Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens
East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusio...
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| Veröffentlicht in: | Veterinary parasitology 2004-05, Vol.121 (1), p.53-64 |
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| creator | Kaba, Stephen A. Schaap, Dick Roode, Els C. Nene, Vishvanath Musoke, Anthony J. Vlak, Just M. van Oers, Monique M. |
| description | East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite
Theileria parva. The major sporozoite surface antigen of
T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusions to either GFP or to the baculovirus GP64 envelope glycoprotein in insect cells, which resulted in stable proteins recognized by a monoclonal specific for native p67. The immunogenicity of these fusion proteins was examined in out-bred mice and cattle. In mice, the full length p67 molecule without its signal peptide and transmembrane region, but fused to GFP (GFP:p67ΔSS) was the best immunogen followed by the C-terminus of p67 fused to GP64 (GP64:p67C). These two immunogens also provoked a high level of sero-conversion in cattle when formulated in a water-in-oil or saponin-derived adjuvant with only 100
μg of protein and a single booster. The vaccine-elicited antibodies efficiently inhibited the infectivity of
T. parva sporozoites in in vitro neutralization assays. This study demonstrated that these new baculovirus-derived p67 vaccines were highly immunogenic, and that in combination with a suitable adjuvant, they have a clear potential to induce protective immunity in cattle. |
| doi_str_mv | 10.1016/j.vetpar.2004.02.013 |
| format | Article |
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Theileria parva. The major sporozoite surface antigen of
T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusions to either GFP or to the baculovirus GP64 envelope glycoprotein in insect cells, which resulted in stable proteins recognized by a monoclonal specific for native p67. The immunogenicity of these fusion proteins was examined in out-bred mice and cattle. In mice, the full length p67 molecule without its signal peptide and transmembrane region, but fused to GFP (GFP:p67ΔSS) was the best immunogen followed by the C-terminus of p67 fused to GP64 (GP64:p67C). These two immunogens also provoked a high level of sero-conversion in cattle when formulated in a water-in-oil or saponin-derived adjuvant with only 100
μg of protein and a single booster. The vaccine-elicited antibodies efficiently inhibited the infectivity of
T. parva sporozoites in in vitro neutralization assays. This study demonstrated that these new baculovirus-derived p67 vaccines were highly immunogenic, and that in combination with a suitable adjuvant, they have a clear potential to induce protective immunity in cattle.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2004.02.013</identifier><identifier>PMID: 15110403</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adjuvants, Immunologic ; Animals ; Antibodies, Protozoan - blood ; antibody detection ; antibody formation ; Baculoviridae ; Baculoviridae - genetics ; Baculovirus ; Blotting, Western - veterinary ; Cattle ; cattle diseases ; Cattle Diseases - immunology ; Cattle Diseases - parasitology ; East Coast fever ; Enzyme-Linked Immunosorbent Assay - veterinary ; Female ; green fluorescent protein ; Green Fluorescent Proteins ; immunity ; Immunization - methods ; Immunization - veterinary ; Immunogenicity ; Luminescent Proteins ; Mice ; neutralization tests ; Neutralization Tests - veterinary ; p67 ; Protozoan Proteins - biosynthesis ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Protozoan Vaccines - genetics ; Protozoan Vaccines - immunology ; recombinant fusion proteins ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; recombinant vaccines ; seroconversion ; sporozoites ; Subunit vaccine ; subunit vaccines ; surface antigens ; Theileria parva ; Theileria parva - immunology ; Theileriasis - immunology ; Theileriasis - parasitology ; Theileriasis - prevention & control ; theileriosis ; vaccination ; vaccine adjuvants ; vaccine development ; Vaccines, Subunit - genetics ; Vaccines, Subunit - immunology</subject><ispartof>Veterinary parasitology, 2004-05, Vol.121 (1), p.53-64</ispartof><rights>2004 Elsevier B.V.</rights><rights>Copyright 2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-66bd675654419d002c219516c87fb9454e15bb22a5dbc95a8666c580ea9303d3</citedby><cites>FETCH-LOGICAL-c413t-66bd675654419d002c219516c87fb9454e15bb22a5dbc95a8666c580ea9303d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2004.02.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15110403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaba, Stephen A.</creatorcontrib><creatorcontrib>Schaap, Dick</creatorcontrib><creatorcontrib>Roode, Els C.</creatorcontrib><creatorcontrib>Nene, Vishvanath</creatorcontrib><creatorcontrib>Musoke, Anthony J.</creatorcontrib><creatorcontrib>Vlak, Just M.</creatorcontrib><creatorcontrib>van Oers, Monique M.</creatorcontrib><title>Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite
