Maldi-TOF analysis of portal sera of pancreatic cancer patients: identification of diabetogenic and antidiabetogenic peptides
Background: Pancreatic cancer (PC) associated diabetes mellitus (DM) might be consequent to the diabetogenic effects of tumour products, possibly acting via nitric oxide (NO). Our aims were: (1) to verify whether PC associated DM determines an increased hepatic NO and (2) using MALDI-TOF analysis, t...
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| Veröffentlicht in: | Clinica chimica acta 2004-05, Vol.343 (1), p.119-127 |
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| creator | Valerio, Anna Basso, Daniela Fogar, Paola Falconi, Massimo Greco, Eliana Bassi, Claudio Seraglia, Roberta Abu-Hilal, Mohamed Navaglia, Filippo Zambon, Carlo-Federico Gallo, Nicoletta Falda, Alessandra Pedrazzoli, Sergio Plebani, Mario |
| description | Background: Pancreatic cancer (PC) associated diabetes mellitus (DM) might be consequent to the diabetogenic effects of tumour products, possibly acting via nitric oxide (NO). Our aims were: (1) to verify whether PC associated DM determines an increased hepatic NO and (2) using MALDI-TOF analysis, to evaluate the peptide composition of PC cell conditioned media (CM) and of portal sera from patients with PC with (
n=7) or without (
n=4) DM.
Methods: In liver tissue homogenates of 23 patients with PC (
n=17) or chronic pancreatitis (
n=6) GAPDH mRNA and activity, glucose, lactate, nitrite and nitrate were assayed. MALDI-TOF analysis was performed in three PC cell lines CM, and in portal sera from patients with PC.
Results: Higher GAPDH mRNA and nitrite were found in patients with than in patients without DM. In PC cell CM, only 9 among a total of 75 fragments identified, were tumour specific. One hundred seventy-three fragments were identified in the portal sera of patients: one was positively and six fragments were negatively correlated with DM.
Conclusions: Unlike liver GAPDH, NO appears to be involved in PC associated DM. In portal sera, the absence, rather than the presence, of specific fragments, appears to be correlated with the development of DM. |
| doi_str_mv | 10.1016/j.cccn.2003.12.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71883713</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009898104000075</els_id><sourcerecordid>71883713</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354t-ee107b34a8ca77707f8b0a24d5c899d18040b889cac52f2e1085886745657c003</originalsourceid><addsrcrecordid>eNp9kE1v1DAQhi1ERbctf4ADyolbgsf5sIO4oIpSpFa9tGfLGU-QV9kk2F6kHvrfmWVXQlw4WON59cxI8wjxDmQFErqP2woR50pJWVegKqngldiA0XVZN716LTZSyr40vYFzcZHSlttGdvBGnEML0Ham3oiXezf5UD4-3BRudtNzCqlYxmJdYnZTkSi6P62bMZLLAQvkL0VOcqA5p09F8FzDGJCTZT7QPriB8vKDZubd7Pnl8E-40soJpStxNrop0dtTvRRPN18fr2_Lu4dv36-_3JVYt00uiUDqoW6cQae1lno0g3Sq8S2avvdg-K7BmB4dtmpUTJvWmE43bddqZD2X4sNx7xqXn3tK2e5CQpomN9OyT1aDMbWGmkF1BDEuKUUa7RrDzsVnC9IepNutPUi3B-kWlGXpPPT-tH0_7Mj_HTlZZuDzESC-8VegaBOyPSQfImG2fgn_2_8bYdKUBQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71883713</pqid></control><display><type>article</type><title>Maldi-TOF analysis of portal sera of pancreatic cancer patients: identification of diabetogenic and antidiabetogenic peptides</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Valerio, Anna ; Basso, Daniela ; Fogar, Paola ; Falconi, Massimo ; Greco, Eliana ; Bassi, Claudio ; Seraglia, Roberta ; Abu-Hilal, Mohamed ; Navaglia, Filippo ; Zambon, Carlo-Federico ; Gallo, Nicoletta ; Falda, Alessandra ; Pedrazzoli, Sergio ; Plebani, Mario</creator><creatorcontrib>Valerio, Anna ; Basso, Daniela ; Fogar, Paola ; Falconi, Massimo ; Greco, Eliana ; Bassi, Claudio ; Seraglia, Roberta ; Abu-Hilal, Mohamed ; Navaglia, Filippo ; Zambon, Carlo-Federico ; Gallo, Nicoletta ; Falda, Alessandra ; Pedrazzoli, Sergio ; Plebani, Mario</creatorcontrib><description>Background: Pancreatic cancer (PC) associated diabetes mellitus (DM) might be consequent to the diabetogenic effects of tumour products, possibly acting via nitric oxide (NO). Our aims were: (1) to verify whether PC associated DM determines an increased hepatic NO and (2) using MALDI-TOF analysis, to evaluate the peptide composition of PC cell conditioned media (CM) and of portal sera from patients with PC with (
n=7) or without (
n=4) DM.
