Determination of cidofovir in both skin layers and percutaneous penetration samples by HPLC
The aim of this study was to develop a direct, simple and rapid high performance liquid chromatographic method for the determination of cidofovir in both skin layers and percutaneous penetration experiments. Samples were chromatographed on a reversed phase encapped column 250×4 mm C 8 LiChrospher Se...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2002-07, Vol.29 (5), p.819-826 |
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| creator | Santoyo, S de Jalón, E.G Campanero, M.A Ygartua, P |
| description | The aim of this study was to develop a direct, simple and rapid high performance liquid chromatographic method for the determination of cidofovir in both skin layers and percutaneous penetration experiments. Samples were chromatographed on a reversed phase encapped column 250×4 mm C
8 LiChrospher Select B. The phase mobile consisted on 3% of acetonitrile and 97% of 1.5 mM of tetrabutylammonium dihydrogen phosphate (TADP) and 3.5 mM of disodium hydrogenphosphate adjusted to pH 6. Detection was at 274 nm and the run time was 14 min. The limit of detection was 0.06 μg/ml. The detector response was found to be linear in the concentration range 0.1–10 μg/ml. This assay is a selective, sensitive and reproducible method for the quantification of cidofovir in skin layers and in the receptor compartment of Franz-type diffusion cells after percutaneous studies. |
| doi_str_mv | 10.1016/S0731-7085(02)00188-7 |
| format | Article |
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8 LiChrospher Select B. The phase mobile consisted on 3% of acetonitrile and 97% of 1.5 mM of tetrabutylammonium dihydrogen phosphate (TADP) and 3.5 mM of disodium hydrogenphosphate adjusted to pH 6. Detection was at 274 nm and the run time was 14 min. The limit of detection was 0.06 μg/ml. The detector response was found to be linear in the concentration range 0.1–10 μg/ml. This assay is a selective, sensitive and reproducible method for the quantification of cidofovir in skin layers and in the receptor compartment of Franz-type diffusion cells after percutaneous studies.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(02)00188-7</identifier><identifier>PMID: 12093514</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Antiviral Agents - pharmacokinetics ; Biological and medical sciences ; Calibration ; Chromatography, High Pressure Liquid - methods ; Cidofovir ; Cytosine - analogs & derivatives ; Cytosine - pharmacokinetics ; General pharmacology ; HPLC ; Humans ; Hydrogen-Ion Concentration ; Medical sciences ; Organophosphonates ; Organophosphorus Compounds - pharmacokinetics ; Percutaneous permeation ; Permeability ; Pharmacology. Drug treatments ; Reproducibility of Results ; Sensitivity and Specificity ; Skin - metabolism ; Skin Absorption ; Skin recovery ; Spectrophotometry, Ultraviolet</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2002-07, Vol.29 (5), p.819-826</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-3029aaa9a808656947d8ba362e2ec97046cc890a5891514261590e54dec546c73</citedby><cites>FETCH-LOGICAL-c422t-3029aaa9a808656947d8ba362e2ec97046cc890a5891514261590e54dec546c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0731-7085(02)00188-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13763989$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12093514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santoyo, S</creatorcontrib><creatorcontrib>de Jalón, E.G</creatorcontrib><creatorcontrib>Campanero, M.A</creatorcontrib><creatorcontrib>Ygartua, P</creatorcontrib><title>Determination of cidofovir in both skin layers and percutaneous penetration samples by HPLC</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>The aim of this study was to develop a direct, simple and rapid high performance liquid chromatographic method for the determination of cidofovir in both skin layers and percutaneous penetration experiments. Samples were chromatographed on a reversed phase encapped column 250×4 mm C
