Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain
The minibrain kinase (Mnb/Dyrk1A) gene is localized in the Down syndrome (DS) critical region of chromosome 21. This gene encodes a proline-directed serine/threonine protein kinase (minibrain kinase-Mnb/Dyrk1A), which is required for the proliferation of distinct neuronal cell types during postembry...
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| Veröffentlicht in: | Brain research 2004-06, Vol.1010 (1), p.69-80 |
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| creator | Wegiel, Jerzy Kuchna, Izabela Nowicki, Krzysztof Frackowiak, Janusz Dowjat, Karol Silverman, Wayne P Reisberg, Barry deLeon, Mony Wisniewski, Thomas Adayev, Tatyana Chen-Hwang, Mo-Chou Hwang, Yu-Wen |
| description | The minibrain kinase (Mnb/Dyrk1A) gene is localized in the Down syndrome (DS) critical region of chromosome 21. This gene encodes a proline-directed serine/threonine protein kinase (minibrain kinase-Mnb/Dyrk1A), which is required for the proliferation of distinct neuronal cell types during postembryonic neurogenesis. To study the distribution of Mnb/Dyrk1A during human brain development and aging, we raised Mnb/Dyrk1A-specific antibody (mAb 7F3) and examined 22 brains of normal subjects from 8 months to 90 years of age. We found that neurons were the only cells showing the presence of 7F3-positive product in both cell nucleus and cytoplasm. Nuclear localization supports the concept that Mnb/Dyrk1A may be involved in control of gene expression. Synaptic localization of Mnb/Dyrk1A also supports our previous studies suggesting that Mnb/Dyrk1A is a regulator of assembly of endocytic apparatus and appears to be involved in synaptic vesicle recycling and synaptic signal transmission. Accumulation of numerous 7F3-positive corpora amylacea in the memory and motor system subdivisions in subjects older than 33 years of age indicates that Mnb/Dyrk1A is colocalized with markers of astrocyte and neuron degeneration. Differences in the topography and the amount of Mnb/Dyrk1A in neurons, astrocytes, and ependymal and endothelial cells appear to reflect cell type- and brain structure-specific patterns in trafficking and utilization of Mnb/Dyrk1A. |
| doi_str_mv | 10.1016/j.brainres.2004.03.008 |
| format | Article |
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This gene encodes a proline-directed serine/threonine protein kinase (minibrain kinase-Mnb/Dyrk1A), which is required for the proliferation of distinct neuronal cell types during postembryonic neurogenesis. To study the distribution of Mnb/Dyrk1A during human brain development and aging, we raised Mnb/Dyrk1A-specific antibody (mAb 7F3) and examined 22 brains of normal subjects from 8 months to 90 years of age. We found that neurons were the only cells showing the presence of 7F3-positive product in both cell nucleus and cytoplasm. Nuclear localization supports the concept that Mnb/Dyrk1A may be involved in control of gene expression. Synaptic localization of Mnb/Dyrk1A also supports our previous studies suggesting that Mnb/Dyrk1A is a regulator of assembly of endocytic apparatus and appears to be involved in synaptic vesicle recycling and synaptic signal transmission. Accumulation of numerous 7F3-positive corpora amylacea in the memory and motor system subdivisions in subjects older than 33 years of age indicates that Mnb/Dyrk1A is colocalized with markers of astrocyte and neuron degeneration. Differences in the topography and the amount of Mnb/Dyrk1A in neurons, astrocytes, and ependymal and endothelial cells appear to reflect cell type- and brain structure-specific patterns in trafficking and utilization of Mnb/Dyrk1A.