Methods: In liver tissue homogenates of 23 patients with PC (
n=17) or chronic pancreatitis (
n=6) GAPDH mRNA and activity, glucose, lactate, nitrite and nitrate were assayed. MALDI-TOF analysis was performed in three PC cell lines CM, and in portal sera from patients with PC.
Results: Higher GAPDH mRNA and nitrite were found in patients with than in patients without DM. In PC cell CM, only 9 among a total of 75 fragments identified, were tumour specific. One hundred seventy-three fragments were identified in the portal sera of patients: one was positively and six fragments were negatively correlated with DM.
Conclusions: Unlike liver GAPDH, NO appears to be involved in PC associated DM. In portal sera, the absence, rather than the presence, of specific fragments, appears to be correlated with the development of DM.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/j.cccn.2003.12.021</identifier><identifier>PMID: 15115683</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aged ; Aged, 80 and over ; Biomarkers - analysis ; Cell Line, Tumor ; Cells, Cultured ; Diabetes Complications - blood ; Diabetes Complications - genetics ; Diabetes Complications - metabolism ; Diabetes mellitus ; Female ; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics ; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism ; Humans ; Liver - enzymology ; Liver - metabolism ; Maldi-TOF ; Male ; Middle Aged ; Nitric oxide ; Nitrites - analysis ; Pancreatic cancer ; Pancreatic Neoplasms - blood ; Pancreatic Neoplasms - complications ; Pancreatic Neoplasms - genetics ; Pancreatic Neoplasms - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>Clinica chimica acta, 2004-05, Vol.343 (1), p.119-127</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-ee107b34a8ca77707f8b0a24d5c899d18040b889cac52f2e1085886745657c003</citedby><cites>FETCH-LOGICAL-c354t-ee107b34a8ca77707f8b0a24d5c899d18040b889cac52f2e1085886745657c003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0009898104000075$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15115683$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valerio, Anna</creatorcontrib><creatorcontrib>Basso, Daniela</creatorcontrib><creatorcontrib>Fogar, Paola</creatorcontrib><creatorcontrib>Falconi, Massimo</creatorcontrib><creatorcontrib>Greco, Eliana</creatorcontrib><creatorcontrib>Bassi, Claudio</creatorcontrib><creatorcontrib>Seraglia, Roberta</creatorcontrib><creatorcontrib>Abu-Hilal, Mohamed</creatorcontrib><creatorcontrib>Navaglia, Filippo</creatorcontrib><creatorcontrib>Zambon, Carlo-Federico</creatorcontrib><creatorcontrib>Gallo, Nicoletta</creatorcontrib><creatorcontrib>Falda, Alessandra</creatorcontrib><creatorcontrib>Pedrazzoli, Sergio</creatorcontrib><creatorcontrib>Plebani, Mario</creatorcontrib><title>Maldi-TOF analysis of portal sera of pancreatic cancer patients: identification of diabetogenic and antidiabetogenic peptides</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Background: Pancreatic cancer (PC) associated diabetes mellitus (DM) might be consequent to the diabetogenic effects of tumour products, possibly acting via nitric oxide (NO). Our aims were: (1) to verify whether PC associated DM determines an increased hepatic NO and (2) using MALDI-TOF analysis, to evaluate the peptide composition of PC cell conditioned media (CM) and of portal sera from patients with PC with (
n=7) or without (
n=4) DM.
