Construction of recombinant pseudorabies virus expressing NS1 protein of Japanese encephalitis (SA14-14-2) virus and its safety and immunogenicity

The bivalent genetic engineering vaccine of Japanese encephalitis (JE) and Aujeszkj disease (AD) was developed to provide a novel approach to prevent and control these two diseases. NS1 gene of Japanese encephalitis virus (JEV) SA14-14-2 strain was produced by reverse transcriptase-mediated PCR (RT-...

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Veröffentlicht in:	Vaccine 2004-05, Vol.22 (15), p.1846-1853
Hauptverfasser:	Xu, Gaoyuan, Xu, Xiaojuan, Li, Zili, He, Qigai, Wu, Bin, Sun, Shengfu, Chen, Huanchun
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Antibodies, Viral - biosynthesis Binding sites Biological and medical sciences Blotting, Western Cell Line Cloning Disease Encephalitis Virus, Japanese - genetics Enzyme-Linked Immunosorbent Assay Enzymes Female Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genomes Hogs Immune response Immunogenicity Infections Japanese Encephalitis Vaccines - adverse effects Japanese Encephalitis Vaccines - genetics Japanese encephalitis virus Laboratories Mice Mice, Inbred BALB C Microbiology Miscellaneous Mortality Plasmids - genetics Protein expression Proteins Pseudorabies - immunology Pseudorabies virus Recombinant virus Reverse Transcriptase Polymerase Chain Reaction Safety Swine T-Lymphocytes, Cytotoxic - immunology Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - adverse effects Vaccines, Synthetic - genetics Vector-borne diseases Viral infections Virology
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