Theileria parva. The major sporozoite surface antigen of
T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusions to either GFP or to the baculovirus GP64 envelope glycoprotein in insect cells, which resulted in stable proteins recognized by a monoclonal specific for native p67. The immunogenicity of these fusion proteins was examined in out-bred mice and cattle. In mice, the full length p67 molecule without its signal peptide and transmembrane region, but fused to GFP (GFP:p67ΔSS) was the best immunogen followed by the C-terminus of p67 fused to GP64 (GP64:p67C). These two immunogens also provoked a high level of sero-conversion in cattle when formulated in a water-in-oil or saponin-derived adjuvant with only 100
μg of protein and a single booster. The vaccine-elicited antibodies efficiently inhibited the infectivity of
T. parva sporozoites in in vitro neutralization assays. This study demonstrated that these new baculovirus-derived p67 vaccines were highly immunogenic, and that in combination with a suitable adjuvant, they have a clear potential to induce protective immunity in cattle.</description><subject>Adjuvants, Immunologic</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>antibody detection</subject><subject>antibody formation</subject><subject>Baculoviridae</subject><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>Blotting, Western - veterinary</subject><subject>Cattle</subject><subject>cattle diseases</subject><subject>Cattle Diseases - immunology</subject><subject>Cattle Diseases - parasitology</subject><subject>East Coast fever</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Female</subject><subject>green fluorescent protein</subject><subject>Green Fluorescent Proteins</subject><subject>immunity</subject><subject>Immunization - methods</subject><subject>Immunization - veterinary</subject><subject>Immunogenicity</subject><subject>Luminescent Proteins</subject><subject>Mice</subject><subject>neutralization tests</subject><subject>Neutralization Tests - veterinary</subject><subject>p67</subject><subject>Protozoan Proteins - biosynthesis</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Protozoan Vaccines - genetics</subject><subject>Protozoan Vaccines - immunology</subject><subject>recombinant fusion proteins</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>recombinant vaccines</subject><subject>seroconversion</subject><subject>sporozoites</subject><subject>Subunit vaccine</subject><subject>subunit vaccines</subject><subject>surface antigens</subject><subject>Theileria parva</subject><subject>Theileria parva - immunology</subject><subject>Theileriasis - immunology</subject><subject>Theileriasis - parasitology</subject><subject>Theileriasis - prevention & control</subject><subject>theileriosis</subject><subject>vaccination</subject><subject>vaccine adjuvants</subject><subject>vaccine development</subject><subject>Vaccines, Subunit - genetics</subject><subject>Vaccines, Subunit - immunology</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAUhS1ERaeFf4AgK3ZJr9_JBglV9CFVYsGwYGU59k3xKI_BTiL13-NRRmIHG_vK-s65x4eQ9xQqClTdHKoV56ONFQMQFbAKKH9FdrTWvGRSwmuyAw6iFED1JblK6QAZBKXfkEsqKc0z35Gfj8MxTiv6IgzDMk7POAYX5pdi6ooxv_dFa93ST2uISyo9xnBi978w9Hm2RQ6w5lPpIi3tMoa5sOMcskt6Sy462yd8d76vyf7u6_72oXz6dv94--WpdILyuVSq9UpLJYWgjQdgjtFGUuVq3bWNkAKpbFvGrPSta6StlVJO1oC24cA9vyafNtv8jd8LptkMITnsezvitCSjaV1zIfV_QarzWknrDIoNdHFKKWJnjjEMNr4YCuZUvTmYrXpzqt4AM7n6LPtw9l_aAf1f0bnrDHzcgM5Oxj7HkMyP7yxLAZqcsBaZ-LwRmPtaA0aTXMDRoQ8R3Wz8FP6d4Q_-H6AM</recordid><startdate>20040507</startdate><enddate>20040507</enddate><creator>Kaba, Stephen A.</creator><creator>Schaap, Dick</creator><creator>Roode, Els C.</creator><creator>Nene, Vishvanath</creator><creator>Musoke, Anthony J.</creator><creator>Vlak, Just M.</creator><creator>van Oers, Monique M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20040507</creationdate><title>Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens</title><author>Kaba, Stephen A. ; Schaap, Dick ; Roode, Els C. ; Nene, Vishvanath ; Musoke, Anthony J. ; Vlak, Just M. ; van Oers, Monique M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-66bd675654419d002c219516c87fb9454e15bb22a5dbc95a8666c580ea9303d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adjuvants, Immunologic</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>antibody detection</topic><topic>antibody formation</topic><topic>Baculoviridae</topic><topic>Baculoviridae - genetics</topic><topic>Baculovirus</topic><topic>Blotting, Western - veterinary</topic><topic>Cattle</topic><topic>cattle diseases</topic><topic>Cattle