Design and Use of 16S Ribosomal DNA-Directed Primers in Competitive PCRs to Enumerate Proteolytic Bacteria in the Rumen
Competitive Polymerase Chain Reaction primers were designed for Streptococcus, B. fibrisolvens, P. bryantii, Eubacterium sp., Prevotella, and a universal primer for the eubacteria. DNA was extracted from rumen contents collected from eight dairy cows fed four diets: adequate nitrogen, adequate nitro...
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| Veröffentlicht in: | Microbial ecology 2002-03, Vol.43 (2), p.259-270 |
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| creator | REILLY, K CARRUTHERS, V. R ATTWOOD, G. T |
| description | Competitive Polymerase Chain Reaction primers were designed for Streptococcus, B. fibrisolvens, P. bryantii, Eubacterium sp., Prevotella, and a universal primer for the eubacteria. DNA was extracted from rumen contents collected from eight dairy cows fed four diets: adequate nitrogen, adequate nitrogen plus carbohydrate, low nitrogen, and low nitrogen plus carbohydrate. B. fibrisolvens was significantly higher on the adequate nitrogen plus carbohydrate and the low nitrogen plus carbohydrate diets compared with the other diets, while P. bryantii was significantly higher on the low nitrogen plus carbohydrate diet compared with the adequate nitrogen diet. The population of Eubacterium sp. was significantly lower on both the adequate nitrogen plus carbohydrate and low nitrogen plus carbohydrate diets. Streptococcus populations were significantly lower on the low nitrogen plus carbohydrate diet compared with all three other diets, whereas there were no significant differences in populations of Prevotella or total eubacteria on any of the diets. |
| doi_str_mv | 10.1007/s00248-001-1052-2 |
| format | Article |
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Streptococcus populations were significantly lower on the low nitrogen plus carbohydrate diet compared with all three other diets, whereas there were no significant differences in populations of Prevotella or total eubacteria on any of the diets.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-001-1052-2</identifier><identifier>PMID: 12023733</identifier><identifier>CODEN: MCBEBU</identifier><language>eng</language><publisher>New York, NY: Springer-Verlag New York Inc</publisher><subject>Animal Feed ; Animal, plant and microbial ecology ; Animals ; Bacteria ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Cattle ; Diet ; Dietary Carbohydrates ; DNA Primers ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; Eubacterium ; Eubacterium - genetics ; Eubacterium - isolation & purification ; Female ; Fundamental and applied biological sciences. Psychology ; Microbial ecology ; Microbiology ; Nitrogen ; Normal microflora of man and animals. Rumen ; Pastures ; Polymerase Chain Reaction - methods ; Prevotella ; Prevotella - genetics ; Prevotella - isolation & purification ; Ribosomal DNA ; RNA, Ribosomal, 16S - genetics ; Rumen ; Rumen - microbiology ; Rumen bacteria ; Streptococcus ; Streptococcus - genetics ; Streptococcus - isolation & purification</subject><ispartof>Microbial ecology, 2002-03, Vol.43 (2), p.259-270</ispartof><rights>Copyright 2002 Springer-Verlag New York Inc.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-59a7f678912d582865bf555f4c06cfd0bd4268b0f61e9591a323939cba94e92b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4287595$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4287595$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13855434$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12023733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>REILLY, K</creatorcontrib><creatorcontrib>CARRUTHERS, V. R</creatorcontrib><creatorcontrib>ATTWOOD, G. T</creatorcontrib><title>Design and Use of 16S Ribosomal DNA-Directed Primers in Competitive PCRs to Enumerate Proteolytic Bacteria in the Rumen</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><description>Competitive Polymerase Chain Reaction primers were designed for Streptococcus, B. fibrisolvens, P. bryantii, Eubacterium sp., Prevotella, and a universal primer for the eubacteria. DNA was extracted from rumen contents collected from eight dairy cows fed four diets: adequate nitrogen, adequate nitrogen plus carbohydrate, low nitrogen, and low nitrogen plus carbohydrate. B. fibrisolvens was significantly higher on the adequate nitrogen plus carbohydrate and the low nitrogen plus carbohydrate diets compared with the other diets, while P. bryantii was significantly higher on the low nitrogen plus carbohydrate diet compared with the adequate nitrogen diet. The population of Eubacterium sp. was significantly lower on both the adequate nitrogen plus carbohydrate and low nitrogen plus carbohydrate diets. Streptococcus populations were significantly lower on the low nitrogen plus carbohydrate diet compared with all three other diets, whereas there were no significant differences in populations of Prevotella or total eubacteria on any of the diets.