In vitro engineering of heart muscle: Artificial myocardial tissue
Introduction: Myocardial infarction followed by heart failure represents one of the major causes of morbidity and mortality, particularly in industrialized countries. Engineering and subsequent transplantation of contractile artificial myocardial tissue and, consequently, the replacement of ischemic...
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| creator | Kofidis, T. Akhyari, P. Boublik, J. Theodorou, P. Martin, U. Ruhparwar, A. Fischer, S. Eschenhagen, T. Kubis, H.P. Kraft, T. Leyh, R. Haverich, A. |
| description | Introduction: Myocardial infarction followed by heart failure represents one of the major causes of morbidity and mortality, particularly in industrialized countries. Engineering and subsequent transplantation of contractile artificial myocardial tissue and, consequently, the replacement of ischemic and infarcted areas of the heart provides a potential therapeutic alternative to whole organ transplantation. Methods: Artificial myocardial tissue samples were engineered by seeding neonatal rat cardiomyocytes with a commercially available 3-dimensional collagen matrix. The cellular engraftment within the artificial myocardial tissues was examined microscopically. Force development was analyzed in spontaneously beating artificial myocardial tissues, after stretching, and after pharmacologic stimulation. Moreover, electrocardiograms were recorded. Results: Artificial myocardial tissues showed continuous, rhythmic, and synchronized contractions for up to 13 weeks. Embedded cardiomyocytes were distributed equally within the 3-dimensional matrix. Application of Ca2+ and epinephrine, as well as electrical stimulation or stretching, resulted in enhanced force development. Electrocardiographic recording was possible on spontaneously beating artificial myocardial tissue samples and revealed physiologic patterns. Conclusions: Using a clinically well-established collagen matrix, contractile myocardial tissue can be engineered in vitro successfully. Mechanical and biologic properties of artificial myocardial tissue resemble native cardiac tissue. Use of artificial myocardial tissues might be a promising approach to reconstitute degenerated or failing cardiac tissue in many disease states and therefore provide a reasonable alternative to whole organ transplantation.
J Thorac Cardiovasc Surg 2002;124:63-9 |
| doi_str_mv | 10.1067/mtc.2002.121971 |
| format | Article |
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J Thorac Cardiovasc Surg 2002;124:63-9</description><identifier>ISSN: 0022-5223</identifier><identifier>EISSN: 1097-685X</identifier><identifier>DOI: 10.1067/mtc.2002.121971</identifier><identifier>PMID: 12091810</identifier><identifier>CODEN: JTCSAQ</identifier><language>eng</language><publisher>Philadelphia, PA: Mosby, Inc</publisher><subject>Animals ; Animals, Newborn ; Biological and medical sciences ; Collagen ; Diseases of the cardiovascular system ; Electric Stimulation ; Electrocardiography ; Medical sciences ; Myocardial Contraction ; Myocardium - cytology ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Rats ; Rats, Wistar ; Tissue Engineering - methods</subject><ispartof>The Journal of thoracic and cardiovascular surgery, 2002-07, Vol.124 (1), p.63-69</ispartof><rights>2002 American Association for Thoracic Surgery</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-ee2f8d3c58dfbf5fa9b2cde322af9b343e46ac9c45e79deb81ba1dd45027799b3</citedby><cites>FETCH-LOGICAL-c445t-ee2f8d3c58dfbf5fa9b2cde322af9b343e46ac9c45e79deb81ba1dd45027799b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022522302000417$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13800779$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12091810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kofidis, T.</creatorcontrib><creatorcontrib>Akhyari, P.</creatorcontrib><creatorcontrib>Boublik, J.</creatorcontrib><creatorcontrib>Theodorou, P.</creatorcontrib><creatorcontrib>Martin, U.</creatorcontrib><creatorcontrib>Ruhparwar, A.