Enzyme-linked Immunosorbent Assay for Detection of Antibodies against Simian Hemorrhagic Fever Virus
Better assays are needed for the detection of simian hemorrhagic fever virus (SHFV), which induces persistent infection without overt signs of disease in most old world monkeys, but causes a fatal hemorrhagic fever in macaques. An enzyme-linked immunosorbent assay (ELISA) is described here that is u...
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| Veröffentlicht in: | Comparative medicine 2002-06, Vol.52 (3), p.229-232 |
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| container_title | Comparative medicine |
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| creator | Godeny, Elmer K. |
| description | Better assays are needed for the detection of simian hemorrhagic fever virus (SHFV), which induces persistent infection without overt signs of disease in most old world monkeys, but causes a fatal hemorrhagic fever in macaques. An enzyme-linked immunosorbent assay (ELISA) is described
here that is useful in identifying primates previously exposed to SHFV. This assay involves testing serum samples against SHFV and cell antigens to obtain an ODvirus-to-ODcell ratio that eliminates potential high background values associated with primate serum. High correlation
was found using this assay, compared with that found with the current "gold standard" indirect immunofluorescence assay (IFA). However, this ELISA is less time consuming, less subjective, and not as prone to human error than the SHFV-IFA. |
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here that is useful in identifying primates previously exposed to SHFV. This assay involves testing serum samples against SHFV and cell antigens to obtain an ODvirus-to-ODcell ratio that eliminates potential high background values associated with primate serum. High correlation
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| ispartof | Comparative medicine, 2002-06, Vol.52 (3), p.229-232 |
| issn | 1532-0820 |
| language | eng |
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| source | MEDLINE; IngentaConnect Free/Open Access Journals; Ingenta Connect; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Arterivirus - immunology Arterivirus - isolation & purification Arterivirus Infections - diagnosis Arterivirus Infections - immunology Arterivirus Infections - veterinary Cercopithecidae Enzyme-Linked Immunosorbent Assay - veterinary Fluorescent Antibody Technique, Indirect Monkey Diseases - immunology Reproducibility of Results |
| title | Enzyme-linked Immunosorbent Assay for Detection of Antibodies against Simian Hemorrhagic Fever Virus |
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