Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry
We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). T...
Gespeichert in:
| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-06, Vol.805 (1), p.113-117 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 117 |
|---|---|
| container_issue | 1 |
| container_start_page | 113 |
| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| container_volume | 805 |
| creator | Kage, Shigetoshi Kudo, Keiko Ikeda, Hideaki Ikeda, Noriaki |
| description | We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography–mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02
mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0
mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10%. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases. |
| doi_str_mv | 10.1016/j.jchromb.2004.02.029 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71875767</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1570023204001722</els_id><sourcerecordid>71875767</sourcerecordid><originalsourceid>FETCH-LOGICAL-c486t-e1e2f8195a045d8dfd0cf68a6e62e9b566dd1d6ccce6a9fffcc50c5890600b5c3</originalsourceid><addsrcrecordid>eNqFkMGKFDEQhoMo7rr6CEoueusx6Z4k3SeRRVdhwYMK3kI6qcxk6CRjkl6Zm-Aj-Ib7JGZmGvQmFKSgvkr-fAg9p2RFCeWvd6ud3qbox1VLyHpF2lrDA3RJe9E1neDfHtaeCdKQtmsv0JOcd4RQQUT3GF1QRmnH1vwS_frs_DwVFSDOGRsokLwLqrgYcLTYxuRVAayCwUpDOfYu4B_bOAEepxjNaTQnFwDbGgdvZ69CxnN2YYM3KuNTSlXiJqn99nD_87dXOeO8B13qAEo6PEWPrJoyPFvOK_T1_bsv1x-a2083H6_f3jZ63fPSAIXW9nRgiqyZ6Y01RFveKw68hWFknBtDDddaA1eDtVZrRjTrB8IJGZnurtCr8737FL_PkIv0LmuYpvPvpajumOCiguwM6hRzTmDlPjmv0kFSIo_25U4u9uXRviRtraHuvVgemEcP5u_WorsCLxdAZa0mm1TQLv_DCUr7U4A3Zw6qjjsHSWbtIGgwLlVv0kT3nyh_AGzJq0Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71875767</pqid></control><display><type>article</type><title>Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Kage, Shigetoshi ; Kudo, Keiko ; Ikeda, Hideaki ; Ikeda, Noriaki</creator><creatorcontrib>Kage, Shigetoshi ; Kudo, Keiko ; Ikeda, Hideaki ; Ikeda, Noriaki</creatorcontrib><description>We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography–mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02
mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0
mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10%. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2004.02.029</identifier><identifier>PMID: 15113546</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetate ; Acetates - blood ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Formate ; Formates - blood ; Fundamental and applied biological sciences. Psychology ; Gas Chromatography-Mass Spectrometry - methods ; General pharmacology ; Humans ; Medical sciences ; Pharmacology. Drug treatments ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2004-06, Vol.805 (1), p.113-117</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-e1e2f8195a045d8dfd0cf68a6e62e9b566dd1d6ccce6a9fffcc50c5890600b5c3</citedby><cites>FETCH-LOGICAL-c486t-e1e2f8195a045d8dfd0cf68a6e62e9b566dd1d6ccce6a9fffcc50c5890600b5c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2004.02.029$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15711867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15113546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kage, Shigetoshi</creatorcontrib><creatorcontrib>Kudo, Keiko</creatorcontrib><creatorcontrib>Ikeda, Hideaki</creatorcontrib><creatorcontrib>Ikeda, Noriaki</creatorcontrib><title>Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography–mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02
mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0
mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10%. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases.</description><subject>Acetate</subject><subject>Acetates - blood</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Formate</subject><subject>Formates - blood</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMGKFDEQhoMo7rr6CEoueusx6Z4k3SeRRVdhwYMK3kI6qcxk6CRjkl6Zm-Aj-Ib7JGZmGvQmFKSgvkr-fAg9p2RFCeWvd6ud3qbox1VLyHpF2lrDA3RJe9E1neDfHtaeCdKQtmsv0JOcd4RQQUT3GF1QRmnH1vwS_frs_DwVFSDOGRsokLwLqrgYcLTYxuRVAayCwUpDOfYu4B_bOAEepxjNaTQnFwDbGgdvZ69CxnN2YYM3KuNTSlXiJqn99nD_87dXOeO8B13qAEo6PEWPrJoyPFvOK_T1_bsv1x-a2083H6_f3jZ63fPSAIXW9nRgiqyZ6Y01RFveKw68hWFknBtDDddaA1eDtVZrRjTrB8IJGZnurtCr8737FL_PkIv0LmuYpvPvpajumOCiguwM6hRzTmDlPjmv0kFSIo_25U4u9uXRviRtraHuvVgemEcP5u_WorsCLxdAZa0mm1TQLv_DCUr7U4A3Zw6qjjsHSWbtIGgwLlVv0kT3nyh_AGzJq0Y</recordid><startdate>20040605</startdate><enddate>20040605</enddate><creator>Kage, Shigetoshi</creator><creator>Kudo, Keiko</creator><creator>Ikeda, Hideaki</creator><creator>Ikeda, Noriaki</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040605</creationdate><title>Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry</title><author>Kage, Shigetoshi ; Kudo, Keiko ; Ikeda, Hideaki ; Ikeda, Noriaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-e1e2f8195a045d8dfd0cf68a6e62e9b566dd1d6ccce6a9fffcc50c5890600b5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acetate</topic><topic>Acetates - blood</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Formate</topic><topic>Formates - blood</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kage, Shigetoshi</creatorcontrib><creatorcontrib>Kudo, Keiko</creatorcontrib><creatorcontrib>Ikeda, Hideaki</creatorcontrib><creatorcontrib>Ikeda, Noriaki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kage, Shigetoshi</au><au>Kudo, Keiko</au><au>Ikeda, Hideaki</au><au>Ikeda, Noriaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2004-06-05</date><risdate>2004</risdate><volume>805</volume><issue>1</issue><spage>113</spage><epage>117</epage><pages>113-117</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography–mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02
mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0
mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10%. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15113546</pmid><doi>10.1016/j.jchromb.2004.02.029</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2004-06, Vol.805 (1), p.113-117 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71875767 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acetate Acetates - blood Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Formate Formates - blood Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry - methods General pharmacology Humans Medical sciences Pharmacology. Drug treatments Reference Standards Reproducibility of Results Sensitivity and Specificity |
| title | Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T19%3A10%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20determination%20of%20formate%20and%20acetate%20in%20whole%20blood%20and%20urine%20from%20humans%20using%20gas%20chromatography%E2%80%93mass%20spectrometry&rft.jtitle=Journal%20of%20chromatography.%20B,%20Analytical%20technologies%20in%20the%20biomedical%20and%20life%20sciences&rft.au=Kage,%20Shigetoshi&rft.date=2004-06-05&rft.volume=805&rft.issue=1&rft.spage=113&rft.epage=117&rft.pages=113-117&rft.issn=1570-0232&rft.eissn=1873-376X&rft_id=info:doi/10.1016/j.jchromb.2004.02.029&rft_dat=%3Cproquest_cross%3E71875767%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71875767&rft_id=info:pmid/15113546&rft_els_id=S1570023204001722&rfr_iscdi=true |