Methods: In liver tissue homogenates of 23 patients with PC (
n=17) or chronic pancreatitis (
n=6) GAPDH mRNA and activity, glucose, lactate, nitrite and nitrate were assayed. MALDI-TOF analysis was performed in three PC cell lines CM, and in portal sera from patients with PC.
Results: Higher GAPDH mRNA and nitrite were found in patients with than in patients without DM. In PC cell CM, only 9 among a total of 75 fragments identified, were tumour specific. One hundred seventy-three fragments were identified in the portal sera of patients: one was positively and six fragments were negatively correlated with DM.
Conclusions: Unlike liver GAPDH, NO appears to be involved in PC associated DM. In portal sera, the absence, rather than the presence, of specific fragments, appears to be correlated with the development of DM.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers - analysis</subject><subject>Cell Line, Tumor</subject><subject>Cells, Cultured</subject><subject>Diabetes Complications - blood</subject><subject>Diabetes Complications - genetics</subject><subject>Diabetes Complications - metabolism</subject><subject>Diabetes mellitus</subject><subject>Female</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - genetics</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</subject><subject>Humans</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Maldi-TOF</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nitric oxide</subject><subject>Nitrites - analysis</subject><subject>Pancreatic cancer</subject><subject>Pancreatic Neoplasms - blood</subject><subject>Pancreatic Neoplasms - complications</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi1ERbctf4ADyolbgsf5sIO4oIpSpFa9tGfLGU-QV9kk2F6kHvrfmWVXQlw4WON59cxI8wjxDmQFErqP2woR50pJWVegKqngldiA0XVZN716LTZSyr40vYFzcZHSlttGdvBGnEML0Ham3oiXezf5UD4-3BRudtNzCqlYxmJdYnZTkSi6P62bMZLLAQvkL0VOcqA5p09F8FzDGJCTZT7QPriB8vKDZubd7Pnl8E-40soJpStxNrop0dtTvRRPN18fr2_Lu4dv36-_3JVYt00uiUDqoW6cQae1lno0g3Sq8S2avvdg-K7BmB4dtmpUTJvWmE43bddqZD2X4sNx7xqXn3tK2e5CQpomN9OyT1aDMbWGmkF1BDEuKUUa7RrDzsVnC9IepNutPUi3B-kWlGXpPPT-tH0_7Mj_HTlZZuDzESC-8VegaBOyPSQfImG2fgn_2_8bYdKUBQ</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Valerio, Anna</creator><creator>Basso, Daniela</creator><creator>Fogar, Paola</creator><creator>Falconi, Massimo</creator><creator>Greco, Eliana</creator><creator>Bassi, Claudio</creator><creator>Seraglia, Roberta</creator><creator>Abu-Hilal, Mohamed</creator><creator>Navaglia, Filippo</creator><creator>Zambon, Carlo-Federico</creator><creator>Gallo, Nicoletta</creator><creator>Falda, Alessandra</creator><creator>Pedrazzoli, Sergio</creator><creator>Plebani, Mario</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>Maldi-TOF analysis of portal sera of pancreatic cancer patients: identification of diabetogenic and antidiabetogenic peptides</title><author>Valerio, Anna ; Basso, Daniela ; Fogar, Paola ; Falconi, Massimo ; Greco, Eliana ; Bassi, Claudio ; Seraglia, Roberta ; Abu-Hilal, Mohamed ; Navaglia, Filippo ; Zambon, Carlo-Federico ; Gallo, Nicoletta ; Falda, Alessandra ; Pedrazzoli, Sergio ; Plebani, Mario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-ee107b34a8ca77707f8b0a24d5c899d18040b889cac52f2e1085886745657c003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers - analysis</topic><topic>Cell Line, Tumor</topic><topic>Cells, Cultured</topic><topic>Diabetes Complications - blood</topic><topic>Diabetes Complications - genetics</topic><topic>Diabetes Complications - metabolism</topic><topic>Diabetes mellitus</topic><topic>Female</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - genetics</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</topic><topic>Humans</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Maldi-TOF</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nitric