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2004.03.008</identifier><identifier>PMID: 15126119</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Aging - metabolism ; Antibodies ; Astrocytes - cytology ; Astrocytes - enzymology ; Biological and medical sciences ; Biomarkers ; Brain - cytology ; Brain - enzymology ; Brain - growth & development ; Brain aging ; Brain development ; Cell Nucleus - enzymology ; Chromosome aberrations ; Corpora amylacea ; Cytoplasm - enzymology ; Dyrk Kinases ; Endocytosis - physiology ; Endothelial Cells - enzymology ; Ependyma - enzymology ; Female ; Humans ; Immunocytochemistry ; Infant ; Male ; Medical genetics ; Medical sciences ; Middle Aged ; Minibrain kinase ; Nerve Degeneration - enzymology ; Nerve Degeneration - physiopathology ; Neurons - cytology ; Neurons - enzymology ; Presynaptic Terminals - enzymology ; Protein Serine-Threonine Kinases - metabolism ; Protein-Tyrosine Kinases ; Synapse ; Synaptic Transmission - physiology</subject><ispartof>Brain research, 2004-06, Vol.1010 (1), p.69-80</ispartof><rights>2004</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-ad1b6b5b70655ed0ec0957fd16a4bc1b578b4a69b3e9bee18350ff53bf85693</citedby><cites>FETCH-LOGICAL-c491t-ad1b6b5b70655ed0ec0957fd16a4bc1b578b4a69b3e9bee18350ff53bf85693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006899304004007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15718624$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15126119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wegiel, Jerzy</creatorcontrib><creatorcontrib>Kuchna, Izabela</creatorcontrib><creatorcontrib>Nowicki, Krzysztof</creatorcontrib><creatorcontrib>Frackowiak, Janusz</creatorcontrib><creatorcontrib>Dowjat, Karol</creatorcontrib><creatorcontrib>Silverman, Wayne P</creatorcontrib><creatorcontrib>Reisberg, Barry</creatorcontrib><creatorcontrib>deLeon, Mony</creatorcontrib><creatorcontrib>Wisniewski, Thomas</creatorcontrib><creatorcontrib>Adayev, Tatyana</creatorcontrib><creatorcontrib>Chen-Hwang, Mo-Chou</creatorcontrib><creatorcontrib>Hwang, Yu-Wen</creatorcontrib><title>Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The minibrain kinase (Mnb/Dyrk1A) gene is localized in the Down syndrome (DS) critical region of chromosome 21. This gene encodes a proline-directed serine/threonine protein kinase (minibrain kinase-Mnb/Dyrk1A), which is required for the proliferation of distinct neuronal cell types during postembryonic neurogenesis. To study the distribution of Mnb/Dyrk1A during human brain development and aging, we raised Mnb/Dyrk1A-specific antibody (mAb 7F3) and examined 22 brains of normal subjects from 8 months to 90 years of age. We found that neurons were the only cells showing the presence of 7F3-positive product in both cell nucleus and cytoplasm. Nuclear localization supports the concept that Mnb/Dyrk1A may be involved in control of gene expression. Synaptic localization of Mnb/Dyrk1A also supports our previous studies suggesting that Mnb/Dyrk1A is a regulator of assembly of endocytic apparatus and appears to be involved in synaptic vesicle recycling and synaptic signal transmission. Accumulation of numerous 7F3-positive corpora amylacea in the memory and motor system subdivisions in subjects older than 33 years of age indicates that Mnb/Dyrk1A is colocalized with markers of astrocyte and neuron degeneration. Differences in the topography and the amount of Mnb/Dyrk1A in neurons, astrocytes, and ependymal and endothelial cells appear to reflect cell type- and brain structure-specific patterns in trafficking and utilization of Mnb/Dyrk1A.