Direct-injection high performance liquid chromatography ion trap mass spectrometry for the quantitative determination of olanzapine, clozapine and N-desmethylclozapine in human plasma
A specific and sensitive direct‐injection high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC/ESI‐MS/MS) method has been developed for the rapid identification and quantitative determination of olanzapine, clozapine, and N‐desmethylclozapine in human plasma....
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| Veröffentlicht in: | Rapid communications in mass spectrometry 2002-01, Vol.16 (13), p.1266-1272 |
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| creator | Kollroser, Manfred Schober, Caroline |
| description | A specific and sensitive direct‐injection high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC/ESI‐MS/MS) method has been developed for the rapid identification and quantitative determination of olanzapine, clozapine, and N‐desmethylclozapine in human plasma. After the addition of the internal standard dibenzepin and dilution with 0.1% formic acid, plasma samples were injected into the LC/MS/MS system. Proteins and other large biomolecules were removed during an online sample cleanup using an extraction column (1 × 50 mm i.d., 30 µm) with a 100% aqueous mobile phase at a flow rate of 4 mL/min. The extraction column was subsequently brought inline with the analytical column by automatic valve switching. Analytes were separated on a 5 µm Symmetry C18 (Waters) analytical column (3.0 × 150 mm) with a mobile phase of acetonitrile/0.1% formic acid (20:80, v/v) at a flow rate of 0.5 mL/min. The total analysis time was 6 min per sample. The inter‐ and intra‐assay coefficients of variation for all compounds were |
| doi_str_mv | 10.1002/rcm.718 |
| format | Article |
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After the addition of the internal standard dibenzepin and dilution with 0.1% formic acid, plasma samples were injected into the LC/MS/MS system. Proteins and other large biomolecules were removed during an online sample cleanup using an extraction column (1 × 50 mm i.d., 30 µm) with a 100% aqueous mobile phase at a flow rate of 4 mL/min. The extraction column was subsequently brought inline with the analytical column by automatic valve switching. Analytes were separated on a 5 µm Symmetry C18 (Waters) analytical column (3.0 × 150 mm) with a mobile phase of acetonitrile/0.1% formic acid (20:80, v/v) at a flow rate of 0.5 mL/min. The total analysis time was 6 min per sample. The inter‐ and intra‐assay coefficients of variation for all compounds were <11%. By eliminating the need for extensive sample preparation, the proposed method offers very large savings in total analysis time. Copyright © 2002 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-4198</identifier><identifier>EISSN: 1097-0231</identifier><identifier>DOI: 10.1002/rcm.718</identifier><identifier>PMID: 12112253</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Antipsychotic Agents - blood ; Benzodiazepines ; Chromatography, High Pressure Liquid - methods ; Clozapine - analogs & derivatives ; Clozapine - blood ; Humans ; Pirenzepine - analogs & derivatives ; Pirenzepine - blood ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrometry, Mass, Electrospray Ionization - methods</subject><ispartof>Rapid communications in mass spectrometry, 2002-01, Vol.16 (13), p.1266-1272</ispartof><rights>Copyright © 2002 John Wiley & Sons, Ltd.</rights><rights>Copyright 2002 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4178-2de2e624e47404719e6789289fbd32c3fb5b53187b7e11c7217a71f79ede2353</citedby><cites>FETCH-LOGICAL-c4178-2de2e624e47404719e6789289fbd32c3fb5b53187b7e11c7217a71f79ede2353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Frcm.718$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Frcm.718$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12112253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kollroser, Manfred</creatorcontrib><creatorcontrib>Schober, Caroline</creatorcontrib><title>Direct-injection high performance liquid chromatography ion trap mass spectrometry for the quantitative determination of olanzapine, clozapine and N-desmethylclozapine in human plasma</title><title>Rapid communications in mass spectrometry</title><addtitle>Rapid Commun. Mass Spectrom</addtitle><description>A specific and sensitive direct‐injection high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC/ESI‐MS/MS) method has been developed for the rapid identification and quantitative determination of olanzapine, clozapine, and N‐desmethylclozapine in human plasma. After the addition of the internal standard dibenzepin and dilution with 0.1% formic acid, plasma samples were injected into the