</creatorcontrib><creatorcontrib>Fischer, S.</creatorcontrib><creatorcontrib>Eschenhagen, T.</creatorcontrib><creatorcontrib>Kubis, H.P.</creatorcontrib><creatorcontrib>Kraft, T.</creatorcontrib><creatorcontrib>Leyh, R.</creatorcontrib><creatorcontrib>Haverich, A.</creatorcontrib><title>In vitro engineering of heart muscle: Artificial myocardial tissue</title><title>The Journal of thoracic and cardiovascular surgery</title><addtitle>J Thorac Cardiovasc Surg</addtitle><description>Introduction: Myocardial infarction followed by heart failure represents one of the major causes of morbidity and mortality, particularly in industrialized countries. Engineering and subsequent transplantation of contractile artificial myocardial tissue and, consequently, the replacement of ischemic and infarcted areas of the heart provides a potential therapeutic alternative to whole organ transplantation. Methods: Artificial myocardial tissue samples were engineered by seeding neonatal rat cardiomyocytes with a commercially available 3-dimensional collagen matrix. The cellular engraftment within the artificial myocardial tissues was examined microscopically. Force development was analyzed in spontaneously beating artificial myocardial tissues, after stretching, and after pharmacologic stimulation. Moreover, electrocardiograms were recorded. Results: Artificial myocardial tissues showed continuous, rhythmic, and synchronized contractions for up to 13 weeks. Embedded cardiomyocytes were distributed equally within the 3-dimensional matrix. Application of Ca2+ and epinephrine, as well as electrical stimulation or stretching, resulted in enhanced force development. Electrocardiographic recording was possible on spontaneously beating artificial myocardial tissue samples and revealed physiologic patterns. Conclusions: Using a clinically well-established collagen matrix, contractile myocardial tissue can be engineered in vitro successfully. Mechanical and biologic properties of artificial myocardial tissue resemble native cardiac tissue. Use of artificial myocardial tissues might be a promising approach to reconstitute degenerated or failing cardiac tissue in many disease states and therefore provide a reasonable alternative to whole organ transplantation.
J Thorac Cardiovasc Surg 2002;124:63-9</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Collagen</subject><subject>Diseases of the cardiovascular system</subject><subject>Electric Stimulation</subject><subject>Electrocardiography</subject><subject>Medical sciences</subject><subject>Myocardial Contraction</subject><subject>Myocardium - cytology</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Tissue Engineering - methods</subject><issn>0022-5223</issn><issn>1097-685X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1rHDEQhkWIiS9O6nRhm7jbs0b7oVU62-TDYHCTQDqhHY3uZPbDkbQ2_vfRcgdXpRrBPPPy6mHsE_At8FZejQm3gnOxBQFKwhu2Aa5k2XbNn7dskxeibISoztn7GB8555KDesfOQXAFHfANu7mbimefwlzQtPMTUfDTrphdsScTUjEuEQf6WlyH5J1Hb4ZifJ3RBLs-k49xoQ_szJkh0sfjvGC_v3_7dfuzvH_4cXd7fV9iXTepJBKusxU2nXW9a5xRvUBLlRDGqb6qK6pbgwrrhqSy1HfQG7C2briQUmXigl0ecp_C_HehmPToI9IwmInmJWoJXbt-NoNXBxDDHGMgp5-CH0141cD1qk1nbXrVpg_a8sXnY_TSj2RP_NFTBr4cARPRDC6YCX08cVWX3Up16rj3u_2LD6TjaIYhx4J-TBhB1Bp0u3ZUB5CysWdPQUf0NCHZfIRJ29n_t-0_oZGW2g</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Kofidis, T.</creator><creator>Akhyari, P.</creator><creator>Boublik, J.</creator><creator>Theodorou, P.</creator><creator>Martin, U.</creator><creator>Ruhparwar, A.</creator><creator>Fischer, S.</creator><creator>Eschenhagen, T.</creator><creator>Kubis, H.P.</creator><creator>Kraft, T.</creator><creator>Leyh, R.</creator><creator>Haverich, A.</creator><general>Mosby, Inc</general><general>AATS/WTSA</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>In vitro engineering of heart muscle: Artificial myocardial tissue</title><author>Kofidis, T. ; Akhyari, P. ; Boublik, J. ; Theodorou, P. ; Martin, U. ; Ruhparwar, A. ; Fischer, S. ; Eschenhagen, T. ; Kubis, H.P. ; Kraft, T. ; Leyh, R. ; Haverich, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-ee2f8d3c58dfbf5fa9b2cde322af9b343e46ac9c45e79deb81ba1dd45027799b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Collagen</topic><topic>Diseases of the cardiovascular system</topic><topic>Electric Stimulation</topic><topic>Electrocardiography</topic><topic>Medical sciences</topic><topic>Myocardial Contraction</topic><topic>Myocardium - cytology</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kofidis, T.