Confocal stereology and image analysis: methods for estimating geometrical characteristics of cells and tissues from three-dimensional confocal images

A short review of confocal stereology and three-dimensional image analysis is presented, pointing out the achievements accomplished in this field by the Department of Biomathematics (Institute of Physiology, Prague). One of the methods of confocal stereology, the fakir method for surface area estima...

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Veröffentlicht in:Physiological research 2004, Vol.53 Suppl 1, p.S47-S55
Hauptverfasser: Kubínová, L, Janácek, J, Karen, P, Radochová, B, Difato, F, Krekule, I
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container_start_page S47
container_title Physiological research
container_volume 53 Suppl 1
creator Kubínová, L
Janácek, J
Karen, P
Radochová, B
Difato, F
Krekule, I
description A short review of confocal stereology and three-dimensional image analysis is presented, pointing out the achievements accomplished in this field by the Department of Biomathematics (Institute of Physiology, Prague). One of the methods of confocal stereology, the fakir method for surface area estimation, developed by this laboratory, is described. Methods for automatic measurement of geometrical characteristics of microscopical structures, based on 3-D image processing or surface triangulation, are discussed and compared with interactive stereological methods. Three-dimensional reconstruction programs and software implementation of stereological and digital methods as well as their practical applications are presented. The future trends are discussed.
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subjects Animals
Chorionic Villi - diagnostic imaging
Humans
Image Processing, Computer-Assisted
Imaging, Three-Dimensional - methods
Mathematics
Microscopy, Confocal - methods
Nicotiana - ultrastructure
Surface Properties
Ultrasonography
title Confocal stereology and image analysis: methods for estimating geometrical characteristics of cells and tissues from three-dimensional confocal images
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