A comparison between different immobilised glucoseoxidase-based electrodes
Biosensors obtained by immobilising glucose oxidase ‘unentrapped’ and ‘entrapped in liposomes’, both with a classical H 2O 2 amperometric electrode and with screen-printed electrochemical sensor, were compared. Electrode response, linearity range and the influence of some parameters as phospholipid...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2002-08, Vol.29 (6), p.1045-1052 |
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| container_issue | 6 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Memoli, Adriana Annesini, Maria Cristina Mascini, Marco Papale, Stefano Petralito, Stefania |
| description | Biosensors obtained by immobilising glucose oxidase ‘unentrapped’ and ‘entrapped in liposomes’, both with a classical H
2O
2 amperometric electrode and with screen-printed electrochemical sensor, were compared. Electrode response, linearity range and the influence of some parameters as phospholipid nature, temperature and measurement techniques were investigated. Experimental results showed that, while with the unentrapped enzyme the output current is linear only up to about 4 mM glucose concentration, the linearity range increases up to about 20 mM using enzyme-loaded liposomes; however the low permeability of the lipid bilayer decreases the electrode sensitivity to very low values (200 nA/M for palmitoylolelyl phosphatidylcholine liposomes). The approach with screen-printed sensors showed a better performance and gave biosensors with higher sensitivity (about 14 500 nA/mM). A mathematical model, useful to compare the behaviour of the different analytical systems and to design electrodes with the required properties, was also proposed. |
| doi_str_mv | 10.1016/S0731-7085(02)00145-0 |
| format | Article |
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2O
2 amperometric electrode and with screen-printed electrochemical sensor, were compared. Electrode response, linearity range and the influence of some parameters as phospholipid nature, temperature and measurement techniques were investigated. Experimental results showed that, while with the unentrapped enzyme the output current is linear only up to about 4 mM glucose concentration, the linearity range increases up to about 20 mM using enzyme-loaded liposomes; however the low permeability of the lipid bilayer decreases the electrode sensitivity to very low values (200 nA/M for palmitoylolelyl phosphatidylcholine liposomes). The approach with screen-printed sensors showed a better performance and gave biosensors with higher sensitivity (about 14 500 nA/mM). A mathematical model, useful to compare the behaviour of the different analytical systems and to design electrodes with the required properties, was also proposed.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(02)00145-0</identifier><identifier>PMID: 12110389</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Carbohydrates ; Electrochemistry ; Electrodes ; Enzymes, Immobilized - chemistry ; Fundamental and applied biological sciences. Psychology ; Glucose - analysis ; Glucose oxidase ; Glucose Oxidase - chemistry ; Liposome ; Liposomes ; Models, Theoretical ; Oses ; Other biological molecules ; Screen-printed electrodes</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2002-08, Vol.29 (6), p.1045-1052</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-47747dcf4e34b243bacd0721a40bbe89edfc6a2b960c8a64c2fff7f866ae2b623</citedby><cites>FETCH-LOGICAL-c391t-47747dcf4e34b243bacd0721a40bbe89edfc6a2b960c8a64c2fff7f866ae2b623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708502001450$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13794231$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12110389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Memoli, Adriana</creatorcontrib><creatorcontrib>Annesini, Maria Cristina</creatorcontrib><creatorcontrib>Mascini, Marco</creatorcontrib><creatorcontrib>Papale, Stefano</creatorcontrib><creatorcontrib>Petralito, Stefania</creatorcontrib><title>A comparison between different immobilised glucoseoxidase-based electrodes</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>Biosensors obtained by immobilising glucose oxidase ‘unentrapped’ and ‘entrapped in liposomes’, both with a classical H
2O