LC/MS/MS system. Proteins and other large biomolecules were removed during an online sample cleanup using an extraction column (1 × 50 mm i.d., 30 µm) with a 100% aqueous mobile phase at a flow rate of 4 mL/min. The extraction column was subsequently brought inline with the analytical column by automatic valve switching. Analytes were separated on a 5 µm Symmetry C18 (Waters) analytical column (3.0 × 150 mm) with a mobile phase of acetonitrile/0.1% formic acid (20:80, v/v) at a flow rate of 0.5 mL/min. The total analysis time was 6 min per sample. The inter‐ and intra‐assay coefficients of variation for all compounds were <11%. By eliminating the need for extensive sample preparation, the proposed method offers very large savings in total analysis time. Copyright © 2002 John Wiley & Sons, Ltd.</description><subject>Antipsychotic Agents - blood</subject><subject>Benzodiazepines</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Clozapine - analogs & derivatives</subject><subject>Clozapine - blood</subject><subject>Humans</subject><subject>Pirenzepine - analogs & derivatives</subject><subject>Pirenzepine - blood</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFv1DAQhS0EoktB_APkExwgxeMk6-SIdqEgyiJVKzhajjNpXJI4aztA-GP8PbxkRU-cZqT53vOTHyFPgV0AY_y10_2FgOIeWQErRcJ4CvfJipU5JBmUxRl55P0tYwA5Zw_JGXAAzvN0RX5vjUMdEjPcxmHsQFtz09IRXWNdrwaNtDOHydRUt872Ktgbp8Z2pkc0xJX2ynvqx6iOdwxuplFJQ4v0MKkhmKCC-Y60xoCuN4P6-4htqO3U8EuNZsBXVHd2WakaarpLavTRqp27u4OJyaYYiI6d8r16TB40qvP45DTPyf7d2_3mfXL1-fLD5s1VojMQRcJr5LjmGWYiY5mAEteiKHlRNlWdcp02VV7lKRSiEgigBQehBDSixChM8_ScPF9sR2cPE_oge-M1djE72snL-Ocly_Iygi8WUDvrvcNGjs70ys0SmDxWJGNFRzySz06WU9VjfcedOonAywX4YTqc_-cjrzefFrtkoY0P-PMfrdw3uRapyOXX3aX88nGXs-vtXm7TP-8Frok</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Kollroser, Manfred</creator><creator>Schober, Caroline</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020101</creationdate><title>Direct-injection high performance liquid chromatography ion trap mass spectrometry for the quantitative determination of olanzapine, clozapine and N-desmethylclozapine in human plasma</title><author>Kollroser, Manfred ; Schober, Caroline</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4178-2de2e624e47404719e6789289fbd32c3fb5b53187b7e11c7217a71f79ede2353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Antipsychotic Agents - blood</topic><topic>Benzodiazepines</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Clozapine - analogs & derivatives</topic><topic>Clozapine - blood</topic><topic>Humans</topic><topic>Pirenzepine - analogs & derivatives</topic><topic>Pirenzepine - blood</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kollroser, Manfred</creatorcontrib><creatorcontrib>Schober, Caroline</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kollroser, Manfred</au><au>Schober, Caroline</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct-injection high performance liquid chromatography ion trap mass spectrometry for the quantitative determination of olanzapine, clozapine and N-desmethylclozapine in human plasma</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><addtitle>Rapid Commun. Mass Spectrom</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>16</volume><issue>13</issue><spage>1266</spage><epage>1272</epage><pages>1266-1272</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>A specific and sensitive direct‐injection high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC/ESI‐MS/MS) method has been developed for the rapid identification and quantitative determination of olanzapine, clozapine, and N‐desmethylclozapine in human plasma. After the addition of the internal standard dibenzepin and dilution with 0.1% formic acid, plasma samples were injected into the LC/MS/MS system. Proteins and other large biomolecules were removed during an online sample cleanup using an extraction column (1 × 50 mm i.d., 30 µm) with a 100% aqueous mobile phase at a flow rate of 4 mL/min. The extraction column was subsequently brought inline with the analytical column by automatic valve switching. 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| ispartof | Rapid communications in mass spectrometry, 2002-01, Vol.16 (13), p.1266-1272 |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Antipsychotic Agents - blood Benzodiazepines Chromatography, High Pressure Liquid - methods Clozapine - analogs & derivatives Clozapine - blood Humans Pirenzepine - analogs & derivatives Pirenzepine - blood Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods |
| title | Direct-injection high performance liquid chromatography ion trap mass spectrometry for the quantitative determination of olanzapine, clozapine and N-desmethylclozapine in human plasma |
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