</description><subject>Animal Feed</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Diet</subject><subject>Dietary Carbohydrates</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Eubacterium</subject><subject>Eubacterium - genetics</subject><subject>Eubacterium - isolation & purification</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Nitrogen</subject><subject>Normal microflora of man and animals. Rumen</subject><subject>Pastures</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Prevotella</subject><subject>Prevotella - genetics</subject><subject>Prevotella - isolation & purification</subject><subject>Ribosomal DNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Rumen</subject><subject>Rumen - microbiology</subject><subject>Rumen bacteria</subject><subject>Streptococcus</subject><subject>Streptococcus - genetics</subject><subject>Streptococcus - isolation & purification</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFtrVDEURoModqz-AEEkL_oWzf3yWGfqBYqW0YJvIScn0ZRzTqZJRum_N8MM9mnD3uv7YC8AXhL8jmCs3leMKdcIY4IIFhTRR2BFOKOIaP7zMVhhbARikuoz8KzW284pSdlTcEYopkwxtgJ_N6GmXwt0ywhvaoA5QiK_w20acs2zm-Dm6wXapBJ8CyO8LmkOpcK0wHWed6Gllv4EeL3eVtgyvFz2_exa35TcQp7uW_Lwg-vZktwh1X4HuO3Q8hw8iW6q4cVpnoObj5c_1p_R1bdPX9YXV8gzrhsSxqkolTaEjkJTLcUQhRCReyx9HPEwcir1gKMkwQhDHKPMMOMHZ3gwdGDn4O2xd1fy3T7UZudUfZgmt4S8r1YRrbjUqoPkCPqSay0h2l1_1pV7S7A92LZH27ZbtAfblvbM61P5fpjD-JA46e3AmxPgqndTLG7xqT5wTAvBGe_cqyN3W1su_--caiWMYP8AK4GPuQ</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>REILLY, K</creator><creator>CARRUTHERS, V. R</creator><creator>ATTWOOD, G. T</creator><general>Springer-Verlag New York Inc</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>Design and Use of 16S Ribosomal DNA-Directed Primers in Competitive PCRs to Enumerate Proteolytic Bacteria in the Rumen</title><author>REILLY, K ; CARRUTHERS, V. R ; ATTWOOD, G. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-59a7f678912d582865bf555f4c06cfd0bd4268b0f61e9591a323939cba94e92b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animal Feed</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Diet</topic><topic>Dietary Carbohydrates</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>Eubacterium</topic><topic>Eubacterium - genetics</topic><topic>Eubacterium - isolation & purification</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Nitrogen</topic><topic>Normal microflora of man and animals. Rumen</topic><topic>Pastures</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Prevotella</topic><topic>Prevotella - genetics</topic><topic>Prevotella - isolation & purification</topic><topic>Ribosomal DNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Rumen</topic><topic>Rumen - microbiology</topic><topic>Rumen bacteria</topic><topic>Streptococcus</topic><topic>Streptococcus - genetics</topic><topic>Streptococcus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>REILLY, K</creatorcontrib><creatorcontrib>CARRUTHERS, V. R</creatorcontrib><creatorcontrib>ATTWOOD, G. 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B. fibrisolvens was significantly higher on the adequate nitrogen plus carbohydrate and the low nitrogen plus carbohydrate diets compared with the other diets, while P. bryantii was significantly higher on the low nitrogen plus carbohydrate diet compared with the adequate nitrogen diet. The population of Eubacterium sp. was significantly lower on both the adequate nitrogen plus carbohydrate and low nitrogen plus carbohydrate diets. Streptococcus populations were significantly lower on the low nitrogen plus carbohydrate diet compared with all three other diets, whereas there were no significant differences in populations of Prevotella or total eubacteria on any of the diets.</abstract><cop>New York, NY</cop><pub>Springer-Verlag New York Inc</pub><pmid>12023733</pmid><doi>10.1007/s00248-001-1052-2</doi><tpages>12</tpages></addata></record> |
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| subjects | Animal Feed Animal, plant and microbial ecology Animals Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cattle Diet Dietary Carbohydrates DNA Primers DNA, Bacterial - analysis DNA, Bacterial - genetics Eubacterium Eubacterium - genetics Eubacterium - isolation & purification Female Fundamental and applied biological sciences. Psychology Microbial ecology Microbiology Nitrogen Normal microflora of man and animals. Rumen Pastures Polymerase Chain Reaction - methods Prevotella Prevotella - genetics Prevotella - isolation & purification Ribosomal DNA RNA, Ribosomal, 16S - genetics Rumen Rumen - microbiology Rumen bacteria Streptococcus Streptococcus - genetics Streptococcus - isolation & purification |
| title | Design and Use of 16S Ribosomal DNA-Directed Primers in Competitive PCRs to Enumerate Proteolytic Bacteria in the Rumen |
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