2 amperometric electrode and with screen-printed electrochemical sensor, were compared. Electrode response, linearity range and the influence of some parameters as phospholipid nature, temperature and measurement techniques were investigated. Experimental results showed that, while with the unentrapped enzyme the output current is linear only up to about 4 mM glucose concentration, the linearity range increases up to about 20 mM using enzyme-loaded liposomes; however the low permeability of the lipid bilayer decreases the electrode sensitivity to very low values (200 nA/M for palmitoylolelyl phosphatidylcholine liposomes). The approach with screen-printed sensors showed a better performance and gave biosensors with higher sensitivity (about 14 500 nA/mM). A mathematical model, useful to compare the behaviour of the different analytical systems and to design electrodes with the required properties, was also proposed.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Carbohydrates</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - analysis</subject><subject>Glucose oxidase</subject><subject>Glucose Oxidase - chemistry</subject><subject>Liposome</subject><subject>Liposomes</subject><subject>Models, Theoretical</subject><subject>Oses</subject><subject>Other biological molecules</subject><subject>Screen-printed electrodes</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1LHDEYwPEgFV3XfgTLXFr0MPrkZSeZk4i0VVnw0BZ6C3l5IpGZyZrM1vrtO-su9egpEH7Pk_An5ITCOQXaXPwAyWktQS1OgZ0BULGoYY_MqJK8Zo34_YHM_pNDclTKIwAsaCsOyCFllAJX7YzcXVUu9SuTY0lDZXF8RhwqH0PAjMNYxb5PNnaxoK8eurVLBdPf6E3B2prNJXboxpw8lmOyH0xX8OPunJNf377-vL6pl_ffb6-vlrXjLR1rIaWQ3gWBXFgmuDXOg2TUCLAWVYs-uMYw2zbglGmEYyEEGVTTGGS2YXxOvmz3rnJ6WmMZdR-Lw64zA6Z10ZIq1XKuJrjYQpdTKRmDXuXYm_yiKehNRP0aUW8KaWD6NaKGae7T7oG17dG_Te2qTeDzDpjiTBeyGVwsb47LVjBOJ3e5dTjl-BMx6-IiDg59zFM17VN85yv_AIjPj5k</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Memoli, Adriana</creator><creator>Annesini, Maria Cristina</creator><creator>Mascini, Marco</creator><creator>Papale, Stefano</creator><creator>Petralito, Stefania</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>A comparison between different immobilised glucoseoxidase-based electrodes</title><author>Memoli, Adriana ; Annesini, Maria Cristina ; Mascini, Marco ; Papale, Stefano ; Petralito, Stefania</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-47747dcf4e34b243bacd0721a40bbe89edfc6a2b960c8a64c2fff7f866ae2b623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Carbohydrates</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - analysis</topic><topic>Glucose oxidase</topic><topic>Glucose Oxidase - chemistry</topic><topic>Liposome</topic><topic>Liposomes</topic><topic>Models, Theoretical</topic><topic>Oses</topic><topic>Other biological molecules</topic><topic>Screen-printed electrodes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Memoli, Adriana</creatorcontrib><creatorcontrib>Annesini, Maria Cristina</creatorcontrib><creatorcontrib>Mascini, Marco</creatorcontrib><creatorcontrib>Papale, Stefano</creatorcontrib><creatorcontrib>Petralito, Stefania</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Memoli, Adriana</au><au>Annesini, Maria Cristina</au><au>Mascini, Marco</au><au>Papale, Stefano</au><au>Petralito, Stefania</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison between different immobilised glucoseoxidase-based electrodes</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>29</volume><issue>6</issue><spage>1045</spage><epage>1052</epage><pages>1045-1052</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>Biosensors obtained by immobilising glucose oxidase ‘unentrapped’ and ‘entrapped in liposomes’, both with a classical H
2O
2 amperometric electrode and with screen-printed electrochemical sensor, were compared. Electrode response, linearity range and the influence of some parameters as phospholipid nature, temperature and measurement techniques were investigated. Experimental results showed that, while with the unentrapped enzyme the output current is linear only up to about 4 mM glucose concentration, the linearity range increases up to about 20 mM using enzyme-loaded liposomes; however the low permeability of the lipid bilayer decreases the electrode sensitivity to very low values (200 nA/M for palmitoylolelyl phosphatidylcholine liposomes). The approach with screen-printed sensors showed a better performance and gave biosensors with higher sensitivity (about 14 500 nA/mM). A mathematical model, useful to compare the behaviour of the different analytical systems and to design electrodes with the required properties, was also proposed.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12110389</pmid><doi>10.1016/S0731-7085(02)00145-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical and biomedical analysis, 2002-08, Vol.29 (6), p.1045-1052 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Biosensing Techniques - methods Biosensors Carbohydrates Electrochemistry Electrodes Enzymes, Immobilized - chemistry Fundamental and applied biological sciences. Psychology Glucose - analysis Glucose oxidase Glucose Oxidase - chemistry Liposome Liposomes Models, Theoretical Oses Other biological molecules Screen-printed electrodes |
| title | A comparison between different immobilised glucoseoxidase-based electrodes |
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