAbs) that were developed against apical complex antigens of Eimeria acervulina sporozoites is realized and the mAbs reactivity to merozoites belonging to this species is tested. Using immuno-fluorescence assay (IFA), one mAb (HE-4) that recognized apical antigens common to sporozoites of E. acervulina and E. brunetti bound antigens localized on the apical tip of merozoites from all stages of development examined. The mAb 8E-1, reactive with antigens found on the apical tip of all chicken Eimeria sporozoites, also showed binding to antigens common to merozoites from all generations. Another mAb, 8C-3, which identified an antigen shared by sporozoites apical tip and sporocysts wall of E. acervulina reacted very weak and inconstantly with the merozoites from all generations whereas the mAbs 5D-11 and 8D-2 that recognized antigens shared by the sporozoites of E. acervulina and E. maxima (mAb 5D-11) and E. acervulina and E. brunetti (mAb 8D-2) did not react with the merozoites from any generation. Collectively, these results showed that the invasive stages of chicken Eimeria share cross reactive apical complex antigens which are inter-species and inter-generation-specific that might be components of a potential recombinant vaccine.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.66.403</identifier><identifier>PMID: 15133270</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Acervulina ; Animals ; Antibodies, Monoclonal - immunology ; Antigens, Protozoan - immunology ; apical complex ; CHICKENS ; Chickens - immunology ; Chickens - parasitology ; Cross Reactions ; Eimeria ; Eimeria - immunology ; EIMERIA ACERVULINA ; Eimeria brunetti ; Eimeria maxima ; Fluorescent Antibody Technique, Indirect ; IMMUNOFLUORESCENCE ; merozoite ; MONOCLONAL ANTIBODIES ; monoclonal antibody</subject><ispartof>Journal of Veterinary Medical Science, 2004, Vol.66(4), pp.403-408</ispartof><rights>2004 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c707t-37c52a164b23a7d16027f4b22ebae0fe71944a6f7790b1213cbc09d88c4f30f93</citedby><cites>FETCH-LOGICAL-c707t-37c52a164b23a7d16027f4b22ebae0fe71944a6f7790b1213cbc09d88c4f30f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15133270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Constantinoiu, C.C. (Osaka Prefectural Univ., Sakai (Japan))</creatorcontrib><creatorcontrib>Lillehoj, H.S</creatorcontrib><creatorcontrib>Matsubayashi, M</creatorcontrib><creatorcontrib>Tani, H</creatorcontrib><creatorcontrib>Matsuda, H</creatorcontrib><creatorcontrib>Sasai, K</creatorcontrib><creatorcontrib>Baba, E</creatorcontrib><title>Characterization of stage-specific and cross-reactive antigens from Eimeria acervulina by chicken monoclonal antibodies</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>The characterization of five chicken monoclonal antibodies (mAbs) that were developed against apical complex antigens of Eimeria acervulina sporozoites is realized and the mAbs reactivity to merozoites belonging to this species is tested. Using immuno-fluorescence assay (IFA), one mAb (HE-4) that recognized apical antigens common to sporozoites of E. acervulina and E. brunetti bound antigens localized on the apical tip of merozoites from all stages of development examined. The mAb 8E-1, reactive with antigens found on the apical tip of all chicken Eimeria sporozoites, also showed binding to antigens common to merozoites from all generations. Another mAb, 8C-3, which identified an antigen shared by sporozoites apical tip and sporocysts wall of E. acervulina reacted very weak and inconstantly with the merozoites from all generations whereas the mAbs 5D-11 and 8D-2 that recognized antigens shared by the sporozoites of E. acervulina and E. maxima (mAb 5D-11) and E. acervulina and E. brunetti (mAb 8D-2) did not react with the merozoites from any generation. Collectively, these results showed that the invasive stages of chicken Eimeria share cross reactive apical complex antigens which are inter-species and inter-generation-specific that might be components of a potential recombinant vaccine.</description><subject>Acervulina</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Protozoan - immunology</subject><subject>apical complex</subject><subject>CHICKENS</subject><subject>Chickens - immunology</subject><subject>Chickens - parasitology</subject><subject>Cross Reactions</subject><subject>Eimeria</subject><subject>Eimeria - immunology</subject><subject>EIMERIA ACERVULINA</subject><subject>Eimeria brunetti</subject><subject>Eimeria maxima</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>IMMUNOFLUORESCENCE</subject><subject>merozoite</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>monoclonal