8 LiChrospher Select B. The phase mobile consisted on 3% of acetonitrile and 97% of 1.5 mM of tetrabutylammonium dihydrogen phosphate (TADP) and 3.5 mM of disodium hydrogenphosphate adjusted to pH 6. Detection was at 274 nm and the run time was 14 min. The limit of detection was 0.06 μg/ml. The detector response was found to be linear in the concentration range 0.1–10 μg/ml. This assay is a selective, sensitive and reproducible method for the quantification of cidofovir in skin layers and in the receptor compartment of Franz-type diffusion cells after percutaneous studies.</description><subject>Analysis</subject><subject>Antiviral Agents - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cidofovir</subject><subject>Cytosine - analogs & derivatives</subject><subject>Cytosine - pharmacokinetics</subject><subject>General pharmacology</subject><subject>HPLC</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical sciences</subject><subject>Organophosphonates</subject><subject>Organophosphorus Compounds - pharmacokinetics</subject><subject>Percutaneous permeation</subject><subject>Permeability</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Skin - metabolism</subject><subject>Skin Absorption</subject><subject>Skin recovery</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo7uzqT1ByUdZDa-U7OYmMHysMKKggeAiZdDVGuzuzSc_C_Ht7tgf3uKeqIk9VXh5CnjF4zYDpN9_ACNYYsOoS-CsAZm1jHpAVs0Y0XMufD8nqP3JGzmv9AwCKOfmYnDEOTigmV-TXe5ywDGkMU8ojzR2Nqc1dvkmFppFu8_Sb1r9z14cDlkrD2NIdlrifwoh5X-dhxKks2zUMux4r3R7o1dfN-gl51IW-4tNTvSA_Pn74vr5qNl8-fV6_2zRRcj41ArgLIbhgwWqlnTSt3QahOXKMzoDUMVoHQVnH5sxcM-UAlWwxqvnNiAvycrm7K_l6j3XyQ6oR-36J6M3sRDul7gWZlUZacQTVAsaSay3Y-V1JQygHz8Af9ftb_f7o1gP3t_r9Mcnz0wf77YDt3dbJ9wy8OAGhxtB3JYwx1TtOGC2cdTP3duFw9naTsPgaE44R21QwTr7N6Z4o_wCKBKDB</recordid><startdate>20020731</startdate><enddate>20020731</enddate><creator>Santoyo, S</creator><creator>de Jalón, E.G</creator><creator>Campanero, M.A</creator><creator>Ygartua, P</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020731</creationdate><title>Determination of cidofovir in both skin layers and percutaneous penetration samples by HPLC</title><author>Santoyo, S ; de Jalón, E.G ; Campanero, M.A ; Ygartua, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-3029aaa9a808656947d8ba362e2ec97046cc890a5891514261590e54dec546c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analysis</topic><topic>Antiviral Agents - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cidofovir</topic><topic>Cytosine - analogs & derivatives</topic><topic>Cytosine - pharmacokinetics</topic><topic>General pharmacology</topic><topic>HPLC</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical sciences</topic><topic>Organophosphonates</topic><topic>Organophosphorus Compounds - pharmacokinetics</topic><topic>Percutaneous permeation</topic><topic>Permeability</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Skin - metabolism</topic><topic>Skin Absorption</topic><topic>Skin recovery</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santoyo, S</creatorcontrib><creatorcontrib>de Jalón, E.G</creatorcontrib><creatorcontrib>Campanero, M.A</creatorcontrib><creatorcontrib>Ygartua, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santoyo, S</au><au>de Jalón, E.G</au><au>Campanero, M.A</au><au>Ygartua, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of cidofovir in both skin layers and percutaneous penetration samples by HPLC</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2002-07-31</date><risdate>2002</risdate><volume>29</volume><issue>5</issue><spage>819</spage><epage>826</epage><pages>819-826</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>The aim of this study was to develop a direct, simple and rapid high performance liquid chromatographic method for the determination of cidofovir in both skin layers and percutaneous penetration experiments. Samples were chromatographed on a reversed phase encapped column 250×4 mm C
8 LiChrospher Select B. The phase mobile consisted on 3% of acetonitrile and 97% of 1.5 mM of tetrabutylammonium dihydrogen phosphate (TADP) and 3.5 mM of disodium hydrogenphosphate adjusted to pH 6. Detection was at 274 nm and the run time was 14 min. The limit of detection was 0.06 μg/ml. The detector response was found to be linear in the concentration range 0.1–10 μg/ml. This assay is a selective, sensitive and reproducible method for the quantification of cidofovir in skin layers and in the receptor compartment of Franz-type diffusion cells after percutaneous studies.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12093514</pmid><doi>10.1016/S0731-7085(02)00188-7</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Antiviral Agents - pharmacokinetics Biological and medical sciences Calibration Chromatography, High Pressure Liquid - methods Cidofovir Cytosine - analogs & derivatives Cytosine - pharmacokinetics General pharmacology HPLC Humans Hydrogen-Ion Concentration Medical sciences Organophosphonates Organophosphorus Compounds - pharmacokinetics Percutaneous permeation Permeability Pharmacology. Drug treatments Reproducibility of Results Sensitivity and Specificity Skin - metabolism Skin Absorption Skin recovery Spectrophotometry, Ultraviolet |
| title | Determination of cidofovir in both skin layers and percutaneous penetration samples by HPLC |
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