Amino, chloromethyl and acetal-functionalized latex particles for immunoassays: a comparative study
Latex particles with different functionalized surface groups (amino, acetal and chloromethyl) for the covalent linking of protein molecules were synthesized and characterized. Immunopurified anti-ferritin antibodies were then covalently coupled with a mean efficiency rate (protein covalently bound t...
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Veröffentlicht in: | Journal of immunological methods 2004-04, Vol.287 (1), p.159-167 |
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container_title | Journal of immunological methods |
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creator | Izquierdo, M.P.Sanz Martı́n-Molina, A. Ramos, J. Rus, A. Borque, L. Forcada, J. Galisteo-González, F. |
description | Latex particles with different functionalized surface groups (amino, acetal and chloromethyl) for the covalent linking of protein molecules were synthesized and characterized. Immunopurified anti-ferritin antibodies were then covalently coupled with a mean efficiency rate (protein covalently bound to latex particles with respect to the total amount of protein added) of 60%. The reagents developed were applied to the measurement of serum ferritin concentration in a turbidimetric procedure, showing a good measuring range and a lowest detection limit of 3.5 ng/ml in the case of the amino-modified particles. These immunological reagents were compared with a commercial nephelometric method, showing a good linear correlation in all cases but no transferability in the acetal and chloromethyl latexes with additional carboxyl groups, probably due to interference with other serum components. The differences among latexes found in this study indicate that it would be necessary to optimize the assay conditions for each type of particle, in order to achieve a maximum immunoreactivity. |
doi_str_mv | 10.1016/j.jim.2004.01.020 |
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Immunopurified anti-ferritin antibodies were then covalently coupled with a mean efficiency rate (protein covalently bound to latex particles with respect to the total amount of protein added) of 60%. The reagents developed were applied to the measurement of serum ferritin concentration in a turbidimetric procedure, showing a good measuring range and a lowest detection limit of 3.5 ng/ml in the case of the amino-modified particles. These immunological reagents were compared with a commercial nephelometric method, showing a good linear correlation in all cases but no transferability in the acetal and chloromethyl latexes with additional carboxyl groups, probably due to interference with other serum components. The differences among latexes found in this study indicate that it would be necessary to optimize the assay conditions for each type of particle, in order to achieve a maximum immunoreactivity.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2004.01.020</identifier><identifier>PMID: 15099764</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Ferritin ; Ferritins - blood ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunoassay ; Latex Fixation Tests - methods ; Method comparison ; Microscopy, Electron ; Microspheres ; Molecular immunology ; Nephelometry and Turbidimetry ; Sensitivity and Specificity ; Techniques ; Turbidimetry</subject><ispartof>Journal of immunological methods, 2004-04, Vol.287 (1), p.159-167</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-c561696cef36e78169def49be6e98d9b54d2e9e4f8a586cdf1db536dce78586c3</citedby><cites>FETCH-LOGICAL-c410t-c561696cef36e78169def49be6e98d9b54d2e9e4f8a586cdf1db536dce78586c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jim.2004.01.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15643937$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15099764$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Izquierdo, M.P.Sanz</creatorcontrib><creatorcontrib>Martı́n-Molina, A.</creatorcontrib><creatorcontrib>Ramos, J.</creatorcontrib><creatorcontrib>Rus, A.</creatorcontrib><creatorcontrib>Borque, L.</creatorcontrib><creatorcontrib>Forcada, J.</creatorcontrib><creatorcontrib>Galisteo-González, F.</creatorcontrib><title>Amino, chloromethyl and acetal-functionalized latex particles for immunoassays: a comparative study</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>Latex particles with different functionalized surface groups (amino, acetal and chloromethyl) for the covalent linking of protein molecules were synthesized and characterized. Immunopurified anti-ferritin antibodies were then covalently coupled with a mean efficiency rate (protein covalently bound to latex particles with respect to the total amount of protein added) of 60%. The reagents developed were applied to the measurement of serum ferritin concentration in a turbidimetric procedure, showing a good measuring range and a lowest detection limit of 3.5 ng/ml in the case of the amino-modified particles. These immunological reagents were compared with a commercial nephelometric method, showing a good linear correlation in all cases but no transferability in the acetal and chloromethyl latexes with additional carboxyl groups, probably due to interference with other serum components. The differences among latexes found in this study indicate that it would be necessary to optimize the assay conditions for each type of particle, in order to achieve a maximum immunoreactivity.