antibody</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1rFDEYh4Modls9eVYCQi8y65uPTSa3ylK_KOhBzyGTSbZZZyZrMrNS_3qzO0sLXnpJSPK8zwvvLwi9IrAkVNH3232fl0IsObAnaEEYl5XkTD1FC1BEVJKu4Ayd57wFoIQL9RydkRVhjEpYoD_rW5OMHV0Kf80Y4oCjx3k0G1flnbPBB4vN0GKbYs5VcgUNe1euxrBxQ8Y-xR5fh77UG2ysS_upC4PBzR22t8H-cgPu4xBtFwfTHcua2AaXX6Bn3nTZvTztF-jnx-sf68_VzbdPX9YfbiorQY4Vk3ZFDRG8oczIlgig0pcDdY1x4J0kinMjvJQKGkIJs40F1da15Z6BV-wCXc7eXYq_J5dH3YdsXdeZwcUp6yIACfRxkCiqFBfscVDWlArKC_j2P3Abp1TGUBgualHXUhz6vpup44ST83qXQm_SnSagD_nqQ75aCF3yLfSbk3Nqetc-sKdAC3A1A9tjiPeASWOwnXuQzUtx3j_Z8hO0G4ri9azwJmqzSSHrr98pwAoAlCDsH2a3wjs</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Constantinoiu, C.C. (Osaka Prefectural Univ., Sakai (Japan))</creator><creator>Lillehoj, H.S</creator><creator>Matsubayashi, M</creator><creator>Tani, H</creator><creator>Matsuda, H</creator><creator>Sasai, K</creator><creator>Baba, E</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Characterization of stage-specific and cross-reactive antigens from Eimeria acervulina by chicken monoclonal antibodies</title><author>Constantinoiu, C.C. (Osaka Prefectural Univ., Sakai (Japan)) ; Lillehoj, H.S ; Matsubayashi, M ; Tani, H ; Matsuda, H ; Sasai, K ; Baba, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c707t-37c52a164b23a7d16027f4b22ebae0fe71944a6f7790b1213cbc09d88c4f30f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acervulina</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens, Protozoan - immunology</topic><topic>apical complex</topic><topic>CHICKENS</topic><topic>Chickens - immunology</topic><topic>Chickens - parasitology</topic><topic>Cross Reactions</topic><topic>Eimeria</topic><topic>Eimeria - immunology</topic><topic>EIMERIA ACERVULINA</topic><topic>Eimeria brunetti</topic><topic>Eimeria maxima</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>IMMUNOFLUORESCENCE</topic><topic>merozoite</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>monoclonal antibody</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Constantinoiu, C.C. (Osaka Prefectural Univ., Sakai (Japan))</creatorcontrib><creatorcontrib>Lillehoj, H.S</creatorcontrib><creatorcontrib>Matsubayashi, M</creatorcontrib><creatorcontrib>Tani, H</creatorcontrib><creatorcontrib>Matsuda, H</creatorcontrib><creatorcontrib>Sasai, K</creatorcontrib><creatorcontrib>Baba, E</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Constantinoiu, C.C. (Osaka Prefectural Univ., Sakai (Japan))</au><au>Lillehoj, H.S</au><au>Matsubayashi, M</au><au>Tani, H</au><au>Matsuda, H</au><au>Sasai, K</au><au>Baba, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of stage-specific and cross-reactive antigens from Eimeria acervulina by chicken monoclonal antibodies</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>66</volume><issue>4</issue><spage>403</spage><epage>408</epage><pages>403-408</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>The characterization of five chicken monoclonal antibodies (mAbs) that were developed against apical complex antigens of Eimeria acervulina sporozoites is realized and the mAbs reactivity to merozoites belonging to this species is tested. Using immuno-fluorescence assay (IFA), one mAb (HE-4) that recognized apical antigens common to sporozoites of E. acervulina and E. brunetti bound antigens localized on the apical tip of merozoites from all stages of development examined. The mAb 8E-1, reactive with antigens found on the apical tip of all chicken Eimeria sporozoites, also showed binding to antigens common to merozoites from all generations. Another mAb, 8C-3, which identified an antigen shared by sporozoites apical tip and sporocysts wall of E. acervulina reacted very weak and inconstantly with the merozoites from all generations whereas the mAbs 5D-11 and 8D-2 that recognized antigens shared by the sporozoites of E. acervulina and E. maxima (mAb 5D-11) and E. acervulina and E. brunetti (mAb 8D-2) did not react with the merozoites from any generation. Collectively, these results showed that the invasive stages of chicken Eimeria share cross reactive apical complex antigens which are inter-species and inter-generation-specific that might be components of a potential recombinant vaccine.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>15133270</pmid><doi>10.1292/jvms.66.403</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acervulina Animals Antibodies, Monoclonal - immunology Antigens, Protozoan - immunology apical complex CHICKENS Chickens - immunology Chickens - parasitology Cross Reactions Eimeria Eimeria - immunology EIMERIA ACERVULINA Eimeria brunetti Eimeria maxima Fluorescent Antibody Technique, Indirect IMMUNOFLUORESCENCE merozoite MONOCLONAL ANTIBODIES monoclonal antibody |
| title | Characterization of stage-specific and cross-reactive antigens from Eimeria acervulina by chicken monoclonal antibodies |
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