oxide</topic><topic>Nitrites - analysis</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms - blood</topic><topic>Pancreatic Neoplasms - complications</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valerio, Anna</creatorcontrib><creatorcontrib>Basso, Daniela</creatorcontrib><creatorcontrib>Fogar, Paola</creatorcontrib><creatorcontrib>Falconi, Massimo</creatorcontrib><creatorcontrib>Greco, Eliana</creatorcontrib><creatorcontrib>Bassi, Claudio</creatorcontrib><creatorcontrib>Seraglia, Roberta</creatorcontrib><creatorcontrib>Abu-Hilal, Mohamed</creatorcontrib><creatorcontrib>Navaglia, Filippo</creatorcontrib><creatorcontrib>Zambon, Carlo-Federico</creatorcontrib><creatorcontrib>Gallo, Nicoletta</creatorcontrib><creatorcontrib>Falda, Alessandra</creatorcontrib><creatorcontrib>Pedrazzoli, Sergio</creatorcontrib><creatorcontrib>Plebani, Mario</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valerio, Anna</au><au>Basso, Daniela</au><au>Fogar, Paola</au><au>Falconi, Massimo</au><au>Greco, Eliana</au><au>Bassi, Claudio</au><au>Seraglia, Roberta</au><au>Abu-Hilal, Mohamed</au><au>Navaglia, Filippo</au><au>Zambon, Carlo-Federico</au><au>Gallo, Nicoletta</au><au>Falda, Alessandra</au><au>Pedrazzoli, Sergio</au><au>Plebani, Mario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maldi-TOF analysis of portal sera of pancreatic cancer patients: identification of diabetogenic and antidiabetogenic peptides</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>343</volume><issue>1</issue><spage>119</spage><epage>127</epage><pages>119-127</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Background: Pancreatic cancer (PC) associated diabetes mellitus (DM) might be consequent to the diabetogenic effects of tumour products, possibly acting via nitric oxide (NO). Our aims were: (1) to verify whether PC associated DM determines an increased hepatic NO and (2) using MALDI-TOF analysis, to evaluate the peptide composition of PC cell conditioned media (CM) and of portal sera from patients with PC with (
n=7) or without (
n=4) DM.
Methods: In liver tissue homogenates of 23 patients with PC (
n=17) or chronic pancreatitis (
n=6) GAPDH mRNA and activity, glucose, lactate, nitrite and nitrate were assayed. MALDI-TOF analysis was performed in three PC cell lines CM, and in portal sera from patients with PC.
Results: Higher GAPDH mRNA and nitrite were found in patients with than in patients without DM. In PC cell CM, only 9 among a total of 75 fragments identified, were tumour specific. One hundred seventy-three fragments were identified in the portal sera of patients: one was positively and six fragments were negatively correlated with DM.
Conclusions: Unlike liver GAPDH, NO appears to be involved in PC associated DM. In portal sera, the absence, rather than the presence, of specific fragments, appears to be correlated with the development of DM.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15115683</pmid><doi>10.1016/j.cccn.2003.12.021</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Aged Aged, 80 and over Biomarkers - analysis Cell Line, Tumor Cells, Cultured Diabetes Complications - blood Diabetes Complications - genetics Diabetes Complications - metabolism Diabetes mellitus Female Glyceraldehyde-3-Phosphate Dehydrogenases - genetics Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Humans Liver - enzymology Liver - metabolism Maldi-TOF Male Middle Aged Nitric oxide Nitrites - analysis Pancreatic cancer Pancreatic Neoplasms - blood Pancreatic Neoplasms - complications Pancreatic Neoplasms - genetics Pancreatic Neoplasms - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Maldi-TOF analysis of portal sera of pancreatic cancer patients: identification of diabetogenic and antidiabetogenic peptides |
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