Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor
We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein–protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibod...
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| Veröffentlicht in: | Biomolecular engineering 2004-04, Vol.21 (2), p.67-72 |
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| container_end_page | 72 |
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| container_title | Biomolecular engineering |
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| creator | Zhong, Joan Q. Freyzon, Yelena Ehrlich, Daniel J. Matsudaira, Paul |
| description | We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein–protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface. |
| doi_str_mv | 10.1016/j.bioeng.2003.10.004 |
| format | Article |
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The aFPs detect protein–protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. 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The aFPs detect protein–protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface.</description><subject>Affinity fluorescent protein</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Binding Sites - genetics</subject><subject>Biosensing Techniques</subject><subject>Biosensor</subject><subject>Enhanced fluorescence intensity</subject><subject>Fluorescence</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Hemagglutinins - chemistry</subject><subject>Hemagglutinins - genetics</subject><subject>Hemagglutinins - metabolism</subject><subject>Protein Binding - genetics</subject><subject>Protein Engineering</subject><subject>Protein Structure, Tertiary</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Solid-phase assay</subject><subject>Spectrometry, Fluorescence</subject><subject>Surface Properties</subject><issn>1389-0344</issn><issn>1878-559X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9rHSEUxSW0JGmSbxCKq-7mVcc_42wKJaRpIdBNC9mJo9fExzx9VedBvn0d3oPu0pWXyznHw_0hdEvJhhIqP283U0gQnzc9IaytNoTwM3RJ1aA6Icand21mauwI4_wCfShlSwjlhLNzdEEFpUxIconm-_hiogWHHVSwNaSIC8QSajiE-oqXEuIzNjimA8y4pDm4bv9iCuAQbcr7lE1tZuN9iKvez0vKUCzEivc5VQgRt55rZMrX6L03c4Gb03uFfn-7_3X3vXv8-fDj7utjZ7kQteudpNMojRuU9IKwnoyToQacE6ME4iYLhE29ULxN1nqw0hgvGAy9Et4xdoU-HXNbgz8LlKp3oVWaZxMhLUUPVCklevlfIR0Ul4yqJuRHoc2plAxe73PYmfyqKdErDr3VRxx6xbFuG45m-3jKX6YduH-m0_2b4MtRAO0chwBZFxtg5RFyo6FdCm__8Bdd2qFV</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Zhong, Joan Q.</creator><creator>Freyzon, Yelena</creator><creator>Ehrlich, Daniel J.</creator><creator>Matsudaira, Paul</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor</title><author>Zhong, Joan Q. ; Freyzon, Yelena ; Ehrlich, Daniel J. ; Matsudaira, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-2d61b96ad786f503209ba1aedd596e0dbce03b2584bceccfec6aaf53e7285fd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Affinity fluorescent protein</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>Binding Sites - genetics</topic><topic>Biosensing Techniques</topic><topic>Biosensor</topic><topic>Enhanced fluorescence intensity</topic><topic>Fluorescence</topic><topic>Green fluorescent protein</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Hemagglutinins - chemistry</topic><topic>Hemagglutinins - genetics</topic><topic>Hemagglutinins - metabolism</topic><topic>Protein Binding - genetics</topic><topic>Protein Engineering</topic><topic>Protein Structure, Tertiary</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Solid-phase assay</topic><topic>Spectrometry, Fluorescence</topic><topic>Surface Properties</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhong, Joan Q.</creatorcontrib><creatorcontrib>Freyzon, Yelena</creatorcontrib><creatorcontrib>Ehrlich, Daniel J.</creatorcontrib><creatorcontrib>Matsudaira, Paul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomolecular engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhong, Joan Q.</au><au>Freyzon, Yelena</au><au>Ehrlich, Daniel J.</au><au>Matsudaira, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor</atitle><jtitle>Biomolecular engineering</jtitle><addtitle>Biomol Eng</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>21</volume><issue>2</issue><spage>67</spage><epage>72</epage><pages>67-72</pages><issn>1389-0344</issn><eissn>1878-559X</eissn><abstract>We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein–protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15113560</pmid><doi>10.1016/j.bioeng.2003.10.004</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-0344 |
| ispartof | Biomolecular engineering, 2004-04, Vol.21 (2), p.67-72 |
| issn | 1389-0344 1878-559X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Affinity fluorescent protein Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - metabolism Binding Sites - genetics Biosensing Techniques Biosensor Enhanced fluorescence intensity Fluorescence Green fluorescent protein Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Hemagglutinins - chemistry Hemagglutinins - genetics Hemagglutinins - metabolism Protein Binding - genetics Protein Engineering Protein Structure, Tertiary Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sensitivity and Specificity Solid-phase assay Spectrometry, Fluorescence Surface Properties |
| title | Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor |
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