cDNA Cloning of the Sry-Related Gene Sox6 from Rat with Tissue-Specific Expression

A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this cDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectiv...

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Veröffentlicht in:	Biological & pharmaceutical bulletin 2002, Vol.25(6), pp.705-709
Hauptverfasser:	Narahara, Masanori, Yamada, Akihisa, Hamada-Kanazawa, Michiko, Kawai, Yuichi, Miyake, Masaharu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Biological and medical sciences Biotechnology Blotting, Northern Cell Line DNA, Complementary - biosynthesis DNA, Complementary - genetics DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics High Mobility Group Proteins - biosynthesis High Mobility Group Proteins - genetics In vitro gene amplification. Pcr technique Male Methods. Procedures. Technologies Organ Specificity polymerase chain reaction (PCR) cloning Rats Real-time quantitative reverse-transcription (RT)-PCR Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Sox6 SOXD Transcription Factors sry Testis - metabolism Tissue Distribution Transcription Factors Tretinoin - metabolism
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