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Ferritin</subject><subject>Ferritins - blood</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Latex Fixation Tests - methods</subject><subject>Method comparison</subject><subject>Microscopy, Electron</subject><subject>Microspheres</subject><subject>Molecular immunology</subject><subject>Nephelometry and Turbidimetry</subject><subject>Sensitivity and Specificity</subject><subject>Techniques</subject><subject>Turbidimetry</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U2LFDEQBuAgiju7-gO8SC562m4r_ZHuuKdl8QsWvOg5ZJIKmyHpjEl6cfz1ZpgBPekpRXiqKOol5BWDlgHj73btzoW2AxhaYC108IRs2Dx1zSRgfEo2AF3XsGkUF-Qy5x0AMODwnFywEYSY-LAh-ja4JV5T_eBjigHLw8FTtRiqNBblG7suuri4KO9-oaFeFfxJ9yoVpz1mamOiLoR1iSpndcjvqaI6hgpUcY9Ic1nN4QV5ZpXP-PL8XpHvHz98u_vc3H_99OXu9r7RA4PS6JEzLrhG23Oc5lobtIPYIkcxG7EdB9OhwMHOapy5NpaZ7dhzoys-fvRX5O1p7j7FHyvmIoPLGr1XC8Y1y4lVN7P-v5DN0PF-EBWyE9Qp5pzQyn1yQaWDZCCPEcidrBHIYwQSmKwR1J7X5-HrNqD503G-eQVvzkBlrbxNatEu_-WqEf1U3c3JYb3Zo8Mks3a4aDQuoS7SRPePNX4D42qlxw</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Izquierdo, M.P.Sanz</creator><creator>Martı́n-Molina, A.</creator><creator>Ramos, J.</creator><creator>Rus, A.</creator><creator>Borque, L.</creator><creator>Forcada, J.</creator><creator>Galisteo-González, F.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Amino, chloromethyl and acetal-functionalized latex particles for immunoassays: a comparative study</title><author>Izquierdo, M.P.Sanz ; Martı́n-Molina, A. ; Ramos, J. ; Rus, A. ; Borque, L. ; Forcada, J. ; Galisteo-González, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-c561696cef36e78169def49be6e98d9b54d2e9e4f8a586cdf1db536dce78586c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Ferritin</topic><topic>Ferritins - blood</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Latex Fixation Tests - methods</topic><topic>Method comparison</topic><topic>Microscopy, Electron</topic><topic>Microspheres</topic><topic>Molecular immunology</topic><topic>Nephelometry and Turbidimetry</topic><topic>Sensitivity and Specificity</topic><topic>Techniques</topic><topic>Turbidimetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Izquierdo, M.P.Sanz</creatorcontrib><creatorcontrib>Martı́n-Molina, A.</creatorcontrib><creatorcontrib>Ramos, J.</creatorcontrib><creatorcontrib>Rus, A.</creatorcontrib><creatorcontrib>Borque, L.</creatorcontrib><creatorcontrib>Forcada, J.</creatorcontrib><creatorcontrib>Galisteo-González, F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Izquierdo, M.P.Sanz</au><au>Martı́n-Molina, A.</au><au>Ramos, J.</au><au>Rus, A.</au><au>Borque, L.</au><au>Forcada, J.</au><au>Galisteo-González, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amino, chloromethyl and acetal-functionalized latex particles for immunoassays: a comparative study</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>287</volume><issue>1</issue><spage>159</spage><epage>167</epage><pages>159-167</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>Latex particles with different functionalized surface groups (amino, acetal and chloromethyl) for the covalent linking of protein molecules were synthesized and characterized. Immunopurified anti-ferritin antibodies were then covalently coupled with a mean efficiency rate (protein covalently bound to latex particles with respect to the total amount of protein added) of 60%. The reagents developed were applied to the measurement of serum ferritin concentration in a turbidimetric procedure, showing a good measuring range and a lowest detection limit of 3.5 ng/ml in the case of the amino-modified particles. These immunological reagents were compared with a commercial nephelometric method, showing a good linear correlation in all cases but no transferability in the acetal and chloromethyl latexes with additional carboxyl groups, probably due to interference with other serum components. The differences among latexes found in this study indicate that it would be necessary to optimize the assay conditions for each type of particle, in order to achieve a maximum immunoreactivity.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15099764</pmid><doi>10.1016/j.jim.2004.01.020</doi><tpages>9</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Ferritin Ferritins - blood Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunoassay Latex Fixation Tests - methods Method comparison Microscopy, Electron Microspheres Molecular immunology Nephelometry and Turbidimetry Sensitivity and Specificity Techniques Turbidimetry |
title | Amino, chloromethyl and acetal-functionalized latex particles for immunoassays: a comparative study |
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