The upstream Sal repeat-containing segment of Arabidopsis thaliana ribosomal DNA intergenic region (IGR) enhances the activity of adjacent protein-coding genes
The sequence containing 'upstream Sal repeats' (USR) from the Arabidopsis thaliana ribosomal DNA intergenic region (IGR) was tested for its influence on the in vivo activity of nearby protein coding genes. On average, the presence of the IGR fragment leads to a four-fold increase in the ex...
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| Veröffentlicht in: | Plant molecular biology 2002-08, Vol.49 (6), p.655-663 |
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| container_title | Plant molecular biology |
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| creator | Schlögelhofer, Peter Nizhynska, Victoria Feik, Nicholas Chambon, Céline Potuschak, Thomas Wanzenböck, Eva-Maria Schweizer, Dieter Bachmair, Andreas |
| description | The sequence containing 'upstream Sal repeats' (USR) from the Arabidopsis thaliana ribosomal DNA intergenic region (IGR) was tested for its influence on the in vivo activity of nearby protein coding genes. On average, the presence of the IGR fragment leads to a four-fold increase in the expression of a reporter gene, beta-glucuronidase, under control of the strong CaMV 35S promoter. With the help of the site-specific cre-lox recombination system, we have also obtained pairs of transgenic lines with or without the USR-containing fragment, both integrated at the same chromosomal position. Results with these transgenic lines, which contain an NPT II (kanamycin resistance) gene under control of the nos promoter as a test gene, confirmed the results obtained with the CaMV 35S-driven GUS gene. Moreover, they show that the IGR sequence can oppose tendencies of gene silencing. We hypothesize that the described effect relates to features of the chromatin structure in the proximity of the upstream Sal repeats. |
| doi_str_mv | 10.1023/A:1015556531074 |
| format | Article |
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On average, the presence of the IGR fragment leads to a four-fold increase in the expression of a reporter gene, beta-glucuronidase, under control of the strong CaMV 35S promoter. With the help of the site-specific cre-lox recombination system, we have also obtained pairs of transgenic lines with or without the USR-containing fragment, both integrated at the same chromosomal position. Results with these transgenic lines, which contain an NPT II (kanamycin resistance) gene under control of the nos promoter as a test gene, confirmed the results obtained with the CaMV 35S-driven GUS gene. Moreover, they show that the IGR sequence can oppose tendencies of gene silencing. We hypothesize that the described effect relates to features of the chromatin structure in the proximity of the upstream Sal repeats.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1015556531074</identifier><identifier>PMID: 12081373</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; DNA, Ribosomal Spacer - genetics ; Gene Expression Regulation, Plant ; Genes ; Genetics ; Glucuronidase - genetics ; Glucuronidase - metabolism ; Plant Leaves - genetics ; Plants, Genetically Modified ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Repetitive Sequences, Nucleic Acid - genetics ; Ribosomal DNA</subject><ispartof>Plant molecular biology, 2002-08, Vol.49 (6), p.655-663</ispartof><rights>Kluwer Academic Publishers 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-f1a252cb20309cc2053bcf9c3a9c4047089c121e3c78b32cf35269c032e106593</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12081373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schlögelhofer, Peter</creatorcontrib><creatorcontrib>Nizhynska, Victoria</creatorcontrib><creatorcontrib>Feik, Nicholas</creatorcontrib><creatorcontrib>Chambon, Céline</creatorcontrib><creatorcontrib>Potuschak, Thomas</creatorcontrib><creatorcontrib>Wanzenböck, Eva-Maria</creatorcontrib><creatorcontrib>Schweizer, Dieter</creatorcontrib><creatorcontrib>Bachmair, Andreas</creatorcontrib><title>The upstream Sal repeat-containing segment of Arabidopsis thaliana ribosomal DNA intergenic region (IGR) enhances the activity of adjacent protein-coding genes</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>The sequence containing 'upstream Sal repeats' (USR) from the Arabidopsis thaliana ribosomal DNA intergenic region (IGR) was tested for its influence on the in vivo activity of nearby protein coding genes. 