RNA interference in adult mice

RNA interference in adult mice

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nature (London) 2002-07, Vol.418 (6893), p.38-39
Hauptverfasser: McCaffrey, Anton P, Meuse, Leonard, Pham, Thu-Thao T, Conklin, Douglas S, Hannon, Gregory J, Kay, Mark A
Format: Artikel
Sprache:eng
Schlagworte:
Aging - genetics
Animals
Gene Silencing
Genes, Reporter - genetics
Hepacivirus - genetics
Humans
Liver - metabolism
Luciferases - biosynthesis
Luciferases - genetics
Mice
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
RNA, Double-Stranded - chemistry
RNA, Double-Stranded - genetics
RNA, Double-Stranded - metabolism
RNA, Small Interfering
RNA, Untranslated - chemistry
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
RNA, Viral - genetics
RNA, Viral - metabolism
Substrate Specificity
Transgenes - genetics
Viral Nonstructural Proteins - biosynthesis
Viral Nonstructural Proteins - genetics
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.
ISSN:0028-0836
1476-4687
DOI:10.1038/418038a