Development of an inducible system to control and easily monitor gene expression in Lactococcus lactis
This report describes the implementation and use of a maltose-inducible system for regulated gene expression in Lactococcus lactis. The system was established using Green Fluorescent Protein as reporter. The transcription of a gene of interest from the inducible promoter of pLS1RGFP plasmid vector c...
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| Veröffentlicht in: | Plasmid 2004-05, Vol.51 (3), p.256-264 |
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| container_title | Plasmid |
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| creator | Viegas, S.C. Fernández de Palencia, P. Amblar, M. Arraiano, C.M. López, P. |
| description | This report describes the implementation and use of a maltose-inducible system for regulated gene expression in
Lactococcus lactis. The system was established using Green Fluorescent Protein as reporter. The transcription of a gene of interest from the inducible promoter of pLS1RGFP plasmid vector can be easily monitored by fluorescence spectroscopy and microscopy. As an example, the lactococcal ribonuclease III was overproduced in an active form. |
| doi_str_mv | 10.1016/j.plasmid.2004.02.004 |
| format | Article |
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| ispartof | Plasmid, 2004-05, Vol.51 (3), p.256-264 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Blotting, Western DNA Primers Expression vector Gene Expression Regulation, Bacterial Genes, Reporter - genetics Genetic Vectors - genetics Green Fluorescent Proteins Lactococcus lactis Lactococcus lactis - genetics Luminescent Proteins Maltose Maltose-induction system Microscopy, Fluorescence Plasmids - genetics Regulation of gene expression Ribonuclease III Ribonuclease III - genetics |
| title | Development of an inducible system to control and easily monitor gene expression in Lactococcus lactis |
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