Screening for novel essential genes of Saccharomyces cerevisiae involved in protein secretion

We describe here a screening procedure devised for searching new genes involved in protein secretion in Saccharomyces cerevisiae. The screening procedure takes advantage of yeast strains constructed within the EUROFAN project, in which the promoters of the novel essential genes were replaced by the...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2004-04, Vol.21 (6), p.463-471
Hauptverfasser:	Davydenko, Svetlana G., Juselius, Jukka K., Munder, Thomas, Bogengruber, Edith, Jäntti, Jussi, Keränen, Sirkka
Format:	Artikel
Sprache:	eng
Schlagworte:	Blotting, Western Doxycycline - pharmacology Genes, Essential - drug effects Genes, Essential - genetics Genes, Fungal - drug effects Genes, Fungal - genetics Glycoproteins - genetics Glycoproteins - metabolism Glycoproteins - secretion Heat-Shock Proteins - genetics Heat-Shock Proteins - metabolism Heat-Shock Proteins - secretion HSP150/PIR2 novel essential genes Open Reading Frames Promoter Regions, Genetic - drug effects Promoter Regions, Genetic - genetics protein secretion ribosome assembly Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Saccharomyces cerevisiae Proteins - secretion
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