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Aging - metabolism</subject><subject>Antibodies</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - enzymology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Brain - cytology</subject><subject>Brain - enzymology</subject><subject>Brain - growth & development</subject><subject>Brain aging</subject><subject>Brain development</subject><subject>Cell Nucleus - enzymology</subject><subject>Chromosome aberrations</subject><subject>Corpora amylacea</subject><subject>Cytoplasm - enzymology</subject><subject>Dyrk Kinases</subject><subject>Endocytosis - physiology</subject><subject>Endothelial Cells - enzymology</subject><subject>Ependyma - enzymology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunocytochemistry</subject><subject>Infant</subject><subject>Male</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Minibrain kinase</subject><subject>Nerve Degeneration - enzymology</subject><subject>Nerve Degeneration - physiopathology</subject><subject>Neurons - cytology</subject><subject>Neurons - enzymology</subject><subject>Presynaptic Terminals - enzymology</subject><subject>Protein Serine-Threonine Kinases - metabolism</subject><subject>Protein-Tyrosine Kinases</subject><subject>Synapse</subject><subject>Synaptic Transmission - physiology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P1DAMxSMEYoeBr7DqBW4t9qRJmxtotPyRVuIA9yhJXZFhmpYkRdpvT4YOgtueHEu_9-z4MXaL0CCgfHtqbDQ-RErNAaBtgDcA_RO2w7471PLQwlO2AwBZ90rxG_YipVNpOVfwnN2gwINEVDvmjnQ-V_lhoboyYaj-uFYpx9XlNVKdFnJ-9K5aTM4UQ6rmsRp8Abxds5_DpZ988Jvwhw8mUVVe39fJhM3uJXs2mnOiV9e6Z18_3H07fqrvv3z8fHx_X7tWYa7NgFZaYTuQQtAA5ECJbhxQmtY6tKLrbWukspyUJcKeCxhHwe3YC6n4nr3ZXJc4_1wpZT355MrvTKB5TbpDBVxxeBTErkMhiv2eyQ10cU4p0qiX6CcTHzSCvqSgT_pvCvqSggauSwpFeHudsNqJhn-y69kL8PoKmOTMeYwmOJ_-4zrsS4aFe7dxVM72y1PUyXkKjgYfyWU9zP6xXX4DmG2qeA</recordid><startdate>20040604</startdate><enddate>20040604</enddate><creator>Wegiel, Jerzy</creator><creator>Kuchna, Izabela</creator><creator>Nowicki, Krzysztof</creator><creator>Frackowiak, Janusz</creator><creator>Dowjat, Karol</creator><creator>Silverman, Wayne P</creator><creator>Reisberg, Barry</creator><creator>deLeon, Mony</creator><creator>Wisniewski, Thomas</creator><creator>Adayev, Tatyana</creator><creator>Chen-Hwang, Mo-Chou</creator><creator>Hwang, Yu-Wen</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20040604</creationdate><title>Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain</title><author>Wegiel, Jerzy ; Kuchna, Izabela ; Nowicki, Krzysztof ; Frackowiak, Janusz ; Dowjat, Karol ; Silverman, Wayne P ; Reisberg, Barry ; deLeon, Mony ; Wisniewski, Thomas ; Adayev, Tatyana ; Chen-Hwang, Mo-Chou ; Hwang, Yu-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-ad1b6b5b70655ed0ec0957fd16a4bc1b578b4a69b3e9bee18350ff53bf85693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Aging - metabolism</topic><topic>Antibodies</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - enzymology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Brain - cytology</topic><topic>Brain - enzymology</topic><topic>Brain - growth & development</topic><topic>Brain aging</topic><topic>Brain development</topic><topic>Cell Nucleus - enzymology</topic><topic>Chromosome aberrations</topic><topic>Corpora amylacea</topic><topic>Cytoplasm - enzymology</topic><topic>Dyrk Kinases</topic><topic>Endocytosis - physiology</topic><topic>Endothelial Cells - enzymology</topic><topic>Ependyma - enzymology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunocytochemistry</topic><topic>Infant</topic><topic>Male</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Minibrain kinase</topic><topic>Nerve Degeneration - enzymology</topic><topic>Nerve Degeneration - physiopathology</topic><topic>Neurons - cytology</topic><topic>Neurons - enzymology</topic><topic>Presynaptic Terminals - enzymology</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>Protein-Tyrosine Kinases</topic><topic>Synapse</topic><topic>Synaptic