Human monocyte scavenger receptors are pattern recognition receptors for (1→3)-β-D-glucans
Glucans are cell wall constituents of fungi and bacteria that bind to pattern recognition receptors and modulate innate immunity, in part, by macrophage activation. We used surface plasmon resonance to examine the binding of glucans, differing in fine structure and charge density, to scavenger recep...
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| Veröffentlicht in: | Journal of leukocyte biology 2002-07, Vol.72 (1), p.140-146 |
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| container_title | Journal of leukocyte biology |
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| creator | Rice, Peter J. Kelley, Jim L. Kogan, Grigorij Ensley, Harry E. Kalbfleisch, John H. Browder, I. William Williams, David L. |
| description | Glucans are cell wall constituents of fungi and bacteria that bind to pattern recognition receptors and modulate innate immunity, in part, by macrophage activation. We used surface plasmon resonance to examine the binding of glucans, differing in fine structure and charge density, to scavenger receptors on membranes isolated from human monocyte U937 cells. Experiments were performed at 25°C using a biosensor surface with immobilized acetylated low density lipoprotein (AcLDL). Inhibition of the binding by polyinosinic acid, but not polycytidylic acid, confirmed the interaction of scavenger receptors. Competition studies showed that there are at least two AcLDL binding sites on human U937 cells. Glucan phosphate interacts with all sites, and the CM‐glucans and laminarin interact with a subset of sites. Polymer charge has a dramatic effect on the affinity of glucans with macrophage scavenger receptors. However, it is also clear that human monocyte scavenger receptors recognize the basic glucan structure independent of charge. |
| doi_str_mv | 10.1189/jlb.72.1.140 |
| format | Article |
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Glucan phosphate interacts with all sites, and the CM‐glucans and laminarin interact with a subset of sites. Polymer charge has a dramatic effect on the affinity of glucans with macrophage scavenger receptors. However, it is also clear that human monocyte scavenger receptors recognize the basic glucan structure independent of charge.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1189/jlb.72.1.140</identifier><identifier>PMID: 12101273</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>acetylated LDL ; beta-Glucans ; binding ; Binding Sites ; Binding, Competitive ; Carboxymethylcellulose Sodium - metabolism ; Cell Adhesion Molecules ; Cell Membrane - metabolism ; Glucans - chemistry ; Glucans - metabolism ; Humans ; Lipoproteins, LDL - metabolism ; macrophage ; Monocytes - metabolism ; Poly C - metabolism ; Poly I - metabolism ; Polysaccharides - metabolism ; Receptors, Immunologic - chemistry ; Receptors, Immunologic - metabolism ; Receptors, LDL - chemistry ; Receptors, LDL - metabolism ; Receptors, Scavenger ; Sizofiran - metabolism ; surface plasmon resonance ; U937 Cells</subject><ispartof>Journal of leukocyte biology, 2002-07, Vol.72 (1), p.140-146</ispartof><rights>2002 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4280-6ae009367473c6dc6f90599a0aaa7b4e723f70a2c165d7406baae210ffd0b6253</citedby><cites>FETCH-LOGICAL-c4280-6ae009367473c6dc6f90599a0aaa7b4e723f70a2c165d7406baae210ffd0b6253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1189%2Fjlb.72.1.140$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1189%2Fjlb.72.1.140$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12101273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rice, Peter J.</creatorcontrib><creatorcontrib>Kelley, Jim L.</creatorcontrib><creatorcontrib>Kogan, Grigorij</creatorcontrib><creatorcontrib>Ensley, Harry E.</creatorcontrib><creatorcontrib>Kalbfleisch, John H.</creatorcontrib><creatorcontrib>Browder, I. William</creatorcontrib><creatorcontrib>Williams, David L.</creatorcontrib><title>Human monocyte scavenger receptors are pattern recognition receptors for (1→3)-β-D-glucans</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Glucans are cell wall constituents of fungi and bacteria that bind to pattern recognition receptors and modulate innate immunity, in part, by macrophage activation. We used surface plasmon resonance to examine the binding of glucans, differing in fine structure and charge density, to scavenger receptors on membranes isolated from human monocyte U937 cells. Experiments were performed at 25°C using a biosensor surface with immobilized acetylated low density lipoprotein (AcLDL). Inhibition of the binding by polyinosinic acid, but not polycytidylic acid, confirmed the interaction of scavenger receptors. Competition studies showed that there are at least two AcLDL binding sites on human U937 cells. Glucan phosphate interacts with all sites, and the CM‐glucans and laminarin interact with a subset of sites. Polymer charge has a dramatic effect on the affinity of glucans with macrophage scavenger receptors. However, it is also clear that human monocyte scavenger receptors recognize the basic glucan structure independent of charge.