The Catalytic Subunit of Escherichia coli Nitrate Reductase A Contains a Novel [4Fe-4S] Cluster with a High-Spin Ground State

The Catalytic Subunit of Escherichia coli Nitrate Reductase A Contains a Novel [4Fe-4S] Cluster with a High-Spin Ground State

We have used EPR spectroscopy, redox potentiometry, and protein crystallography to characterize the [4Fe-4S] cluster (FS0) of the Escherichia coli nitrate reductase A (NarGHI) catalytic subunit (NarG). FS0 is clearly visible in the crystal structure of NarGHI [Bertero, M. G., et al. (2003) Nat. Stru...

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Veröffentlicht in:Biochemistry (Easton) 2004-05, Vol.43 (18), p.5324-5333
Hauptverfasser: Rothery, Richard A, Bertero, Michela G, Cammack, Richard, Palak, Monica, Blasco, Francis, Strynadka, Natalie C. J, Weiner, Joel H
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Apoproteins - chemistry
Catalytic Domain
Coenzymes - chemistry
Crystallography, X-Ray
Electron Spin Resonance Spectroscopy - methods
Escherichia coli
Escherichia coli Proteins - chemistry
Guanine Nucleotides - chemistry
Iron-Sulfur Proteins - chemistry
Molecular Sequence Data
Molybdenum - chemistry
Nitrate Reductase
Nitrate Reductases - chemistry
Oxidation-Reduction
Potentiometry
Protein Subunits - chemistry
Pterins - chemistry
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Zusammenfassung:We have used EPR spectroscopy, redox potentiometry, and protein crystallography to characterize the [4Fe-4S] cluster (FS0) of the Escherichia coli nitrate reductase A (NarGHI) catalytic subunit (NarG). FS0 is clearly visible in the crystal structure of NarGHI [Bertero, M. G., et al. (2003) Nat. Struct. Biol. 10, 681−687] but has novel coordination comprising one His residue and three Cys residues. At low temperatures (
ISSN:0006-2960
1520-4995
DOI:10.1021/bi049938l