Diseases - immunology</topic><topic>Cattle Diseases - parasitology</topic><topic>East Coast fever</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Female</topic><topic>green fluorescent protein</topic><topic>Green Fluorescent Proteins</topic><topic>immunity</topic><topic>Immunization - methods</topic><topic>Immunization - veterinary</topic><topic>Immunogenicity</topic><topic>Luminescent Proteins</topic><topic>Mice</topic><topic>neutralization tests</topic><topic>Neutralization Tests - veterinary</topic><topic>p67</topic><topic>Protozoan Proteins - biosynthesis</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Protozoan Vaccines - genetics</topic><topic>Protozoan Vaccines - immunology</topic><topic>recombinant fusion proteins</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>recombinant vaccines</topic><topic>seroconversion</topic><topic>sporozoites</topic><topic>Subunit vaccine</topic><topic>subunit vaccines</topic><topic>surface antigens</topic><topic>Theileria parva</topic><topic>Theileria parva - immunology</topic><topic>Theileriasis - immunology</topic><topic>Theileriasis - parasitology</topic><topic>Theileriasis - prevention & control</topic><topic>theileriosis</topic><topic>vaccination</topic><topic>vaccine adjuvants</topic><topic>vaccine development</topic><topic>Vaccines, Subunit - genetics</topic><topic>Vaccines, Subunit - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaba, Stephen A.</creatorcontrib><creatorcontrib>Schaap, Dick</creatorcontrib><creatorcontrib>Roode, Els C.</creatorcontrib><creatorcontrib>Nene, Vishvanath</creatorcontrib><creatorcontrib>Musoke, Anthony J.</creatorcontrib><creatorcontrib>Vlak, Just M.</creatorcontrib><creatorcontrib>van Oers, Monique M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaba, Stephen A.</au><au>Schaap, Dick</au><au>Roode, Els C.</au><au>Nene, Vishvanath</au><au>Musoke, Anthony J.</au><au>Vlak, Just M.</au><au>van Oers, Monique M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2004-05-07</date><risdate>2004</risdate><volume>121</volume><issue>1</issue><spage>53</spage><epage>64</epage><pages>53-64</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite
Theileria parva. The major sporozoite surface antigen of
T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusions to either GFP or to the baculovirus GP64 envelope glycoprotein in insect cells, which resulted in stable proteins recognized by a monoclonal specific for native p67. The immunogenicity of these fusion proteins was examined in out-bred mice and cattle. In mice, the full length p67 molecule without its signal peptide and transmembrane region, but fused to GFP (GFP:p67ΔSS) was the best immunogen followed by the C-terminus of p67 fused to GP64 (GP64:p67C). These two immunogens also provoked a high level of sero-conversion in cattle when formulated in a water-in-oil or saponin-derived adjuvant with only 100
μg of protein and a single booster. The vaccine-elicited antibodies efficiently inhibited the infectivity of
T. parva sporozoites in in vitro neutralization assays. This study demonstrated that these new baculovirus-derived p67 vaccines were highly immunogenic, and that in combination with a suitable adjuvant, they have a clear potential to induce protective immunity in cattle.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15110403</pmid><doi>10.1016/j.vetpar.2004.02.013</doi><tpages>12</tpages></addata></record> |
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| ispartof | Veterinary parasitology, 2004-05, Vol.121 (1), p.53-64 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adjuvants, Immunologic Animals Antibodies, Protozoan - blood antibody detection antibody formation Baculoviridae Baculoviridae - genetics Baculovirus Blotting, Western - veterinary Cattle cattle diseases Cattle Diseases - immunology Cattle Diseases - parasitology East Coast fever Enzyme-Linked Immunosorbent Assay - veterinary Female green fluorescent protein Green Fluorescent Proteins immunity Immunization - methods Immunization - veterinary Immunogenicity Luminescent Proteins Mice neutralization tests Neutralization Tests - veterinary p67 Protozoan Proteins - biosynthesis Protozoan Proteins - genetics Protozoan Proteins - immunology Protozoan Vaccines - genetics Protozoan Vaccines - immunology recombinant fusion proteins Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology recombinant vaccines seroconversion sporozoites Subunit vaccine subunit vaccines surface antigens Theileria parva Theileria parva - immunology Theileriasis - immunology Theileriasis - parasitology Theileriasis - prevention & control theileriosis vaccination vaccine adjuvants vaccine development Vaccines, Subunit - genetics Vaccines, Subunit - immunology |
| title | Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens |
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