Amino, chloromethyl and acetal-functionalized latex particles for immunoassays: a comparative study

Latex particles with different functionalized surface groups (amino, acetal and chloromethyl) for the covalent linking of protein molecules were synthesized and characterized. Immunopurified anti-ferritin antibodies were then covalently coupled with a mean efficiency rate (protein covalently bound t...

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Veröffentlicht in:Journal of immunological methods 2004-04, Vol.287 (1), p.159-167
Hauptverfasser: Izquierdo, M.P.Sanz, Martı́n-Molina, A., Ramos, J., Rus, A., Borque, L., Forcada, J., Galisteo-González, F.
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container_end_page 167
container_issue 1
container_start_page 159
container_title Journal of immunological methods
container_volume 287
creator Izquierdo, M.P.Sanz
Martı́n-Molina, A.
Ramos, J.
Rus, A.
Borque, L.
Forcada, J.
Galisteo-González, F.
description Latex particles with different functionalized surface groups (amino, acetal and chloromethyl) for the covalent linking of protein molecules were synthesized and characterized. Immunopurified anti-ferritin antibodies were then covalently coupled with a mean efficiency rate (protein covalently bound to latex particles with respect to the total amount of protein added) of 60%. The reagents developed were applied to the measurement of serum ferritin concentration in a turbidimetric procedure, showing a good measuring range and a lowest detection limit of 3.5 ng/ml in the case of the amino-modified particles. These immunological reagents were compared with a commercial nephelometric method, showing a good linear correlation in all cases but no transferability in the acetal and chloromethyl latexes with additional carboxyl groups, probably due to interference with other serum components. The differences among latexes found in this study indicate that it would be necessary to optimize the assay conditions for each type of particle, in order to achieve a maximum immunoreactivity.
doi_str_mv 10.1016/j.jim.2004.01.020
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subjects Animals
Biological and medical sciences
Ferritin
Ferritins - blood
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunoassay
Latex Fixation Tests - methods
Method comparison
Microscopy, Electron
Microspheres
Molecular immunology
Nephelometry and Turbidimetry
Sensitivity and Specificity
Techniques
Turbidimetry
title Amino, chloromethyl and acetal-functionalized latex particles for immunoassays: a comparative study
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