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We hypothesize that the described effect relates to features of the chromatin structure in the proximity of the upstream Sal repeats.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genetics</subject><subject>Glucuronidase - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>Plant Leaves - genetics</subject><subject>Plants, Genetically Modified</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Ribosomal DNA</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkcFu1DAQhi0EokvhzA1ZHBAcAjN2HCfcVgVKpQokKOfI8U52vUrs1HaQ-jS8Kl5RLlw4zeX7v39Gw9hzhLcIQr7bvkdApVSjJIKuH7ANKi0rBaJ9yDaAja7qGsUZe5LSEQABZPOYnaGAFqWWG_br5kB8XVKOZGb-3Uw80kImVzb4bJx3fs8T7WfymYeRb6MZ3C4sySWeD2Zyxhse3RBSmEv2w5ctdz5T3JN3tqj2Lnj--ury2xtO_mC8pVOOuLHZ_XT57uQ0u6OxJ_8SQybnS_XuVFsclJ6yR6OZEj27n-fsx6ePNxefq-uvl1cX2-vKSgm5GtEIJewgQEJnrQAlBzt2VprO1lBraDuLAkla3Q5S2FEq0XQWpCCERnXynL364y1L3K6Ucj-7ZGmajKewpl5jq5RuxH9BbGvArtEFfPkPeAxr9OWIXjdt0bW1LNCLe2gdZtr1S3SziXf93wfJ36z8ksk</recordid><startdate>200208</startdate><enddate>200208</enddate><creator>Schlögelhofer, Peter</creator><creator>Nizhynska, Victoria</creator><creator>Feik, Nicholas</creator><creator>Chambon, Céline</creator><creator>Potuschak, Thomas</creator><creator>Wanzenböck, Eva-Maria</creator><creator>Schweizer, Dieter</creator><creator>Bachmair, Andreas</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200208</creationdate><title>The upstream Sal repeat-containing segment of Arabidopsis thaliana ribosomal DNA intergenic region (IGR) enhances the activity of adjacent protein-coding genes</title><author>Schlögelhofer, Peter ; Nizhynska, Victoria ; Feik, Nicholas ; Chambon, Céline ; Potuschak, Thomas ; Wanzenböck, Eva-Maria ; Schweizer, Dieter ; Bachmair, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-f1a252cb20309cc2053bcf9c3a9c4047089c121e3c78b32cf35269c032e106593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genetics</topic><topic>Glucuronidase - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>Plant Leaves - genetics</topic><topic>Plants, Genetically Modified</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Ribosomal DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schlögelhofer, Peter</creatorcontrib><creatorcontrib>Nizhynska, Victoria</creatorcontrib><creatorcontrib>Feik, Nicholas</creatorcontrib><creatorcontrib>Chambon, Céline</creatorcontrib><creatorcontrib>Potuschak, Thomas</creatorcontrib><creatorcontrib>Wanzenböck, Eva-Maria</creatorcontrib><creatorcontrib>Schweizer, Dieter</creatorcontrib><creatorcontrib>Bachmair, Andreas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schlögelhofer, Peter</au><au>Nizhynska, Victoria</au><au>Feik, Nicholas</au><au>Chambon, Céline</au><au>Potuschak, Thomas</au><au>Wanzenböck, Eva-Maria</au><au>Schweizer, Dieter</au><au>Bachmair, Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The upstream Sal repeat-containing segment of Arabidopsis thaliana ribosomal DNA intergenic region (IGR) enhances the activity of adjacent protein-coding genes</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>2002-08</date><risdate>2002</risdate><volume>49</volume><issue>6</issue><spage>655</spage><epage>663</epage><pages>655-663</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>The sequence containing 'upstream Sal repeats' (USR) from the Arabidopsis thaliana ribosomal DNA intergenic region (IGR) was tested for its influence on the in vivo activity of nearby protein coding genes. 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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Arabidopsis - genetics Arabidopsis Proteins - genetics DNA, Ribosomal Spacer - genetics Gene Expression Regulation, Plant Genes Genetics Glucuronidase - genetics Glucuronidase - metabolism Plant Leaves - genetics Plants, Genetically Modified Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Repetitive Sequences, Nucleic Acid - genetics Ribosomal DNA |
| title | The upstream Sal repeat-containing segment of Arabidopsis thaliana ribosomal DNA intergenic region (IGR) enhances the activity of adjacent protein-coding genes |
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