</description><subject>acetylated LDL</subject><subject>beta-Glucans</subject><subject>binding</subject><subject>Binding Sites</subject><subject>Binding, Competitive</subject><subject>Carboxymethylcellulose Sodium - metabolism</subject><subject>Cell Adhesion Molecules</subject><subject>Cell Membrane - metabolism</subject><subject>Glucans - chemistry</subject><subject>Glucans - metabolism</subject><subject>Humans</subject><subject>Lipoproteins, LDL - metabolism</subject><subject>macrophage</subject><subject>Monocytes - metabolism</subject><subject>Poly C - metabolism</subject><subject>Poly I - metabolism</subject><subject>Polysaccharides - metabolism</subject><subject>Receptors, Immunologic - chemistry</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Receptors, LDL - chemistry</subject><subject>Receptors, LDL - metabolism</subject><subject>Receptors, Scavenger</subject><subject>Sizofiran - metabolism</subject><subject>surface plasmon resonance</subject><subject>U937 Cells</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9u1DAQhy1ERbeFG2eUCwgksp2xvbFzhNK_WolLOSLL8U62qZJ4sROifYE-AI_SB-EheJKmyopyoqeRRt98M_Nj7DXCHFHnRzd1MVd8jnOU8IzNMBc6FZkSz9kMlMR0IQH22UGMNwAgeAYv2D5yBORKzNj3876xbdL41rttR0l09ie1awpJIEebzoeY2EDJxnYdhfah69dt1VW-_YcofUje45_bX-JD-vsu_ZKu697ZNr5ke6WtI73a1UP27fTk6vg8XX49uzj-tEyd5BrSzBJAPh4tlXDZymVlDos8t2CtVYUkxUWpwHKH2WKlJGSFtTS-UJYrKDK-EIfs3eTdBP-jp9iZpoqO6tq25PtoFGrNhdZPgqil1BLlCH6cQBd8jIFKswlVY8PWIJiH3M2Yu1HcoBlzH_E3O29fNLR6hHdBjwBOwFDVtP2vzFwuP8MkfTvNXFfr66EKZGJj63pcwc0wDH-X3wNkc5vD</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Rice, Peter J.</creator><creator>Kelley, Jim L.</creator><creator>Kogan, Grigorij</creator><creator>Ensley, Harry E.</creator><creator>Kalbfleisch, John H.</creator><creator>Browder, I. William</creator><creator>Williams, David L.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Human monocyte scavenger receptors are pattern recognition receptors for (1→3)-β-D-glucans</title><author>Rice, Peter J. ; Kelley, Jim L. ; Kogan, Grigorij ; Ensley, Harry E. ; Kalbfleisch, John H. ; Browder, I. William ; Williams, David L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4280-6ae009367473c6dc6f90599a0aaa7b4e723f70a2c165d7406baae210ffd0b6253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>acetylated LDL</topic><topic>beta-Glucans</topic><topic>binding</topic><topic>Binding Sites</topic><topic>Binding, Competitive</topic><topic>Carboxymethylcellulose Sodium - metabolism</topic><topic>Cell Adhesion Molecules</topic><topic>Cell Membrane - metabolism</topic><topic>Glucans - chemistry</topic><topic>Glucans - metabolism</topic><topic>Humans</topic><topic>Lipoproteins, LDL - metabolism</topic><topic>macrophage</topic><topic>Monocytes - metabolism</topic><topic>Poly C - metabolism</topic><topic>Poly I - metabolism</topic><topic>Polysaccharides - metabolism</topic><topic>Receptors, Immunologic - chemistry</topic><topic>Receptors, Immunologic - metabolism</topic><topic>Receptors, LDL - chemistry</topic><topic>Receptors, LDL - metabolism</topic><topic>Receptors, Scavenger</topic><topic>Sizofiran - metabolism</topic><topic>surface plasmon resonance</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rice, Peter J.</creatorcontrib><creatorcontrib>Kelley, Jim L.</creatorcontrib><creatorcontrib>Kogan, Grigorij</creatorcontrib><creatorcontrib>Ensley, Harry E.</creatorcontrib><creatorcontrib>Kalbfleisch, John H.</creatorcontrib><creatorcontrib>Browder, I. 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William</au><au>Williams, David L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human monocyte scavenger receptors are pattern recognition receptors for (1→3)-β-D-glucans</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>72</volume><issue>1</issue><spage>140</spage><epage>146</epage><pages>140-146</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>Glucans are cell wall constituents of fungi and bacteria that bind to pattern recognition receptors and modulate innate immunity, in part, by macrophage activation. We used surface plasmon resonance to examine the binding of glucans, differing in fine structure and charge density, to scavenger receptors on membranes isolated from human monocyte U937 cells. Experiments were performed at 25°C using a biosensor surface with immobilized acetylated low density lipoprotein (AcLDL). Inhibition of the binding by polyinosinic acid, but not polycytidylic acid, confirmed the interaction of scavenger receptors. Competition studies showed that there are at least two AcLDL binding sites on human U937 cells. Glucan phosphate interacts with all sites, and the CM‐glucans and laminarin interact with a subset of sites. Polymer charge has a dramatic effect on the affinity of glucans with macrophage scavenger receptors. However, it is also clear that human monocyte scavenger receptors recognize the basic glucan structure independent of charge.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>12101273</pmid><doi>10.1189/jlb.72.1.140</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | acetylated LDL beta-Glucans binding Binding Sites Binding, Competitive Carboxymethylcellulose Sodium - metabolism Cell Adhesion Molecules Cell Membrane - metabolism Glucans - chemistry Glucans - metabolism Humans Lipoproteins, LDL - metabolism macrophage Monocytes - metabolism Poly C - metabolism Poly I - metabolism Polysaccharides - metabolism Receptors, Immunologic - chemistry Receptors, Immunologic - metabolism Receptors, LDL - chemistry Receptors, LDL - metabolism Receptors, Scavenger Sizofiran - metabolism surface plasmon resonance U937 Cells |
| title | Human monocyte scavenger receptors are pattern recognition receptors for (1→3)-β-D-glucans |
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