Transmission - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wegiel, Jerzy</creatorcontrib><creatorcontrib>Kuchna, Izabela</creatorcontrib><creatorcontrib>Nowicki, Krzysztof</creatorcontrib><creatorcontrib>Frackowiak, Janusz</creatorcontrib><creatorcontrib>Dowjat, Karol</creatorcontrib><creatorcontrib>Silverman, Wayne P</creatorcontrib><creatorcontrib>Reisberg, Barry</creatorcontrib><creatorcontrib>deLeon, Mony</creatorcontrib><creatorcontrib>Wisniewski, Thomas</creatorcontrib><creatorcontrib>Adayev, Tatyana</creatorcontrib><creatorcontrib>Chen-Hwang, Mo-Chou</creatorcontrib><creatorcontrib>Hwang, Yu-Wen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wegiel, Jerzy</au><au>Kuchna, Izabela</au><au>Nowicki, Krzysztof</au><au>Frackowiak, Janusz</au><au>Dowjat, Karol</au><au>Silverman, Wayne P</au><au>Reisberg, Barry</au><au>deLeon, Mony</au><au>Wisniewski, Thomas</au><au>Adayev, Tatyana</au><au>Chen-Hwang, Mo-Chou</au><au>Hwang, Yu-Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2004-06-04</date><risdate>2004</risdate><volume>1010</volume><issue>1</issue><spage>69</spage><epage>80</epage><pages>69-80</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The minibrain kinase (Mnb/Dyrk1A) gene is localized in the Down syndrome (DS) critical region of chromosome 21. This gene encodes a proline-directed serine/threonine protein kinase (minibrain kinase-Mnb/Dyrk1A), which is required for the proliferation of distinct neuronal cell types during postembryonic neurogenesis. To study the distribution of Mnb/Dyrk1A during human brain development and aging, we raised Mnb/Dyrk1A-specific antibody (mAb 7F3) and examined 22 brains of normal subjects from 8 months to 90 years of age. We found that neurons were the only cells showing the presence of 7F3-positive product in both cell nucleus and cytoplasm. Nuclear localization supports the concept that Mnb/Dyrk1A may be involved in control of gene expression. Synaptic localization of Mnb/Dyrk1A also supports our previous studies suggesting that Mnb/Dyrk1A is a regulator of assembly of endocytic apparatus and appears to be involved in synaptic vesicle recycling and synaptic signal transmission. Accumulation of numerous 7F3-positive corpora amylacea in the memory and motor system subdivisions in subjects older than 33 years of age indicates that Mnb/Dyrk1A is colocalized with markers of astrocyte and neuron degeneration. Differences in the topography and the amount of Mnb/Dyrk1A in neurons, astrocytes, and ependymal and endothelial cells appear to reflect cell type- and brain structure-specific patterns in trafficking and utilization of Mnb/Dyrk1A.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>15126119</pmid><doi>10.1016/j.brainres.2004.03.008</doi><tpages>12</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Aging - metabolism Antibodies Astrocytes - cytology Astrocytes - enzymology Biological and medical sciences Biomarkers Brain - cytology Brain - enzymology Brain - growth & development Brain aging Brain development Cell Nucleus - enzymology Chromosome aberrations Corpora amylacea Cytoplasm - enzymology Dyrk Kinases Endocytosis - physiology Endothelial Cells - enzymology Ependyma - enzymology Female Humans Immunocytochemistry Infant Male Medical genetics Medical sciences Middle Aged Minibrain kinase Nerve Degeneration - enzymology Nerve Degeneration - physiopathology Neurons - cytology Neurons - enzymology Presynaptic Terminals - enzymology Protein Serine-Threonine Kinases - metabolism Protein-Tyrosine Kinases Synapse Synaptic Transmission - physiology |
| title | Cell type- and brain structure-specific patterns of distribution of minibrain kinase in human brain |
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