</creatorcontrib><creatorcontrib>Akhyari, P.</creatorcontrib><creatorcontrib>Boublik, J.</creatorcontrib><creatorcontrib>Theodorou, P.</creatorcontrib><creatorcontrib>Martin, U.</creatorcontrib><creatorcontrib>Ruhparwar, A.</creatorcontrib><creatorcontrib>Fischer, S.</creatorcontrib><creatorcontrib>Eschenhagen, T.</creatorcontrib><creatorcontrib>Kubis, H.P.</creatorcontrib><creatorcontrib>Kraft, T.</creatorcontrib><creatorcontrib>Leyh, R.</creatorcontrib><creatorcontrib>Haverich, A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of thoracic and cardiovascular surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kofidis, T.</au><au>Akhyari, P.</au><au>Boublik, J.</au><au>Theodorou, P.</au><au>Martin, U.</au><au>Ruhparwar, A.</au><au>Fischer, S.</au><au>Eschenhagen, T.</au><au>Kubis, H.P.</au><au>Kraft, T.</au><au>Leyh, R.</au><au>Haverich, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro engineering of heart muscle: Artificial myocardial tissue</atitle><jtitle>The Journal of thoracic and cardiovascular surgery</jtitle><addtitle>J Thorac Cardiovasc Surg</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>124</volume><issue>1</issue><spage>63</spage><epage>69</epage><pages>63-69</pages><issn>0022-5223</issn><eissn>1097-685X</eissn><coden>JTCSAQ</coden><abstract>Introduction: Myocardial infarction followed by heart failure represents one of the major causes of morbidity and mortality, particularly in industrialized countries. Engineering and subsequent transplantation of contractile artificial myocardial tissue and, consequently, the replacement of ischemic and infarcted areas of the heart provides a potential therapeutic alternative to whole organ transplantation. Methods: Artificial myocardial tissue samples were engineered by seeding neonatal rat cardiomyocytes with a commercially available 3-dimensional collagen matrix. The cellular engraftment within the artificial myocardial tissues was examined microscopically. Force development was analyzed in spontaneously beating artificial myocardial tissues, after stretching, and after pharmacologic stimulation. Moreover, electrocardiograms were recorded. Results: Artificial myocardial tissues showed continuous, rhythmic, and synchronized contractions for up to 13 weeks. Embedded cardiomyocytes were distributed equally within the 3-dimensional matrix. Application of Ca2+ and epinephrine, as well as electrical stimulation or stretching, resulted in enhanced force development. Electrocardiographic recording was possible on spontaneously beating artificial myocardial tissue samples and revealed physiologic patterns. Conclusions: Using a clinically well-established collagen matrix, contractile myocardial tissue can be engineered in vitro successfully. Mechanical and biologic properties of artificial myocardial tissue resemble native cardiac tissue. Use of artificial myocardial tissues might be a promising approach to reconstitute degenerated or failing cardiac tissue in many disease states and therefore provide a reasonable alternative to whole organ transplantation.
J Thorac Cardiovasc Surg 2002;124:63-9</abstract><cop>Philadelphia, PA</cop><pub>Mosby, Inc</pub><pmid>12091810</pmid><doi>10.1067/mtc.2002.121971</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Newborn Biological and medical sciences Collagen Diseases of the cardiovascular system Electric Stimulation Electrocardiography Medical sciences Myocardial Contraction Myocardium - cytology Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Rats Rats, Wistar Tissue Engineering - methods |
| title | In vitro engineering of heart muscle: Artificial myocardial tissue |
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